176 research outputs found

    GEOCHEMICAL VARIATIONS ON HOSTED VOLCANIC ROCKS OF CIBALIUNG EPITHERMAL GOLD MINERALISATION, BANTEN – INDONESIA: IMPLICATIONS FOR DISTRIBUTION OF SUBDUCTION COMPONENTS

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    Subduction of the Indo-Australian Plate beneath the Eurasian Plate formed at least seven magmatic arcs in Indonesia. One of the magmatic arcs is the Neogene Sunda-Banda arc hosts various style of gold mineralisation such as Cibaliung epithermal gold mineralisation. Major and trace element data for host volcanic rocks to the Cibaliung epithermal gold mineralisation is provided by this study to identify the magmatic arc system and the distribution of subduction components. Enriched LILE (Large Ion Lithopile Element) and LREE (Light Rare Earth Element) compositions for basaltic andesite – rhyodacitic samples from the Cibaliung district are characteristic of calc-alkaline arcs. In this typical volcanic arc, the subduction component can be shown to make a dominant contribution to its content of LILE such as Rb, K, Th, and Ba enriched (more than 88%) relative to the mantle and within plate inputs. The incompatible elements (Hf, Zr, and Nb) cannot be observed in the subduction component and thus assumed to be derived from trace element enriched sub-continental lithosphere. These incompatible elements are defined as conservative elements therefore it suggests that the magma occurrence is related to a hydrous slab component. Keywords: Subduction, Indo-Australian plate, magmatic arcs, volcanic rocks, Cibaliung, epithermal gold

    Information Effect of Entry Into Credit Ratings Market: The Case of Insurers\u27 Ratings

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    The paper analyzes the effect of competition between credit rating agencies (CRAs) on the information content of ratings. We show that a monopolistic CRA pools sellers into multiple rating classes and has partial market coverage. This provides an opportunity for market entry. The entrant designs a rating scale distinct from that of the incumbent. It targets higher-than-average companies in each rating grade of the incumbent\u27s rating scale and employs more stringent rating standards. We use Standard and Poor\u27s (S&P) entry into the market for insurance ratings previously covered by a monopolist, A.M. Best, to empirically test the impact of entry on the information content of ratings. The empirical analysis reveals that S&P required higher standards to assign a rating similar to the one assigned by A.M. Best and that higher-than-average quality insurers in each rating category of A.M. Best chose to receive a second rating from S&P

    An improved method for surface immobilisation of RNA: application to small Non-Coding RNA - mRNA pairing

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    Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the interactions of bacterial small non-coding RNAs (sRNAs) with their mRNA targets are the subject of much current research. The technology of surface plasmon resonance (SPR) is an attractive approach to studying these interactions since it is highly sensitive, and allows interaction measurements to be recorded in real-time. Whilst a number of approaches exist to label RNAs for surface-immobilisation, the method documented here is simple, quick, efficient, and utilises the high-affinity streptavidin-biotin interaction. Specifically, we ligate a biotinylated nucleotide to the 3' end of RNA using T4 RNA ligase. Although this is a previously recognised approach, we have optimised the method by our discovery that the incorporation of four or more adenine nucleotides at the 3' end of the RNA (a poly-A-tail) is required in order to achieve high ligation efficiencies. We use this method within the context of investigating small non-coding RNA (sRNA)-mRNA interactions through the application of surface technologies, including quantitative SPR assays. We first focus on validating the method using the recently characterised Escherichia coli sRNA-mRNA pair, MicA-ompA, specifically demonstrating that the addition of the poly-A-tail to either RNA does not affect its subsequent binding interactions with partner molecules. We then apply this method to investigate the novel interactions of a Vibrio cholerae Qrr sRNA with partner mRNAs, hapR and vca0939; RNA-RNA pairings that are important in mediating pathogenic virulence. The calculated binding parameters allow insights to be drawn regarding sRNA-mRNA interaction mechanisms

    Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS

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    OxyS and RprA are two small noncoding RNAs (sRNAs) that modulate the expression of rpoS, encoding an alternative sigma factor that activates transcription of multiple Escherichia coli stress-response genes. While RprA activates rpoS for translation, OxyS down-regulates the transcript. Crucially, the RNA binding protein Hfq is required for both sRNAs to function, although the specific role played by Hfq remains unclear. We have investigated RprA and OxyS interactions with Hfq using biochemical and biophysical approaches. In particular, we have obtained the molecular envelopes of the Hfq–sRNA complexes using small-angle scattering methods, which reveal key molecular details. These data indicate that Hfq does not substantially change shape upon complex formation, whereas the sRNAs do. We link the impact of Hfq binding, and the sRNA structural changes induced, to transcript stability with respect to RNase E degradation. In light of these findings, we discuss the role of Hfq in the opposing regulatory functions played by RprA and OxyS in rpoS regulation

