Recombinant Streptodornase Production in Escherichia .coli 1

Abstract: Introduction: In pyoderma infections, pus of density related to desoxiribonucleoproteins. streptodornase(sd) combination with streptokinase dissolve purulent secretions from infections.This study aims to produce recombinant streptodornase of Streptococcus pyogenes in E.coli. Methods: by using specific primers streptodornase gene (sd) was amplified. The constructed vector pGEX4T1-sd was transformed in E. coli BL21 (DE3)/ pLYS-S and induced with IPTG. Recombinant protein was purified by affinity-chromatography (Glutathione Sepharose 4B). Results: a PCR product about 816 bp was seen. Sd recombinant protein was expressed in pGEX-4T-1 in E. coli BL21. SDS-PAGE analysis of product before and after inducing indicated a band about 60 kDs. A High concentration of the recombinant protein obtained from the single-step purification by affinity-chromatography (Glutathione Sepharose 4B), and purified recombinant Sd protein appeared at about 38.2 kDa. Conclusion: results showed Sd recombinant protein has antigenic characteristics and it can be used for diagnostic aims