Citomegalovirus nei pazienti trapiantati d\u2019organo

aim of the work Calprotectin (CalP) and myeloperoxidase (MPO) are biochemical markers in the gingival crevicular fluid (GCF). Their increased concentration indicates the onset of an inflammatory process. The aim of this study was to valuate the correlation between the levels of calprotectin and myeloperoxidase detected in the crevicular fluid (GCF) at different probing depth (PPD). Materials and methods In a total of 11 subjects with chronic periodontitis, non smokers enjoying good health and who had not undergone periodontal nor antibiotic therapy in the 6 months prior the study, 60 sites were randomly selected and bleeding on probing (BOP), PPD, quantity of GCF and concentration of MPO and CalP were recorded. The volume of GCF was assessed through inserting PerioCol paper strips in the periodontal pockets for 10 seconds, then analyzed with a micro-moisture meter electronic instrument (Periotrom 6000, Siemens). The determination of the CalP was performed by enzyme immunoassay. The determination of MPO in the crevicular fluid was performed using the spectrophotometric reading of optical density. The results were statistically analyzed (SPSS16). Results PPD 5 mm (GCF=0,72 \u3bcg/\u3bcl; CalP=10,06 \u3bcg/\u3bcl; MPO=3,69 \u3bcg/\u3bcl); PPD 6 mm (GCF=0,78 \u3bcg/\u3bcl; CalP=10,64 \u3bcg/\u3bcl; MPO=5,45 \u3bcg/\u3bcl); PPD 657 mm (GCF=1,32 \u3bcg/\u3bcl; CalP=7,20 \u3bcg/\u3bcl; MPO=5,55 \u3bcg/\u3bcl). There were no statistically significant differences in the concentrations of MPO and CalP in the deeper periodontal pocket. Conclusion The concentration ofmyeloperoxidase seems significantly related to the increased presence of neutrophils into the deepest pockets

Sylvatic rabies in the North Est of Italy: monitoring and evaluation of the effectiveness of prophylaxis in workers at risk and travelers

Rabies is a zoonotic viral encephalitis almost invariably fatal in both humans and animals. Rabies virus infects mammals through infected saliva via bites or scratches, although atypical exposures have been documented. In late 2008, wildlife rabies re emerged in Northeastern Italy. Since then, 287 animal cases have been documented with the last one diagnosed in a red fox in February 2011. No human cases have been reported linked to the recent epidemic and Italy has been declared as free from rabies in February 2013. Several oral fox vaccination campaigns and extensive monitoring of territories subject to the epidemic have been implemented together with education and preventive vaccination of workers at risk of viral exposure as forestry and wildlife workers, veterinarians, shelters \u2018operators and laboratory personnel. We have evaluated the level and persistence of serological response in workers at risk and travelers vaccinated following different immunization schedules. In 113 cases post exposure prophylaxis has been administered, five or four doses, with Rabipur (Novartis), Verorab (Sanofi Pasteur) vaccine. Serum samples from about 300 vaccinated volunteers at risk and travelers were collected from the Northeastern territories recently subject to the epidemic. All sera have been tested using an ELISA (Enzyme Linked Immunosorbent Assay) and a neutralising test, namely FAVN (Fluorescent Antibody Virus Neutralisation). The results of the ongoing analysis will be reported