    2005-2006 Master Class - Zvi Zeitlin (Violin)

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    https://spiral.lynn.edu/conservatory_masterclasses/1142/thumbnail.jp

    2005-2006 Philharmonia Season Program Spring

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    Philharmonia No. 4 February 10, 2006 at 7:30 PM Jon Robertson, guest conductor ; Brandie Phillips, violins ; Vadim Makhovskiy, viola ; Anastasia Agapova, violin ; Ciprian Stancioi, clarinet Joy from Awakening (Songs of the Earth) / Joseph Curiale -- Poéme, op. 25 / Ernest Chausson -- Viola Concerto, op. posth / Béla Bartók -- Violin Concerto No. 1 in D Major, op. 19 / Sergey Prokofiev -- Concertino in E-flat Major for Clarinet and Orchestra, op. 26 / Carl Maria von Weber Philharmonia No. 5 March 31, 2006 at 7:30 PM Albert-George Schram, conductor and music director Variations on America, Charles Ives -- West Side Story: Symphonic Dances / Leonard Bernstein -- Merry Mount Suite / Howard Hanson -- Symphony No. 1 in One Movement, op. 9 / Samuel Barber Philharmonia No. 6 April 21, 2006 at 7:30 PM Albert-George Schram, conductor and music director Symphony No. 40 in G Minor, K. 550 / Wolfgang Amadeus Mozart -- Symphony No. 2 in E Minor, op. 27 / Sergei Rachmaninoffhttps://spiral.lynn.edu/conservatory_philharmonia/1033/thumbnail.jp

    Whole genome sequencing of Salmonella Typhimurium illuminates distinct outbreaks caused by an endemic multi-locus variable number tandem repeat analysis type in Australia, 2014

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    Phylogeny of the outbreak A and M strains in the context of national and international STM isolates. Genome data analysed in Octavia et al. representing five STM outbreaks in Australia [25]; Kingsley et al. representing ST313 outbreak in Malawi [30]; Leekitcharoenphon et al. representing six STM outbreaks in Denmark [15] and Hawkey et al. representing STM DT135a outbreak in Australia [21] were also included as comparisons and marked as the corresponding study/outbreak. Other branches that are not labelled are background isolates from the above studies; draft genomes from Pang et al. [29] which include five diverse Australian STM isolates; Fu et al. representing Salmonella reference collection A; [28] and other fully sequenced STM genomes available from GenBank including LT2 (Accession No. NC003197), 798 (Accession No. CP003386), DT2 (Accession No. HG326213), DT104 (Accession No. HF937208), 14028S (Accession No. CP001363), SL1344 (Accession No. FQ312003), UK-1 (Accession No. CP002614), T000240 (Accession No. AP011957), U288 (Accession No. CP003836) and ST4/74 (Accession No. CP002487). Bootstrap values if greater than 50 %, are presented on the internal branches. (PPTX 74 kb

    High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions

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    We are just beginning to unravel the myriad of interactions in which non-coding RNAs participate. The intricate RNA interactome is the foundation of many biological processes, including bacterial virulence and human disease, and represents unexploited resources for the development of potential therapeutic interventions. However, identifying specific associations of a given RNA from the multitude of possible binding partners within the cell requires robust high-throughput systems for their rapid screening. Here, we present the first demonstration of functional-RNA arrays as a novel platform technology designed for the study of such interactions using immobilized, active RNAs. We have generated high-density RNA arrays by an innovative method involving surface-capture of in vitro transcribed RNAs. This approach has significant advantages over existing technologies, particularly in its versatility in regards to binding partner character. Indeed, proof-of-principle application of RNA arrays to both RNA-small molecule and RNA-RNA pairings is demonstrated, highlighting their potential as a platform technology for mapping RNA-based networks and for pharmaceutical screening. Furthermore, the simplicity of the method supports greater user-accessibility over currently available technologies. We anticipate that functional-RNA arrays will find broad utility in the expanding field of RNA characterization
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