Distributions of Individual Domains of the EQ-5D-3L and the ICECAP-O.

<p>Distributions of Individual Domains of the EQ-5D-3L and the ICECAP-O.</p

Characteristics of the VCI participants at baseline.

<p>Characteristics of the VCI participants at baseline.</p

Kappa.

<p>Kappa.</p

Genogroup Distribution and Meningococcal Identification of the 234 Carriage Isolates by Real-Time PCR.

<p>n.a. = not applicable; NG = nongroupable by SASG assay; NGG = nongenogroupable by rt-PCR assays; UKN = unknown (no result by genogrouping rt-PCR assays and <i>cnl</i> PCR negative or not done).</p><p>^aPositive result with two rt-PCR genogrouping assays (Ct<30).</p><p>Genogroup Distribution and Meningococcal Identification of the 234 Carriage Isolates by Real-Time PCR.</p

Diversity of the fHbp variants in the carriage isolate collection.

<p>Distribution of fHbp variants found in three or more isolates. Fourteen fHbp variants encompassed 206 (88%) isolates in the collection. Blue bars, fHbp subfamily A variants; red bars, fHbp subfamily B variants.</p

Location of serogroup-specific PCR amplicons in capsular biosynthesis genes of <i>N</i>. <i>meningitidis</i> serogroups A, B, C, W, Y, E, Z, and X.

<p>Genes <i>ctrA-D</i> encode capsule transport proteins conserved among all eight serogroups. Genes <i>cssA-C</i> are responsible for the sialic acid capsular component of serogroups B, C, W, and Y, while <i>csb-c-w-y</i> encode the sialyltransferases that produce the serogroup-specific linkages of sialic acid with glucose or galactose. Capsule biosynthesis genes <i>csaA-D</i>, <i>csxA-C</i>, <i>cseA-G</i>, and <i>cszA-D</i>, are unique to A, X, E, and Z serogroups, respectively. Polysaccharide transport and biosynthesis genes are flanked by <i>tex</i> and <i>galE</i> (black arrows). Genes at the distal end of the biosynthetic clusters in serogroups B, C, W, and Y are shown as dotted arrows, and annotated as described previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132140#pone.0132140.ref035" target="_blank">35</a>]. The green arrows indicate the location of the <i>ctrA</i> primers used to detect all eight serogroups. Light blue arrows represent the initial primers designed to identify NmE and NmZ, based on sequence at the 5’ end of the <i>ctrA</i> gene. The red arrows represent the position of the final primers designed for the eight serogroup-specific assays. The blow-up at the bottom illustrates the position of <i>ctrA</i> primers and probes in greater detail.</p

Results of serological testing of specimens from 2013 Florida patient with anthrax-like cutaneous lesion.

<p>Results of serological testing of specimens from 2013 Florida patient with anthrax-like cutaneous lesion.</p

<i>B</i>. <i>cereus</i> BcFL2013 capsule. India ink stain of <i>B</i>. <i>cereus</i> BcFL2013 after overnight growth in defibrinated horse blood.

<p><i>B</i>. <i>cereus</i> BcFL2013 capsule. India ink stain of <i>B</i>. <i>cereus</i> BcFL2013 after overnight growth in defibrinated horse blood.</p

Anthrax-like cutaneous lesion on left check of 70-year-old male Florida resident.

<p>Anthrax-like cutaneous lesion on left check of 70-year-old male Florida resident.</p

Summary of results for testing of <i>B</i>. <i>cereus</i> BcFL2013 compared to <i>B</i>. <i>cereus</i> G9241 and <i>B</i>. <i>anthracis</i> Ames.

<p>Summary of results for testing of <i>B</i>. <i>cereus</i> BcFL2013 compared to <i>B</i>. <i>cereus</i> G9241 and <i>B</i>. <i>anthracis</i> Ames.</p