6 research outputs found

    Ultrastructural characteristics of the formation and development of the intercalated disc in the rat left and right working ventricular myocardium on the postnatal ontogenetic stages

    No full text
    Background. Maintenance of the normal distribution of intercellular contacts between cardiomyocytes and preservation of the correct geometry of the intercalated disc are essential conditions for the proper functioning of the heart. Objective. To provide complex ultrastructural evaluation of the mechanism of formation and development of the intercalated disc in rat ventricular myocardium on postnatal stages of development. Methods. Rats at the age of 1, 3, 7, 14, 30 days after birth and mature individuals were used as an object. Transmission electron microscopy, morphometric and statistical methods were applied. In particular, the convolution index was evaluated to characterize quantitative changes of the dynamics of configuration of intercalated disc. Results. It was found that the redistribution of adherens junctions from the lateral surface of cardiomyocytes occurs most rapidly from 3rd to 14th days after birth. The gap junctions trimming occurs due to their internalization with subsequent formation of the annular gap junction profile in the sarcoplasm; these profiles are inserted into the intercalated disc at the last turn. The process of complication of the geometry of intercalated disc is accompanied by the consistent increase of convolution index in the mature myocardium of the left and right ventricles up to 2,46±0,17 and 1,94±0,14, respectively. The predominance of the convolution index in the left ventricle over the levels in the right ventricle was the most prominent on the 14th day of postnatal development (38,1%; p<0,05) and in the mature myocardium – 26,8% (p<0,05). Conclusion. As the result of physiological hypertrophy and adaptive remodeling of cardiomyocyte in the postnatal rat cardiogenesis redistribution of diffusely located intercellular junctions from the periphery to the terminal areas of the cell occurs. The formation of a definitive pattern of distribution of intercellular junctions is completed at the puberty

    The current concept of development of specialized intercellular connections between cardiomyocytes

    No full text
    The understanding of development of interrelation between electric and mechanical contacts in ontogenetic aspect can throw light on the mechanisms underlying heart pathologies related with local disturbance of gap junctions distribution. Despite of significant progress in understanding of structural biology of desmosome, fascia adherents and gap junctions which are a part of an intercalated disk, the detailed characteristic of interrelation between electric and mechanical connections it is not presented still now in the literature. So, data about formation, becoming, development and regulation of specific pattern of intercellular connections remain fragmentary and do not allow to make complete representation about ontogenetic features of formation of communicative contacts between cardiomyocytes. Understanding of development of an intercalated disk young heart, are important for optimization of terms of surgical intervention in pediatrics in occasion of congenital heart diseases. On the other hand, changes of the organization of intercellular contacts, first of all gap junctions, can lead to abnormal conductivity and arrhythmogenesis. Knowledge of similar aspects of development arrhythmias can push to development of new therapeutic methods which will provide higher efficiency of treatment at, for example, ischemic illness of heart or intimate insufficiency

    Proliferation of cardiomyocytes in relation to coronary vessels prenatal development in human heart.

    No full text
    The objectives of our exploration were to define the relationship between the cardiomyocytes proliferative activity and coronary vessels development. We have examined 20 embryos from 4 up to 8 weeks of embryogenesis and 4 human foetus up to 12 weeks of prenatal development. For identification of endothelium we used specific marker CD34; for estimation of proliferative activity of cardiomyocytes - monoclonal antibodies Кі-67 (МІВ-1); besides, we assigned vimentin for identification of smooth myocytes, fibroblasts and perycites. Immunohistochemical reactions carried out with using of the system of visualization LSAB. In the consequence of investigations, the highest cardiomyocyte proliferative activity has been seen at the moment of the absence of cellular component of coronary vessels. Furthermore, the highest amount of endothelia and vimentin-positive cells in the myocardium correlated with the lowest values of cardiomyocyte proliferative index. This fact casts some doubt of the positive coronary vessels effect upon division activity of myocardial cells.Furthermore, decrease in myocardiocyte divisions accompanied by the increase of the coronary components, that confirms previous conclusion. This contradicts to the data of similar processes in mice, what must be taken in account in further investigations

    Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis

    No full text
    Background. There are no complete data on the occurrence of bacteria in the outbreak of chronic inflammation a consequence of their high virulence, or the result of a defect of local mechanisms of immune protection. To answer these questions, as well as to evaluate the nature, severity changes periapical tissues of the tooth and the activity of the inflammatory process in the apex of the tooth root is possible only during the morphological study of biopsy material. Objective. The aim of this work was to study the cellular composition and ultrastructural features of the granulation tissue formed during the current the primary and secondary apical periodontitis. Methods. There were included in the histological examination of the teeth 78, who underwent resection of the apex, followed by a fence of histological material. For ultrastructural studies of granuloma periodontal tissue samples were fixed with 2.5% glutaraldehyde solution, material embedded in Epon-812 («SPI-Pon ™ 812 Epoxy Embedding Kit», USA). The study was conducted using a transmission electron microscopy. Results. In the development of destructive periodontal inflammation are actively involved both specific and non-specific mechanisms of immunity. Conclusion. In primary periodontitis granulation tissue contains a moderate amount of white blood cells of various types, as well as single macrophages and plasma cells with reduced functional activity. In secondary periodontitis granuloma contains numerous mononuclear macrophages, plasma cells and giant multinucleated cells with increased activity, which corresponds to the morphological picture of the formation of immune granulomas. Citation: Gritsenko PI, Petruk NS, Samoylenko AV, Tverdokhleb IV. [Cellular composition and ultrastructure of periapical granulation tissue in primary and secondary chronic periodontitis]. Morphologia. 2014;8(2):14-9. Russian

    Optimization of anesthetic management of surgical treatment of colorectal cancer. Part II: morphological aspects

    No full text
    Background. Interest in the study of the properties of sevoflurane, increased in recent years due to its influence on hemodynamics and morphological condition of the respiratory department of lungs, causes advisability of analyzing the structure of organs directly involved in the reactions of radical surgery for colorectal cancer. Objective. The purpose of the study is comparative analysis of tissue and cell ultrastructure in intraoperative biopsies of the colon in the conditions of application of the standard ataralgesia and combined anesthesia with epidural analgesia. Methods. It was performed a com-parative analysis of morphological data of the colon using total intravenous anesthesia followed analgesia with opiates anal-gesics, and combined techniques using low-flow sevoflurane anesthesia and epidural analgesia, followed by prolonged epidural anesthesia. Results. The central link in the development of pathological changes in the colon wall at anesthesiology maintenance of surgical interventions in the treatment of colorectal cancer is the system of microcirculation. In carrying out radical intervention on the background of the standard total ataralgesia distrophic and destructive changes of endothelial cells organelles cause endothelial dysfunction expressed in microvessels and tissue displays considerable microcirculatory disorders apply to all shell wall of the colon near the affected area of colorectal. Combined low-flow sevoflurane inhalation anesthesia and epidural analgesia with bupivacaine during radical operations followed by containment of endothelial dysfunction by limiting the destructive and degenerative changes in the endothelial cell organelles, stabilization of membranes, preventing spasm of arterioles, stasis and erythrocyte sludge. Conclusion. Methods of epidural analgesia versus opioid analgesia leads to a significant reduction of the phenomena of infiltration, perivascular and interstitial edema in the composition of the intestinal wall in the area of surgical procedure. Citation: Novikov SP, Kirillova LA, Petruk NS, Frolov KB, Tverdokhleb IV. [Optimization of anesthetic management of surgical treatment of colorectal cancer. Part II: morphological aspects]. Morphologia. 2016;10(2):23-30. Russian
    corecore