Phân lập và xác định một số đặc điểm sinh học của vi khuẩn phân giải cellulose từ đất trồng sâm Ngọc Linh tại tỉnh Quảng Nam

Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) is an endemic species in Vietnam and was discovered at the Ngoc Linh mountain (Kon Tum/Quảng Nam). Investigations showed that the soil with a thick layer of humus was the ideal condition for growth and development of Ngoc Linh ginseng. Therefore research on microbial flora as well as cellulose-degrading bacteria in ginseng soil may elucidate factors contributing to acclimatized cultivation of this ginseng in Vietnam. From the soil sample with cultivated Ngoc Linh ginseng in Quang Nam, five bacteria strains with cellulose-degrading activities were isolated (QN1, QN2, QN3, QN4, QN5 with respectively hydrolyzed CMC halos diameters of 10, 11, 22, 7, 22 mm) with cellulase activities of 1,31; 1,23; 2,99; 0,99; 2,51 U/ml. The combination of 16S rRNA gene sequences and cultured/biochemical characteristics of the bacteria showed that the five bacteria strains was classified to be Pseudomonas sp. QN1; Pseudomonas sp. QN4; Bacillus sp. QN2; Bacillus sp. QN3; Roseomonas sp. QN5.Sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.) là loài đặc hữu của Việt Nam được phát hiện tại vùng núi Ngọc Linh (Kon Tum/Quảng Nam). Kết quả khảo sát cho thấy những vùng đất có tầng mùn dày là nơi có điều kiện lý tưởng cho cây sâm Ngọc Linh sinh trưởng và phát triển. Chính vì vậy việc tìm hiểu về khu hệ vi sinh vật nói chung, vi khuẩn phân giải cellulose nói riêng trong đất trồng sâm là một trong những hướng đi tiềm năng cho nghiên cứu trồng di thực loài sâm này của Việt Nam. Từ mẫu đất trồng sâm Ngọc Linh tại Quảng Nam, chúng tôi đã phân lập được 05 vi khuẩn có hoạt tính phân giải cellulose (kí hiệu QN1, QN2, QN3, QN4, QN5 với kích thước vòng phân giải đo được trên môi trường chứa cơ chất CMC (0,1%) tương ứng đạt: 10, 11, 22, 7, 22 mm), hoạt tính cellulase đạt lần lượt là 1,31; 1,23; 2,99; 0,99; 2,51 U/ml. Kết hợp nghiên cứu một số đặc điểm nuôi cấy/sinh hóa và phân tích trình tự gen 16S rARN đã xác định vị trí phân loại của các vi khuẩn phân lập được như sau: QN1 và QN4 thuộc chi Pseudomonas; QN2, QN3 thuộc chi Bacillus; QN5 thuộc chi Roseomonas

APPLICATION OF SHIFTING ASSAY OF STANDARDIZED INDIRECT AGGLUTINATION (SSIA) FOR DETECTION OF ANTIGENS OF NEWCASTLE DISEASE AND INFECTIOUS BURSAL DISEASE VIRUSES IN CHICKEN FAECES

. Using the method of shifting assay of standardized indirect agglutination (SSIA), the prevalence of Newcastle disease viruses (NDV) and infectious bursal disease viruses (IBDV) in chickens reared in several districts of Thua Thien Hue province in the Spring-Summer and Fall-Winter seasons was determined. In the Spring-Summer season of 2011, about 22.3% of the chickens were infected with NDV, in which A Luoi  accounted for the highest percentage of 25% of the infected chickens and Huong Thuy  the lowest  of 18.2%. Meanwhile, 36% of the same chickens were infected with IBDV, with the highest percentage (46.66%) also in A Luoi and the lowest (30.3%) also in Huong Thuy. The intensity of NDV infection in the Spring-Summer season in A Luoi and Phu Vang was highest (GMT = 1.45), and in Huong Thuy lowest (GMT = 1.31). In addition, in the Fall-Winter season, about 46% of the chickens were infected with NDV and 46.3% with IBDV in Huong Thuy and Phu Vang – two neighbouring districts of Hue City, in which NDV was detected in 54.4% of the chickens in Huong Thuy and 33.9% in Phu Vang. In contrast, IBDV was detected in 41.9% and 52.7% of the chickens respectively in the two districts. The infection was not inter-dependent. Methodically, although the differences in the infection rates were insignificant with the accuracy of 95%, faecal samples showed higher sensitivity in SSIA analyses for both cases of NDV and IBDV infection in comparision with mouth exudates. By SSIA method, results could be read clearly with unaided eyes for a long time after the performance, and it was also proven applicable for cases of haemagglutinating viruses if proper treatments for depletion of animal RBCs’ surface agglutinins could be applied

APPLICATION OF SHIFTING ASSAY OF STANDARDIZED INDIRECT AGGLUTINATION (SSIA) FOR DETECTION OF ANTIGENS OF NEWCASTLE DISEASE AND INFECTIOUS BURSAL DISEASE VIRUSES IN CHICKEN FAECES

. Using the method of shifting assay of standardized indirect agglutination (SSIA), the prevalence of Newcastle disease viruses (NDV) and infectious bursal disease viruses (IBDV) in chickens reared in several districts of Thua Thien Hue province in the Spring-Summer and Fall-Winter seasons was determined. In the Spring-Summer season of 2011, about 22.3% of the chickens were infected with NDV, in which A Luoi  accounted for the highest percentage of 25% of the infected chickens and Huong Thuy  the lowest  of 18.2%. Meanwhile, 36% of the same chickens were infected with IBDV, with the highest percentage (46.66%) also in A Luoi and the lowest (30.3%) also in Huong Thuy. The intensity of NDV infection in the Spring-Summer season in A Luoi and Phu Vang was highest (GMT = 1.45), and in Huong Thuy lowest (GMT = 1.31). In addition, in the Fall-Winter season, about 46% of the chickens were infected with NDV and 46.3% with IBDV in Huong Thuy and Phu Vang – two neighbouring districts of Hue City, in which NDV was detected in 54.4% of the chickens in Huong Thuy and 33.9% in Phu Vang. In contrast, IBDV was detected in 41.9% and 52.7% of the chickens respectively in the two districts. The infection was not inter-dependent. Methodically, although the differences in the infection rates were insignificant with the accuracy of 95%, faecal samples showed higher sensitivity in SSIA analyses for both cases of NDV and IBDV infection in comparision with mouth exudates. By SSIA method, results could be read clearly with unaided eyes for a long time after the performance, and it was also proven applicable for cases of haemagglutinating viruses if proper treatments for depletion of animal RBCs’ surface agglutinins could be applied

Sách chỉ dẫn các phông lưu trữ bảo quản tại Trung tâm Lưu trũ Quốc gia IIII

International audienc

Sách chỉ dẫn các phông lưu trữ bảo quản tại Trung tâm Lưu trũ Quốc gia IIII

International audienc

Văn hóa của nhóm nghèo ở Việt Nam - thực trạng và giải pháp = Culture of poverty - current situation and solution

280 tr.; 21 cm

Giáo trình lịch sử kiến trúc thế giới

220 tr. : minh hoạ ; 27 cm