Equivariant Crossed Modules and Cohomology of Groups with Operators

In this paper we study equivariant crossed modules in its link with strict graded categorical groups. The resulting Schreier theory for equivariant group extensions of the type of an equivariant crossed module generalizes both the theory of group extensions of the type of a crossed module and the one of equivariant group extensions.Comment: 20 pag

EMPIRICAL LIKELIHOOD, CLASSIFICATION AND APPROXIMATE BAYESIAN COMPUTATION

Ph.DDOCTOR OF PHILOSOPH

Crossed bimodules over rings and Shukla cohomology

In this paper we present some applications of Ann-category theory to classification of crossed bimodules over rings, classification of ring extensions of the type of a crossed bimodule

SCREENING, IDENTIFICATION AND BIOACTIVE PROPERITES OF BACILLUS SPP. ISOLATED FROM SHRIMP PONDS OF QUANG TRI PROVINCE

Aquaculture is the farming of aquatic organisms by intervention in the rearing process to enhance production and private ownership of the stock being cultivated. However, the intensification of aquaculture practices requires cultivation at high densities, which has caused significant damage to the environment and , aquatic species are subjected to high-stress conditions, increasing the incidence of diseases and causing a decrease in productivity. This study was conducted to isolate bacteria from sediments of shrimp culture ponds based on antagonistic activity against shrimp pathogen, Vibrio parahaemolyticus, and production of exocellular enzymes, protease and amylase. Three potential strains were isolated and after analysis of 16S rRNA gene, the isolated starin were identified to Bacillus subtilis (isolate QTA12 with amylase activity), Bacillus megaterium (QTP1 with proteolytic activity ) and Bacillus subtilis (isolate with antivibrio activity DK1). These isolated strains may be considered for future using in shrimp culture ponds as a very promising measure for sustainable aquaculture

Screening genes encoding protein protease inhibitor from metagenome of sponge-associated microorganisms in Quang Tri sea, Vietnam

Using metagenomics-based method to isolate new compounds from the marine environment are getting more and more attention in recent years. Based on metagenome library, bioinformatics methods is a powerful tool for screening genes with new biological activities from uncultured microorganisms and become a breakthrough in research and application of biotechnology. In this study we selected and used the samples DNA QT2 which had high DNA content and purity from a total of 6 DNA samples of sponge-associated microorganisms collected in Quang Tri sea (Vietnam) for metagenomic sequencing (DNA concentration is 202.5 ng, A260/ A280 value is 1.80). 16S rRNA metagenomic sequencing data of QT2 produced 44,117,722 reads, which were assembled into 120,236 contigs. ORF prediction using Prodigal produced 386,416 ORFs. Functional annotation was conducted based on 7 different databases (NR, COG, CAZy, Swissprot, GO, KEGG, Pfam), and there are 266,553 genes were annotated using Swiss-Prot. In addition, based on the obtained metagenomic data, 50 complete genes encoding protease inhibitor proteins were revealed and among them, 28 genes encoding protein ( 50%) belonged to the serine protease inhibitor family, and 22 genes genes encoding belonged to the Inter-alpha-trypsin inhibitor group. NCBI BLAST screening results that these proteins had higher 50% identity to protease inhibitors.

Environmentally Responsible Bioengineering for Spore Surface Expression of Helicobacter pylori Antigen

The development of genetic technologies and bioengineering are creating an increasing number of genetically engineered microorganisms with new traits for diverse industrial applications such as vaccines, drugs and pollutant degraders. However, the destiny of genetically engineered bacterial spores released into the environment as long-life organisms has remained a big environmental challenge. In this study, an environmentally responsible and sustainable gene technology solution based on the concept of thymine starvation is successfully applied for cloning and expression of a Helicobacter pylori antigen on Bacillus subtilis spore surface. As an example, a recombinant Bacillus subtilis strain A1.13 has been created from a gene fusion of the corresponding N-terminal fragment of spore coat protein CotB in B. subtilis and the entire urease subunit A (UreA) in H. pylori and the fusion showed a high stability of spore surface expression. The outcomes can open the door for developing highly safe spore vectored vaccines against this kind of pathogen and contributing to reduced potential risks of genetically engineered microorganisms released in the environment

Assesment of antidermatophyte activity of oil from Curcuma longa L. in vitro

Curcuma longa L. is a rhizomatous herbaceous perennial plant, and its essential oil showed antifungal, antibacterial, antioxydant, antivenom, and antitumor effects. The antidermatophytes of oil from turmeric was demonstrated. This study was carried out in order to evaluate inhibition activity of oil from Vietnamese turmeric against two strains dermatophyte, Trychophytol mentargrohytes and Candida albicans. The oil from Vietnamese turmeric was obtained by hydrodistillation method and its chemical composition was determined by GC/MS. By comparing with authentic reference compounds in mass spectra library Nist 98.1 and Wiley 275.L, 13 compounds of oil from Vietnamese turmeric were identified. The received results showed that, the oil is rich in turmerones (about 44%), which has been recognized as major constituent attributing to antimicrobial activity. By disc diffusion method, the antifungal activity of the turmeric essential oil against T. mentargrophyte and C. albicans was demonstrated. The MICs of the turmeric oil for T. mentargrophytes và C. albicans were 0.5%. The minimal killing time for C. albicans was 60 minutes and for T. mentargrophyte was 90 minutes after treatment with the turmeric oil