3 research outputs found
Chondroitin sulfate immobilization at the surface of electrospun nanofiber meshes for cartilage regeneration
Aiming at improving the biocompatibility of biomaterial scaffolds, surface
modification presents a way to preserve their mechanical properties and to
improve the surface bioactivity. In this work, chondroitin sulfate (CS) was
immobilized at the surface of electrospun poly(caprolactone) nanofiber meshes
(PCL NFMs). The immobilization was performed with 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Contact Angle,
SEM, Optical Profilometry, FTIR, X-ray photoelectron spectroscopy techniques
confirmed the CS-immobilization in PCL NFMs. Furthermore, CS-immobilized
PCL NFMs showed lower roughness and higher hydrophilicity than the samples
without CS. Human articular chondrocytes (HACs) were cultured on electrospun
PCL NFMs with or without CS immobilization. It was observed that HACs
proliferated through the entire time course of the experiment in both types of
scaffolds. SEM observations revealed that HACs maintained their typical
morphology and produced extracellular matrix. Glycosaminoglycans
quantification showed increased values over time. Quantitative-PCR of
cartilage-related genes revealed over-expression of Aggrecan, Collagen type II,
COMP and Sox9 on both types of NFMs tested, with higher values for PCL. In
conclusion, CS immobilization in PCL NFM was achieved successfully and
provides a valid platform enabling further surface functionalization methods in
scaffolds to be developed for cartilage tissue engineering
Chondroitin sulfate immobilization at the surface of electrospun nanofiber meshes for cartilage tissue regeneration approaches
Aiming at improving the biocompatibility of biomaterial scaffolds, surface modification presents a way to preserve their mechanical properties and to improve the surface bioactivity. In this work, chondroitin sulfate (CS) was immobilized at the surface of electrospun poly(caprolactone) nanofiber meshes (PCL NFMs), previously functionalized by UV/O3 exposure and aminolysis. Contact angle, SEM, optical profilometry, FTIR, X-ray photoelectron spectroscopy techniques confirmed the success of CS-immobilization in PCL NFMs. Furthermore, CS-immobilized PCL NFMs showed lower roughness and higher hydrophilicity than the samples without CS. Human articular chondrocytes (hACs) were cultured on electrospun PCL NFMs with or without CS immobilization. It was observed that hACs proliferated through the entire time course of the experiment in both types of nanofibrous scaffolds, as well as for the production of glycosaminoglycans. Quantitative-PCR results demonstrated over-expression of cartilage-related genes such as Aggrecan, Collagen type II, COMP and Sox9 on both types of nanofibrous scaffolds. Morphological observations from SEM and LSCM revealed that hACs maintained their characteristic round shape and cellular agglomeration exclusively on PCL NFMs with CS immobilization. In conclusion, CS immobilization at the surface of PCL NFMs was achieved successfully and provides a valid platform enabling further surface functionalization methods in scaffolds to be developed for cartilage tissue engineering.Juliana F. Piai would like to acknowledge CAPES-Brazil and
CNPq-Brazil for the financial support (Proc. No. 201733/2009-9).
Marta Alves da Silva and Albino Martins would like to acknowledge
the Portuguese Foundation for Science and Technology (FCT),
FCT/MCTES (POCH − Programa Operacional Capital Humano, comparticipado
pelo Fundo Social Europeu e por fundos nacionais do
MCTES) for their post-doctoral grants (SFRH/BPD/73322/2010 and
SFRH/BPD/70669/2010). Authors would like to acknowledge the
patients of Hospital de Braga for the kind donation of biological
samples, as well as the medical and nursing staff, and CACTI-UVigo
(Spain) for the XPS and Optical Profilometry data analysis.info:eu-repo/semantics/publishedVersio