A forensic acquisition and analysis system for IaaS

Cloud computing is a promising next-generation computing paradigm that offers significant economic benefits to both commercial and public entities. Furthermore, cloud computing provides accessibility, simplicity, and portability for its customers. Due to the unique combination of characteristics that cloud computing introduces (including on-demand self-service, broad network access, resource pooling, rapid elasticity, and measured service), digital investigations face various technical, legal, and organizational challenges to keep up with current developments in the field of cloud computing. There are a wide variety of issues that need to be resolved in order to perform a proper digital investigation in the cloud environment. This paper examines the challenges in cloud forensics that are identified in the current research literature, alongside exploring the existing proposals and technical solutions addressed in the respective research. The open problems that need further effort are highlighted. As a result of the analysis of literature, it is found that it would be difficult, if not impossible, to perform an investigation and discovery in the cloud environment without relying on cloud service providers (CSPs). Therefore, dependence on the CSPs is ranked as the greatest challenge when investigators need to acquire evidence in a timely yet forensically sound manner from cloud systems. Thus, a fully independent model requires no intervention or cooperation from the cloud provider is proposed. This model provides a different approach to a forensic acquisition and analysis system (FAAS) in an Infrastructure as a Service model. FAAS seeks to provide a richer and more complete set of admissible evidences than what current CSPs provide, with no requirement for CSP involvement or modification to the CSP’s underlying architecture

Analysis of DNA ploidy and expression of tumour-associated antigens on human oral carcinomas xenografted in nude mice

Human squamous cell carcinomas (SCC) of the oral cavity were successfully established as xenografts in nude mice. Tumours with higher malignancy scores and involvement of lymph nodes in patients were more readily accepted as xenografts in nude mice. The xenografted tumours were characterised with respect to morphology, histology, DNA index and expression of tumour-associated antigens (TAA). Flow cytometric analysis of cellular DNA content revealed that many of the xenografts retained the parent tumour DNA pattern while some of the xenografts showed progression to aneuploidy. All the xenografted tumours expressed TAA recognised by monoclonal antibody (MAb) 3F8E3. On Western blotting, MAb 3F8E3 recognised proteins of molecular weight 62-64 kDa on parent and xenografted tumours. In general, the xenografts reflect many of the characteristics of the tumours from which they were derived and may provide a useful model for investigating newer approaches of treatment and diagnosis