22 research outputs found
Neutrophils infected with highly virulent influenza H3N2 virus exhibit augmented early cell death and rapid induction of type I interferon signaling pathways
AbstractWe developed a model of influenza virus infection of neutrophils by inducing differentiation of the MPRO promyelocytic cell line. After 5days of differentiation, about 20–30% of mature neutrophils could be detected. Only a fraction of neutrophils were infected by highly virulent influenza (HVI) virus, but were unable to support active viral replication compared with MDCK cells. HVI infection of neutrophils augmented early and late apoptosis as indicated by annexin V and TUNEL assays. Comparison between the global transcriptomic responses of neutrophils to HVI and low virulent influenza (LVI) revealed that the IFN regulatory factor and IFN signaling pathways were the most significantly overrepresented pathways, with activation of related genes in HVI as early as 3h. Relatively consistent results were obtained by real-time RT-PCR of selected genes associated with the type I IFN pathway. Early after HVI infection, comparatively enhanced expression of apoptosis-related genes was also elicited
Seroepidemiological survey of Chlamydia pneumoniae infection among Singapore university undergraduates: Comparison between microimmunofluorescence and neutralization tests
10.1023/A:1013515200484World Journal of Microbiology and Biotechnology178773-778WJMB
Measurement of serum leptin concentrations in university undergraduates by competitive ELISA reveals correlations with body mass index and sex
American Journal of Physiology - Advances in Physiology Education271-470-77APED
Enterovirus and enterovirus infections.
Annals of the Academy of Medicine Singapore164683-68
A seroprevalence survey of dengue virus infection in healthy Singapore university undergraduates by enzyme immunoassay and plaque reduction neutralization test
Dengue Bulletin2997-10
Linoleic and linolelaidic acids differentially influence proliferation and apoptosis of molt-4 leukaemia cells
10.1006/cbir.2001.0733Cell Biology International258777-784CBII
Comparative Seroepidemiologic Analysis of Chlamydophila Pneumoniae Infection using Microimmunofluorescence, Enzyme Immunoassay and Neutralization Test: Implications for Serodiagnosis
10.1007/s12088-011-0168-4Indian Journal of Microbiology512223-229IJMB
Diagnosis of Nipah virus encephalitis by electron microscopy of cerebrospinal fluid
10.1016/S1386-6532(00)00094-9Journal of Clinical Virology193143-147JCVI
Identification of neutralizing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides
10.1016/j.virusres.2006.12.005Virus Research125161-6
Chlamydia pneumoniae IgG seropositivity and clinical history of ischemic heart disease in Singapore
Journal of Microbiology, Immunology and Infection363169-174JMII