Utilization of lime for stabilizing soft clay soil of high organic content

This paper presents the results of geotechnical and mineralogical investigations on lime-treated soft clay soil from Idku City, Egypt, where high organic matters of about 14% exist. Lime was added in the order of 1, 3, 5 and 7% by weight and laboratory experiments after 7, 15, 30 and 60 days were conducted including the mineralogical and microstructural examinations, grain size analysis, plasticity limits, unconfined compressive tests, vane shear tests and oedometer tests. The results indicate that soft clay soil of high organic content of 14% can be stabilized satisfactorily with the addition of 7% lime. The results also demonstrate that the changes in the mineralogical contents and soil fabric of high organic lime-treated soft clay improve soil plasticity, strength and compressibility

Low recurrence rate of a two-layered closure repair for primary and recurrent midline incisional hernia without mesh

Background: Incisional hernia is a serious complication after abdominal surgery and occurs in 11-23% of laparotomies. Repair can be done, for instance, with a direct suture technique, but recurrence rates are high. Recent literature advises the use of mesh repair. In contrast to this development, we studied the use of a direct suture repair in a separate layer technique. The objective of this retrospective observational study is to assess the outcomes (recurrences and complications) of a two-layered open closure repair for primary and recurrent midline incisional hernia without the use of mesh. Methods: In an observational retrospective cohort study, we analysed the hospital and outpatient records of 77 consecutive patients who underwent surgery for a primary or recurrent incisional hernia between 1st May 2002 and 8th November 2006. The repair consisted of separate continuous suturing of the anterior and posterior fascia, including the rectus muscle, after extensive intra-abdominal adhesiolysis. Results: Forty-one men (53.2%) and 36 women (46.8%) underwent surgery. Sixty-three operations (81.8%) were primary repairs and 14 (18.2%) were repairs for a recurrent incisional hernia. Of the 66 patients, on physical examination, three had a recurrence (4.5%) after an average follow-up of 2.6 years. The 30-day postoperative mortality was 1.1%. Wound infection was seen in five patients (6.5%). Conclusions: A two-layered suture repair for primary and recurrent incisional hernia repair without mesh with extensive adhesiolysis was associated with a recurrence rate comparable to mesh repair and had an acceptable complication rate

Low dose radiation and cancer in A-bomb survivors: latency and non-linear dose-response in the 1950–90 mortality cohort

BACKGROUND: Analyses of Japanese A-bomb survivors' cancer mortality risks are used to establish recommended annual dose limits, currently set at 1 mSv (public) and 20 mSv (occupational). Do radiation doses below 20 mSv have significant impact on cancer mortality in Japanese A-bomb survivors, and is the dose-response linear? METHODS: I analyse stomach, liver, lung, colon, uterus, and all-solid cancer mortality in the 0 – 20 mSv colon dose subcohort of the 1950–90 (grouped) mortality cohort, by Poisson regression using a time-lagged colon dose to detect latency, while controlling for gender, attained age, and age-at-exposure. I compare linear and non-linear models, including one adapted from the cellular bystander effect for α particles. RESULTS: With a lagged linear model, Excess Relative Risk (ERR) for the liver and all-solid cancers is significantly positive and several orders of magnitude above extrapolations from the Life Span Study Report 12 analysis of the full cohort. Non-linear models are strongly superior to the linear model for the stomach (latency 11.89 years), liver (36.90), lung (13.60) and all-solid (43.86) in fitting the 0 – 20 mSv data and show significant positive ERR at 0.25 mSv and 10 mSv lagged dose. The slope of the dose-response near zero is several orders of magnitude above the slope at high doses. CONCLUSION: The standard linear model applied to the full 1950–90 cohort greatly underestimates the risks at low doses, which are significant when the 0 – 20 mSv subcohort is modelled with latency. Non-linear models give a much better fit and are compatible with a bystander effect

Modeling Symmetric Macromolecular Structures in Rosetta3

Symmetric protein assemblies play important roles in many biochemical processes. However, the large size of such systems is challenging for traditional structure modeling methods. This paper describes the implementation of a general framework for modeling arbitrary symmetric systems in Rosetta3. We describe the various types of symmetries relevant to the study of protein structure that may be modeled using Rosetta's symmetric framework. We then describe how this symmetric framework is efficiently implemented within Rosetta, which restricts the conformational search space by sampling only symmetric degrees of freedom, and explicitly simulates only a subset of the interacting monomers. Finally, we describe structure prediction and design applications that utilize the Rosetta3 symmetric modeling capabilities, and provide a guide to running simulations on symmetric systems

Language learning and integration of adult Bhutanese refugees : an ethnographic study

