The effect of cadmium on ascorbic acid, vitamin E and coenzyme Q concentrations in rat kidneys: A possible protective role of coenzyme Q10

Projekat ministarstva br. 03E18 i 03E2

Effects of depkenyl, resekpine and their combination on the antioxidant enzyme activities in the rat brain

Antioxidant enzyme activities: superoxide dismutases (CuZn SOD and Mn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and glutathione reductase (GR) were determined in different brain regions: striatum, hippocampus, hypothalamus with thalamus, as well as in the rest of the brain of rats treated subchronically with L-deprenyl, after a single dose of reserpine and in animals subchronically treated with L-deprenyl and then with reserpine. Our results show that reserpine (p < 0.02), as well as, the combination of deprenyl + reserpine (p<0.01) induced a significant decrease in CAT activity in the rest of the brain and a significant decrease in GSH-Px activity in the hypothalamus with thalamus (p<0.05). Deprenyl expressed no significant effect on antioxidant enzyme activities in the examined brain regions. In experimental group treated with deprenyl and then with reserpine a significant decrease of CuZn SOD activity (p<0.05) in the hippocampus, Mn SOD activity (p<0.05) in the striatum and an increase of CnZn SOD activity (p<0.01) in the rest of the brain were observed. Our results support the opinion that depletion of neuronal catecholamine pool (due to the treatment with reserpine) has a direct influence on antioxidant enzyme activities, while deprenyl probably exerts its effects through interaction with some trophic factors.Aktivnost antioksidativnih enzima: superoksid dismutaze (CuZn SOD i Mn SOD), katalaze (CAT), glutation peroksidaze (GSH-Px), glutation-S-trans-feraze (GST) i glutation reduktaze (GR) u strijatumu, hipokampusu, hipotalamusu sa talamusom, kao i ostatku mozga, praćena je u eksperimentima subhroničnog tretiranja pacova L-deprenilom, akutnog rezerpinom i u kombinaciji oba. Naši rezultati pokazuju da rezerpin (r<0.02), kao i kombinacija deprenila i rezeršša (r<0.01) dovode do smanjenja aktivnosti CAT u ostatku mozga i aktivnosti GSH-Px (r< 0.05) u hipotalamusu sa talamusom. Deprenil ne izaziva značajne promene enzimske aktivnosti u ispitivanim regionima mozga. U eksperimentalnoj grupi tretiranoj deprenilom, pa rezerpinom dolazi do značajnog smanjenja aktivnosti CuZn SOD (p< 0.005) hipokampusa, aktivnosti Mn SOD strijatuma (p<0i)5) i povećnja aktivnosti CuZn SOD (r<0.01) ostatka mozga. Ovi rezultati ukazuju da iscriljenje pula kateholamina (usled tretmana rezerpinom) ima direktan uticaj na aktivnost antioksidativnih enzima, dok su za efekte deprenila značajne interakcije sa nekim trofičkim faktorima.nul

The effect of cadmium on ascorbic acid, vitamin E and coenzyme Q concentrations in rat kidneys: A possible protective role of coenzyme Q10

Projekat ministarstva br. 03E18 i 03E2

Optimization of carboxymethylcellulose production

The aim of the study was to obtain water soluble carboxymethylcellulose (CMC) with a high degree of polymerization (DP gt 600) and high degree of substitution (DS gt 800), in industrial conditions, from easily degradable eucalyptus cellulose. The influence of temperature and of the NaOH:MCAA molar ratio cellulose on the structural and degradative changes in cellulose macromolecules during the process was investigated using ethanol as the reaction medium. The structural transformations during alkalization and etherification were investigated by X-ray diffraction. The degradative changes in the macromolecule were followed by determining the degree of polymerization. The degree of substitution and other chemical characteristics were determined by standard analytical methods. The thermal behaviour of the samples was studied by using a Perkin Elmer DSC-2 instrument. The rheological properties of the CMC solutions were also studied

The electrochemical behavior of commercial clarithromycin and spectroscopic detection of its structural changes

The aim of the present study was to examine the behavior of commercial clarithromycin, Clathrocyn (R), comparing to the results previously obtained using pure clarithromycin under the same experimental conditions. The study was performed by cyclic linear sweep voltammetry and the bulk of electrolyte was analyzed by FTIR spectroscopy and HPLC. At gold electrode, in the range of -1.2 V to 1.0 V vs. SCE in 0.05 M NaHCO3, the electrochemical determination of pure clarithromycin was previously defined by four anodic and one cathodic reproductive peaks, and commercial clarithromycin is defined by reproductive one anodic peak which appears from 0.60 to 0.80 V vs. SCE. Previously, FTIR analysis of the bulk electrolyte showed the apparent changes in pure clarithromycin molecule structure: in the ester bond of the lactone and in ethers and acetal bonds. FTIR analysis of the bulk electrolyte also showed changes in the molecule structure in commercial-tablets form of clarithromycin: indicating disappearance of lactone structure and changes of carbonyl group at position 9