Biochemical Characterization of Diamide Inhibitors with N-acetylglucosaminidases LytG from Bacillus subtilis

In recent years the frequency of antibiotic resistance has been on the rise creating a need for antibiotic development with specific and lethal targets. It has been recently reported that glycosyl trizole are a novel class of antibacterial agents (1). Further investigation on the antibacterial ability of glycosyl triazole inhibitors has shown that targets include exo-acting N-acetylglucosaminidases (GlcNAcase) LytG (Bacillus subtilis) and FlgJ (Salmonella enterica) of the GH73 family (2). The Glycoside Hydrolase Family 73 (GH73) is characterized by bacterial and viral glycoside hydrolase. This enzyme cleaves the β-1,4-glycosidic linkage between N-acetylglucosaminyl (NAG) and N-acetylmuramyl (NAM) of the carbohydrate backbone in bacterial peptidoglycan. Glycoside hydrolase can occur as an endo- or exo- process, depending on the region of the chain that is cleaved. Endo-acting refers to activity in the middle of the chain, whereas exo-acting refers to the ends (typically the non-reducing end) (3). Currently, there is no kinetic parameters that have been determined for any member of the GH73 family, however binding and kinetic characterization will be performed for select glycosyl triazole inhibitors and GH73 targets interactions. Further studies will involve crystallization and GlcNAcase activity assays to identify GH73 family members as the target of glycosyl triazole inhibitors. Through these studies the interaction between the non-competing inhibitor and the GH73 target will be characterized. Additionally, it will be demonstrated that these Ugi- derived compounds are competitive inhibitors of GH73 enzymes

The Phillips Machine (MONIAC)

The Phillips Machine, or Monetary National Income Analogue Computer (MONIAC), is a hydraulic representation of cash flow within the UK economy in the early 20th century. It represents the circular flow of income (New Zealand Institute of Economic Research, 2015), shown by the economic equation Aggregate Demand = Consumer Expenditure + Investment + Government Spending + (Exports – Imports), or Y = C + I + G + (X – M), which is an important equation in determining the national output of an economy (Pettinger, 2008). Though no longer in use, of the 14 that were built, the majority were originally restricted to military and government use owing to their effectiveness in determining economic policy. This paper describes an implementation of an emulation of this machine that satisfies, or mostly satisfies, almost all of the requirements specified in the ISO/IEC 25010 software quality assurance standard and would be suitable for deployment to support A-Level Economics teaching. With further work it would also become suitable as part of a museum display of a Phillips Machine

Legal interpreters in the news in Ireland

This article consists of a review of court reports from national and provincial newspapers in Ireland from 2003 to 1st August 2010. The reports provide an insight into the attitudes of judges, lawyers and police officers to defendants who are not proficient in English. The issue of defendants’ proficiency in English is a recurrent one. Coverage suggests that interpreters are not always provided in police stations or in the courts and that some judges continue to allow friends and family members to act as interpreters. Meanwhile, some solicitors consistently request interpreters for their clients. Other salient issues are cost, interpreter competency and interpreter ethics

Interpreting in Northern Ireland

This article examines how interpreter provision in Northern Ireland developed in a very different way from Ireland or indeed England, Scotland or Wales. In general terms, interpreter provision in Northern Ireland is very good in that interpreters are routinely provided for hospitals, social welfare, schools and of course police stations and courts. The majority of interpreters have undergone training, and instead of outsourcing interpreting services to a translation agency, the authorities have opted for an in-house service for health and social welfare, a social economy enterprise for legal interpreting and a community development organisation for other types of interpreting. Each organisation has a register of interpreters

Army Supplies in the Forward Area and the Tumpline System: A First World War Canadian Logistical Innovation

