Metod Kaledina i Romanenki v issledovanijakh zagrjaznenijj potrebitel'nykh predmetov jajjcami parazitov

Examinations were made of household objects contaminated with eggs of Enterobius vermicularis, Ascaris lumbricoides, Trichuris trichiura by using a device, joined to a vacuum cleaner, worked out by Kaledin and Romanienko and modified by the authors. The method by Kaledin and Romanienko has been found to be simple, rapid and effective because in the course of collecting samples, ready microscopic preparations are obtained. The obtained results concerning the degree of contamination of various objects with eggs of parasites are in agreement with the mechanism of transmission of eggs found in the environment and are in accordance with the observations of other authors. It seems that the method of Kaledin and Romanienko may be used in complex parasitic examinations

Infekcija detskojj ostricy (Enterobius vermicularis) i rezul'taty biokhimicheskogo diagnoza askaridoza

A hundred children from a primary school at Lublin, in whom Enterobius vermicularis was detected by Graham's method, were examined for the presence of volatile fatty acids in the urine by Soprunova's method. In the same children coproscopic examinations were carried out by the method of direct smear and by Riva's and Faust's methods. The obtained results show that E. vermicularis does not excrete volatile fatty acids characteristic of Ascaris, which means that infecticon by E. vermicularis does not affect the results of biochemical diagnostics of ascaridosis. The results confirm the specificity of Soprunova's method and point at its possible use in field examinations

Electrophoretic characteristics of soluble proteins of Fasciola hepatica (Linnaeus, 1758)

Proteins were extracted from F. hepatica collected on cattle and sheep. The extract was purified in the column with sephadex G-25. The proteins were separated using the Davis method in electrophoretic systems constructed by the authors, in the columns and in blocks in polyacrylamid gel. The electrophoregrams served for making densometric diagrams and for calculating electrophoretic mobility coefficient (Rf) of the identified protein bands. Both in the blocks and in the columns 26 protein bands have been identified in F. hepatica from both cattle and sheep. Statistical analysis with t Student test showed significant differences in their Rf values. A high reproducibility of the results has been obtained both in the columns and in the blocks of polyacrilamid gel

Iz kazuistiki strongiloidoza

Because of strongyloidosis two patients still living in Poland and one foreigner were treated. Diagnose has been based on the Fillleborn agar culture but behaviour of the parasite and its larvae during treatment was controlled with Baermann method in addition. During treatment telmid has been administered for two patients but thiabendazol (mintezol) for the third. The effectiveness of therapy has been controlled using the methods mentioned above after the first and the sixth month from the date of cure ending. There has been obtained the permanent therapeutical effect