This study sought a holistic and in-depth investigation of English language learning and integration experiences of a group of adult Bhutanese refugees in Australia. The Bhutanese refugees have settled since 2007 as new residents of their host country after two decades of expatriate life in the refugee camps of Nepal. The impetus for this exploratory study stemmed from my personal experience as a cultural orientation trainer to such refugees and awareness of their expectations, attitudes, and dispositions related to learning and life trajectory. This study is interdisciplinary in its approach that takes account of the complex interplay of language learning and integration. Using an ethnographic methodology as an approach to investigation, this study sought to examine three social spaces of refugees: the family and ethnic community, the host society and the migrant English classroom. The attention was focussed on the resources and constraints the refugees encountered in each of the social spaces. Moreover, various social, contextual, cultural factors, and pre-migration influences embedded in these spaces were explored for their impact on learning and integration. This study was important mainly for three reasons. Through critical examination of the role of refugees’ family and ethnic community networks, it aimed to provide insights into to what extent the resources embedded in these networks can be significant to refugees and also contribute to the existing literature of social capital (Putnam, 2000). Drawing on the investigation of how cultural issues, pre-migration experiences and perceptions of teaching impact on classroom language learning, this study sought to offer insights into how the English language should be taught to the adult students from refugee backgrounds. Moreover, this study sought to extend the scope of the existing refugee integration literature by investigating the integration as a process of ongoing negotiation between ethnocultural retention and host society participation, and how various social, cultural, contextual factors, and pre-migration experiences influenced the way integration is structured in everyday practice. This study employed an ethnographic approach as a methodological framework, involving observations, interviews, and a reflective journal study as the main tools for data collection. The field work was carried out in a regional area of the State of Tasmania. The observations were conducted in the migrant English classrooms, in a multi-ethnic Australian church and in the refugee community; and then the retrospective interviews were carried out with the Bhutanese refugees, their teachers and other service providers. The data were analyzed using ethnographic macro and micro level analysis techniques (Duff, 2002). The findings generated from observation data were supported and triangulated, where possible, by the use of data derived from interviews. This study was informed mainly by the interpretive paradigm. Given how extensive and messy the literature on migrant language learning and integration is, this study utilized a wide range of relevant theories to analyze and interpret the findings derived from the ethnographic fieldwork. One important contribution of this study is the finding that the social capital derived from refugees’ family and ethnic community networks not only enables, but it also inhibits integration. This bonding social capital can function as a coping resource for refugees against the effects of culture shock, language shock, and racism, and can facilitate access to a range of instrumental support and information necessary for successful transition to the host society. However, this study also suggests that an extreme level of embeddedness within the cultural and social frames of ethnic space has the potential to jeopardize individual mobility, host society language learning and sustainable integration. This study shows that the cultural dispositions the adult refugee students bring to the classroom provide the primary basis for the way they approach their English learning, and thus influence their agency and identity as learners. The findings also suggest that the students are likely to engage in the desired learning tasks if their perceptions of teaching quality cohere with the actual teaching they are exposed to. Teachers are therefore suggested to adopt a hybridity of teaching approaches and methods in ways that bridge the gap between their own and their students’ perceptions and expectations. The implications drawn from the empirical results additionally suggest that if the aim of the migrant English program is to facilitate the integration of refugees into their multicultural society, then the pedagogy it embodied should incorporate a hybridity of simulation scenarios (native, non-native and coethnic), enabling them to critically examine the impact of different types of identity they portray and negotiate their identities according to social constraints. This study suggests that in order to understand refugee integration more fully, it is not sufficient to account only for the pre-determined set of objective measures (such as employment and educational outcomes) without considering the way in which integration is actualized in everyday experience. For refugees in this study, what it means to be integrated into the Australian society was complex, ambivalent and context dependent. It was an ongoing process of contestation and negotiation between different values, identities and practices embedded in ethnic and mainstream Australia community. Based on my empirical study, I suggest that the everyday practices of refugee integration resembles with Bhabha’s (1990) notion of “Third Space” and incorporates the hybridity of cultural identifications and experiences

Screening of DUB activity and specificity by MALDI-TOF mass spectrometry

Deubiquitylases (DUBs) are key regulators of the ubiquitin system which cleave ubiquitin moieties from proteins and polyubiquitin chains. Several DUBs have been implicated in various diseases and are attractive drug targets. We have developed a sensitive and fast assay to quantify in vitro DUB enzyme activity using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Unlike other current assays, this method uses unmodified substrates, such as diubiquitin topoisomers. By analyzing 42 human DUBs against all diubiquitin topoisomers we provide an extensive characterization of DUB activity and specificity. Our results confirm the high specificity of many members of the OTU and JAMM DUB families and highlight that all USPs tested display low linkage selectivity. We also demonstrate that this assay can be deployed to assess the potency and specificity of DUB inhibitors by profiling 11 compounds against a panel of 32 DUBs