Editor’s Note: In British Logistics on the Western Front, 1914–1919 (Praeger, 1998), Ian M. Brown documents the problems of maintaining an army in the field; throughout that war, supply lines were strained to get food, equipment and ammunition forward. Early problems of adequate supplies were replaced by an inability to get the items from depots to where they were required. Some of these latter problems were blamed on “establishments” and other force-structure problems caused by stripping logistical-support units to meet the manpower needs of the fighting units. The decision to dramatically reduce the size of the BEF divisions in France helped reduced pressures. By stripping a battalion out of each brigade, and using the men freed as replacements, the BEF maintained its paper strength in divisions (though, in fact, the strength went considerably down) and, more importantly, also reduced its overall logistical requirements. But, as Brown writes: The Canadian Corps successfully resisted this “downsizing” as its commander opposed the reduction vehemently. In fact, he managed to increase the effective size and strength of his divisions by using the manpower from the two [sic] Canadian divisions forming in Britain. This gave Haig a single very strong corps—four overstrength divisions amounting to some one hundred thousand men (forty-eight thousand infantry)—but it also gave his administration a supply problem, since the standard divisional pack could not supply a Canadian division. In spite of this trouble, it did not appear to cause great difficulty on the lines of communication. Indeed, it gets no mention in either the QMG’s or AG’s diaries.... (pp.165-166) Buried in this passage lay two secrets. The four-battalion brigades perhaps (too simply?) explain the use of the Canadian Corps as Haig’s “shock troops.” But as Brown notes, how the Canadians maintained these larger formations is not clear from British sources (p. 177). The answer to this secret must be sought elswhere. One answer in F.R. Phelan’s “tumpline.

Does the European Union Strengthen the State? Democracy, Executive Power and International Cooperation. CES Working Paper, no. 95, 2003

Many observers have suggested that the strengthening of executives vis-à-vis other political actors, in particular national parliaments, has been one of the principal effects of European integration (and perhaps international cooperation more generally) on national democracies, with democracy being “perverted” and parliaments becoming “rubber stamps.” Moravcsik (1994) has argued that there were four theoretical ways in which international cooperation could “strengthen the state,” by redistributing institutional power, initiative (agenda-setting), information and ideas in favor of the executive in Europe. However, consideration of domestic politics in Europe shows that elites are already – for exogenous reasons – dominant in institutions, initiative and information. In Europe, therefore, the “strong” executive is not a product of European integration, and the reverse may even be true: that the dominance of the executive in national political systems has been a prerequisite for the success of European integration – that European openness has been built on national political closure. More generally, assessments of the impact of international cooperation on democracy should measure the effect of international cooperation at the margin on the existing characteristics of particular national democratic systems

Cruise Report 70-S-7: Big Game Fisheries Investigations

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Democracy, the academic field and the (New Zealand) journalistic habitus

The relationship between journalism and the academy is historically fraught. Any mention of the word ‘theory’ is only likely to exacerbate these tensions, since it perhaps signifies, most clearly, the division between both identities. Drawing on the social theory of Pierre Bourdieu, this paper considers, with particular empirical reference to the New Zealand context, the often antagonistic relationship between the ‘journalistic field’ and the ‘academic field’. I examine how academic identities are sometimes represented ‘fantasmatically’ (Glynos and Howarth, 2007) in journalistic discourse and explore the contradictions between journalism’s official commitment to democratic values and the desire of at least some journalists to silence or lampoon academic voices, or insist that theoretical reflection is somehow incompatible with good journalism. The articulation of particular journalistic identities is contextualised with reference to the more ‘objective’ logic of the New Zealand journalistic field and, in particular, the structuring of its concrete relationship with the academic field through journalism education programmes. Although the culturally sedimented practices precluding the possibility of a different inter-field dynamic are considerable, I conclude by ‘visualising’ an alternative relationship, one constituted, on all sides, by what Williams Connolly (2005) characterises as a properly democratic ethos of ‘agonistic respect’ across difference

Medical interpreting and the law in the European Union

In 2011, the Danish government announced that from June that year it would no longer cover the costs of medical interpreters for patients who had been living in Denmark for more than seven years. The Dutch Ministry of Health followed with an even more draconian approach; from 1 January 2012 the cost of translation and interpreting would no longer be covered by the state. These two announcements led to widespread concern about whether or not there is a legal foundation for interpreter provision in healthcare. This article considers United Nations treaties, conventions from the Council of Europe and European Union law. European Union member states have been slow to sign up to international agreements to protect the rights of migrant workers. The European Union itself has only recently moved into the area of discrimination and it is unclear if the Race Directive covers language. As a result, access to interpreters in healthcare, where it exists, is dependent on national anti-discrimination legislation or on positive action taken at national or local level rather than on European or international law

Cruise Report 71-KB-16: Big game fish investigation

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