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International audienceSensors based on surface plasmon resonance have the potential to provide information on the binding of biological molecules on adequate substrates over typically thousand channels in parallel, without the need for any marker and in real time compared to the scale of biochemical reactions. The need to optimize selectivity and sensitivity has triggered continued research efforts. We review those related with optics and image processing, at the same time identifying some aspects that deserve further investigation before the potential of the technique is fully utilized

Generalization Of The Rouard Method To An Absorbing Thin-Film Stack And Application To Surface Plasmon Resonance

In the context of surface plasmon resonance (SPR) kinetic biochips, it is important to model the SPR phenomenon (i.e., extinction of reflectivity) toward biochip design and optimization. The Rouard approach that models reflectivity off a thin-film stack is shown to be extendable to any number of absorbing layers with no added complexity. Using the generalized Rouard method, the effect of SPR is simulated as a function of the wavelength for various metal thicknesses. Given an optimal metal thickness, the dependence of SPR on the angle of incidence and wavelength is also demonstrated. Such a model constitutes a potential basis for the efficient design and optimization of multidimensional sensors. © 2006 Optical Society of America

Surface plasmon resonance imaging as a multidimensional surface characterization instrument - application to biochip genotyping

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Multispectral imaging of a biochip based surface plasmon resonance

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Surface plasmon resonance imaging (SPRI) system and real-time monitoring of DNA biochip for human genetic mutation diagnosis of DNA amplified samples

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