Ethanol reversal of tolerance to the respiratory depressant effects of morphine

Opioids are the most common drugs associated with unintentional drug overdose. Death results from respiratory depression. Prolonged use of opioids results in the development of tolerance but the degree of tolerance is thought to vary between different effects of the drugs. Many opioid addicts regularly consume alcohol (ethanol), and post-mortem analyses of opioid overdose deaths have revealed an inverse correlation between blood morphine and ethanol levels. In the present study, we determined whether ethanol reduced tolerance to the respiratory depressant effects of opioids. Mice were treated with opioids (morphine, methadone, or buprenorphine) for up to 6 days. Respiration was measured in freely moving animals breathing 5% CO(2) in air in plethysmograph chambers. Antinociception (analgesia) was measured as the latency to remove the tail from a thermal stimulus. Opioid tolerance was assessed by measuring the response to a challenge dose of morphine (10 mg/kg i.p.). Tolerance developed to the respiratory depressant effect of morphine but at a slower rate than tolerance to its antinociceptive effect. A low dose of ethanol (0.3 mg/kg) alone did not depress respiration but in prolonged morphine-treated animals respiratory depression was observed when ethanol was co-administered with the morphine challenge. Ethanol did not alter the brain levels of morphine. In contrast, in methadone- or buprenorphine-treated animals no respiratory depression was observed when ethanol was co-administered along with the morphine challenge. As heroin is converted to morphine in man, selective reversal of morphine tolerance by ethanol may be a contributory factor in heroin overdose deaths

Disease-specific survival for limited-stage small-cell lung cancer affected by statistical method of assessment

BACKGROUND: In general, prognosis and impact of prognostic/predictive factors are assessed with Kaplan-Meier plots and/or the Cox proportional hazard model. There might be substantive differences from the results using these models for the same patients, if different statistical methods were used, for example, Boag log-normal (cure-rate model), or log-normal survival analysis. METHODS: Cohort of 244 limited-stage small-cell lung cancer patients, were accrued between 1981 and 1998, and followed to the end of 2005. The endpoint was death with or from lung cancer, for disease-specific survival (DSS). DSS at 1-, 3- and 5-years, with 95% confidence limits, are reported for all patients using the Boag, Kaplan-Meier, Cox, and log-normal survival analysis methods. Factors with significant effects on DSS were identified with step-wise forward multivariate Cox and log-normal survival analyses. Then, DSS was ascertained for patients with specific characteristics defined by these factors. RESULTS: The median follow-up of those alive was 9.5 years. The lack of events after 1966 days precluded comparison after 5 years. DSS assessed by the four methods in the full cohort differed by 0–2% at 1 year, 0–12% at 3 years, and 0–1% at 5 years. Log-normal survival analysis indicated DSS of 38% at 3 years, 10–12% higher than with other methods; univariate 95% confidence limits were non-overlapping. Surgical resection, hemoglobin level, lymph node involvement, and superior vena cava (SVC) obstruction significantly impacted DSS. DSS assessed by the Cox and log-normal survival analysis methods for four clinical risk groups differed by 1–6% at 1 year, 15–26% at 3 years, and 0–12% at 5 years; multivariate 95% confidence limits were overlapping in all instances. CONCLUSION: Surgical resection, hemoglobin level, lymph node involvement, and superior vena cava (SVC) obstruction all significantly impacted DSS. Apparent DSS for patients was influenced by the statistical methods of assessment. This would be clinically relevant in the development or improvement of clinical management strategies

Perceptual Other-Race Training Reduces Implicit Racial Bias

Background: Implicit racial bias denotes socio-cognitive attitudes towards other-race groups that are exempt from conscious awareness. In parallel, other-race faces are more difficult to differentiate relative to own-race faces – the ‘‘Other-Race Effect.’ ’ To examine the relationship between these two biases, we trained Caucasian subjects to better individuate other-race faces and measured implicit racial bias for those faces both before and after training. Methodology/Principal Findings: Two groups of Caucasian subjects were exposed equally to the same African American faces in a training protocol run over 5 sessions. In the individuation condition, subjects learned to discriminate between African American faces. In the categorization condition, subjects learned to categorize faces as African American or not. For both conditions, both pre- and post-training we measured the Other-Race Effect using old-new recognition and implicit racial biases using a novel implicit social measure – the ‘‘Affective Lexical Priming Score’ ’ (ALPS). Subjects in the individuation condition, but not in the categorization condition, showed improved discrimination of African American faces with training. Concomitantly, subjects in the individuation condition, but not the categorization condition, showed a reduction in their ALPS. Critically, for the individuation condition only, the degree to which an individual subject’s ALPS decreased was significantly correlated with the degree of improvement that subject showed in their ability to differentiate African American faces