dictyBase, the model organism database for Dictyostelium discoideum

dictyBase () is the model organism database (MOD) for the social amoeba Dictyostelium discoideum. The unique biology and phylogenetic position of Dictyostelium offer a great opportunity to gain knowledge of processes not characterized in other organisms. The recent completion of the 34 MB genome sequence, together with the sizable scientific literature using Dictyostelium as a research organism, provided the necessary tools to create a well-annotated genome. dictyBase has leveraged software developed by the Saccharomyces Genome Database and the Generic Model Organism Database project. This has reduced the time required to develop a full-featured MOD and greatly facilitated our ability to focus on annotation and providing new functionality. We hope that manual curation of the Dictyostelium genome will facilitate the annotation of other genomes

The dictyostelium kinome--analysis of the protein kinases from a simple model organism

Dictyostelium discoideum is a widely studied model organism with both unicellular and multicellular forms in its developmental cycle. The Dictyostelium genome encodes 285 predicted protein kinases, similar to the count of the much more advanced Drosophila. It contains members of most kinase classes shared by fungi and metazoans, as well as many previously thought to be metazoan specific, indicating that they have been secondarily lost from the fungal lineage. This includes the entire tyrosine kinase–like (TKL) group, which is expanded in Dictyostelium and includes several novel receptor kinases. Dictyostelium lacks tyrosine kinase group kinases, and most tyrosine phosphorylation appears to be mediated by TKL kinases. About half of Dictyostelium kinases occur in subfamilies not present in yeast or metazoa, suggesting that protein kinases have played key roles in the adaptation of Dictyostelium to its habitat. This study offers insights into kinase evolution and provides a focus for signaling analysis in this system

Body projects and the regulation of normative masculinity

Drawing on interviews with 140 young British males, this paper explores the ways in which men talk about their own bodies and bodily practices, and those of other men. The specific focus of interest is a variety of body modification practices, including working out (at a gym) tattooing, piercing and cosmetic surgery. We want to argue, however, that the significance of this analysis extends beyond the topic of body modification to a broader set of issues concerned with the nature of men’s embodied identities. In discussing the appearance of their bodies, the men we interviewed talked less about muscle and skin than about their own selves located within particular social, cultural and moral universes. The surfaces of their bodies were, as Mike Featherstone (1991) has argued, charged primarily with ‘identity functions’, allowing men to establish a place for themselves in contemporary society. Using a social psychological approach which can be characterised as a discursive analysis (Henwood, Gill & McLean, 1999; Lupton, 1998), this paper makes connections between men’s private feelings and bodily practices, and broader social and cultural trends and relations. It shows that in talking about seemingly trivial questions such as whether to have one’s nose pierced or whether to join a gym, men are actively engaged in constructing and policing appropriate masculine behaviours and identities; above all, in regulating normative masculinity. We identify five key discourses or ‘interpretive repertoires’ (Wetherell & Potter, 1992) which together construct the meanings for these men of attempts to modify the appearance of the body. The five discourses or repertoires were focused on the themes of individualism and ‘being different’; libertarianism and the autonomous body; unselfconsciousness and the rejection of vanity; a notion of the ‘well-balanced’ and unobsessional self; and self-respect and the morally accountable body. Our analysis lends support to the claim that the body has become a new (identity) project in high/late/postmodernity (e.g. Shilling, 1993; Featherstone, 1991), but shows how fraught with difficulties this project is for young men who must simultaneously work on and discipline their bodies while disavowing any (inappropriate) interest in their own appearance. The analysis highlights the pervasive individualism of young men’s discourses, and the absence of alternative ways of making sense of embodied experiences