47 research outputs found
Solid-phase extraction and high-performance liquid chromatographic determination of polyphenols in apple musts and ciders
An improved analytical method was developed for the determination of polyphenols in the apple products must and cider. Phenolic compounds were fractionated into neutral and acidic groups by means of a solid-phase extraction method. The analytical method proposed was effective for the quantitation of phenolic compounds; recoveries between 84% and 111% were obtained, and the relative standard deviation was usually less than 5%
Phenolic Profile of Asturian (Spain) Natural Cider
The polyphenolic composition of natural ciders from the Asturian community (Spain), during 2
consecutive years, was analyzed by RP-HPLC and the photodiode-array detection system, without
previous extraction (direct injection). A total of 16 phenolic compounds (catechol, tyrosol, protocatechuic
acid, hydrocaffeic acid, chlorogenic acid, hydrocoumaric acid, ferulic acid, (-)-epicatechin,
(+)-catechin, procyanidins B2 and B5, phloretin-2¢-xyloglucoside, phloridzin, hyperin, avicularin, and
quercitrin) were identified and quantified. A fourth quercetin derivative, one dihydrochalcone-related
compound, two unknown procyanidins, three hydroxycinnamic derivatives, and two unknown
compounds were also found. Among the low-molecular-mass polyphenols analyzed, hydrocaffeic
acid was the most abundant compound, representing more than 80% of the total polyphenolic acids.
Procyanidins were the most important family among the flavonoid compounds. Discriminant analysis
was allowed to correctly classify more than 93% of the ciders, according to the harvest year; the
most discriminant variables were an unknown procyanidin and quercitrin