Net Enclosure of ‘Honeycrisp’ and ‘Gala’ Apple Trees at Different Bloom Stages Affects Fruit Set and Alters Seed Production

Thinning is a critical but challenging practice in apple production, especially for organic producers. The objective of this experiment was to determine if exclusion netting, used to manage insect pests and stress, could reduce fruit set and thinning requirements of ‘Gala’ and ‘Honeycrisp’ apple trees in Michigan and Washington, U.S.A., respectively. Nets were closed around whole canopies at different timings based on a predetermined percentage of open flowers. In 2017 and 2018, netted trees set a full commercial crop regardless of percentage of open bloom at the time of netting, including prebloom enclosures. Fruit set and yield of netted ‘Honeycrisp’ trees were significantly lower than non-netted, nonthinned controls but similar to non-netted hand-thinned controls. ‘Gala’ fruit set and yield did not differ among treatments. Exclusion netting markedly reduced the number of mature seeds and increased the number of nonfertilized seeds in both cultivars. Pollinator exclusion to ‘Gala’ in a frost year increased parthenocarpic fruit set two-fold compared to non-netted trees. Fruit size, shape, and quality attributes of ‘Gala’ were were similar among treatments, but ‘Honeycrisp’ fruit were significantly smaller than hand-thinned, non-netted controls. Netting may constitute an alternative, viable strategy to manage fruit set but requires testing on different cultivars

Genetic mechanisms associated with floral initiation and the repressive effect of fruit on flowering in apple (Malus x domestica Borkh).

Many apple cultivars are subject to biennial fluctuations in flowering and fruiting. It is believed that this phenomenon is caused by a repressive effect of developing fruit on the initiation of flowers in the apex of proximal bourse shoots. However, the genetic pathways of floral initiation are incompletely described in apple, and the biological nature of floral repression by fruit is currently unknown. In this study, we characterized the transcriptional landscape of bourse shoot apices in the biennial cultivar, 'Honeycrisp', during the period of floral initiation, in trees bearing a high fruit load and in trees without fruit. Trees with high fruit load produced almost exclusively vegetative growth in the subsequent year, whereas the trees without fruit produced flowers on the majority of the potential flowering nodes. Using RNA-based sequence data, we documented gene expression at high resolution, identifying >11,000 transcripts that had not been previously annotated, and characterized expression profiles associated with vegetative growth and flowering. We also conducted a census of genes related to known flowering genes, organized the phylogenetic and syntenic relationships of these genes, and compared expression among homeologs. Several genes closely related to AP1, FT, FUL, LFY, and SPLs were more strongly expressed in apices from non-bearing, floral-determined trees, consistent with their presumed floral-promotive roles. In contrast, a homolog of TFL1 exhibited strong and persistent up-regulation only in apices from bearing, vegetative-determined trees, suggesting a role in floral repression. Additionally, we identified four GIBBERELLIC ACID (GA) 2 OXIDASE genes that were expressed to relatively high levels in apices from bearing trees. These results define the flowering-related transcriptional landscape in apple, and strongly support previous studies implicating both gibberellins and TFL1 as key components in repression of flowering by fruit