44 research outputs found
Jules Verne 2.0, renewal of a large wind tunnel facility
International audienceThe paper aims at showing the evolution of methods to design a large wind tunnel. The current Jules Verne facility was designed with a scale model of the wind tunnel which enabled hot wire local wind speed measurements. The new facility is designed according a numerical modelling approach which parameters were validated by PIV measurements in the reduced scale physical model
Development of a preclinical model of donation after circulatory determination of death for translational application
BACKGROUND: Extracorporeal membranous oxygenation is proposed for abdominal organ procurement from donation after circulatory determination of death (DCD). In France, the national Agency of Biomedicine supervises the procurement of kidneys from DCD, specifying the durations of tolerated warm and cold ischemia. However, no study has determined the optimal conditions of this technique. The aim of this work was to develop a preclinical model of DCD using abdominal normothermic oxygenated recirculation (ANOR). In short, our objectives are to characterize the mechanisms involved during ANOR and its impact on abdominal organs. METHODS: We used Large White pigs weighing between 45 and 55Â kg. After 30Â minutes of potassium-induced cardiac arrest, the descending thoracic aorta was clamped and ANOR set up between the inferior vena cava and the abdominal aorta for 4Â hours. Hemodynamic, respiratory and biochemical parameters were collected. Blood gasometry and biochemistry analysis were performed during the ANOR procedure. RESULTS: Six ANOR procedures were performed. The surgical procedure is described and intraoperative parameters and biological data are presented. Pump flow rates were between 2.5 and 3Â l/min. Hemodynamic, respiratory, and biochemical objectives were achieved under reproducible conditions. Interestingly, animals remained hemodynamically stable following the targeted protocol. Arterial pH was controlled, and natremia and renal function remained stable 4Â hours after the procedure was started. Decreased hemoglobin and serum proteins levels, concomitant with increased lactate dehydrogenase activity, were observed as a consequence of the surgery. The serum potassium level was increased, owing to the extracorporeal circulation circuit. CONCLUSIONS: Our ANOR model is the closest to clinical conditions reported in the literature and will allow the study of the systemic and abdominal organ impact of this technique. The translational relevance of the pig will permit the determination of new biomarkers and protocols to improve DCD donor management
Inhibition of complement improves graft outcome in a pig model of kidney autotransplantation
Champignon parfume (ou shii-take) : une methode francaise pour sa culture
National audienc
Champignon parfume (ou shii-take) : une methode francaise pour sa culture
National audienc
Sviluppi nella coltivazione dei funghi alternativi, in particolare di Pleurotus e Shiitake
International audienc