Evaluation of early stage human bone marrow stromal proliferation, cell migration and osteogenic differentiation on μ-MIM structured stainless steel surfaces

It is well established that surface topography greatly affect cell—surface interactions. In a recent study we showed that microstructured stainless steel surfaces characterized by the presence of defined hexagonally arranged hemisphere-like structures significantly affected cell architecture (shape and focal adhesion size) of primary human bone mesenchymal stromal cells. This study aimed at further investigating the influence these microstructures (microcline protruding hemispheres) on critical aspects of cell behaviour namely; proliferation, migration and osteogenic differentiation. As with previously reported data, we used primary human bone mesenchymal stromal cells to investigate such effects at an early stage in vitro. Cells of different patients were utilised for cell migration studies. Our data showed that an increase in cell proliferation was exhibited as a function of surface topography (hemispheres). Cell migration velocity also varied as a function of surface topography on patient specific basis and seems to relate to the differentiated state of the seeded cell population (as demonstrated by bALP positivity). Osteogenic differentiation, however, did not exhibit significant variations (both up and down-regulation) as a function of both surface topography and time in cultur

Disentangled UHMWPE@silica powders for potential use in power bed fusion based additive manufacturing

Disentangled ultrahigh molecular weight polyethylene dUHMWPE (Mw ∼ 2.106 Da) particles in a reactor blend with HDPE are catalytically prepared from ethylene, mediated by a new catalyst from N,N'-(2,6-pyridinediyl diethylidyne) bis[2,6-di-3-propenyl-benzenamine] iron dichloride and triethyl aluminum. These particles could be laser sintered, but not automatically processed in an SLS machine. The same catalyst supported on microsilica particles gives access to composite dUHMWPE@silica particle powder with particle sizes below 200 µm. Testing bars prepared by heat pressing have an Emod of 150 MPa, an elongation at break at 450 % and an ultimate strength of 39 ± 11 MPa. A SEM image indicates a silica induced crystallization into pseudo spherulites of 500 µm size. The dUHMWPE@silica composite particles have an fcc flowability value of 3.4 in a ring shear tester, and a low density of 150 kg.m−3. Additivation with nanosilica powder (1 wt%) and carbon black (0.25 wt%) allowed to process the composite in an SLS machine. The printed parts showed severe caking, but also a complete welding of the powder, albeit with voids on account of the low particle density

Piston-Based Material Extrusion of Ti-6Al-4V Feedstock for Complementary Use in Metal Injection Molding

Piston-based material extrusion enables cost savings for metal injection molding users when it is utilized as a complementary shaping process for green parts in small batch sizes. This, however, requires the use of series feedstock and the production of sufficiently dense green parts in order to ensure metal injection molding-like material properties. In this paper, a methodological approach is presented to identify material-specific process parameters for an industrially used Ti-6Al-4V metal injection molding feedstock based on the extrusion force. It was found that for an optimum extrusion temperature of 95 °C and printing speed of 8 mm/s an extrusion force of 1300 N ensures high-density green parts without under-extrusion. The resulting sintered part properties exhibit values comparable to metal injection molding in terms of part density (max. 99.1%) and tensile properties (max. yield strength: 933 MPa, max. ultimate tensile strength: 1000 MPa, max. elongation at break: 18.5%) depending on the selected build orientation. Thus, a complementary use could be demonstrated in principle for the Ti-6Al-4V feedstock

Evaluation of the osteogenic differentiation of human mesenchymal stem cells on modified bone substitute materials

Introduction: The development and improvement of new materials for bone tissue engineering is desired due to the restricted availability of autologous material and improvable material characteristics of available alloplastic materials1. To test the potential of newly designed materials and/or modifications, the adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs) can be used to perform in vitro studies. In this study we evaluated the adhesion velocity of hMSCs on different modified materials as titan, iron composites and polystyrene to estimate the modification strategy. Materials and Methods: hMSCs were isolated of bone marrow and were characterized thoroughly by FACS analysis, histochemical staining and qRT-PCR studies concerning their osteogenic differentiation potential. Cell adhesion on the different modified materials was evaluated by immunological staining for actin and vinculin at different time points. The osteogenic differentiation of the cells on selected modifications was detected by quantitative measurements of the alkaline phosphatase as well as determining the total DNA volume of adherent cells. Results and Discussion / References: Modified surfaces revealed an influence on cell adhesion. An increase of adherent cells was detected after 24 hours and 48 hours of cell culture. Furthermore, an increase of ALP was seen in differentiated cells after 14 days of osteogenic induction. The results of this study indicate the importance of appropriate surface characteristics for cell adhesion and differentiation. In vitro testing of newly developed materials/surfaces is an important tool to reduce animal testing and must be further developed to acheive reproducible and significant test methods with primary cells