The use of framework agreements in public procurement

Zadávání veřejných zakázek na základě rámcových smluv Rámcové smlouvy v oblasti veřejných zakázek představují zvláštní druh veřejné zakázky usnadňující postup při pořizování opakujících se obdobných dodávek, služeb či stavebních prací. Hlavním cílem této diplomové práce je provedení komplexního rozboru právní úpravy institutu rámcových smluv v oblasti práva veřejných zakázek. Diplomová práce vychází z úpravy platného zákona č. 137/2006 Sb., o veřejných zakázkách a je rozdělena do 5 kapitol. První kapitola se věnuje úpravě rámcových smluv v českém právním řádu a v právu Evropské unie a také vývoji této právní úpravy. Druhá kapitola se zaměřuje na základní zásady a pojmy zákona č. 137/2006 Sb., o veřejných zakázkách, které jsou s ohledem na téma diplomové práce relevantní. První část této kapitoly vymezuje základní zásady obsažené v zákoně i v předpisech Evropské unie. Druhá část této kapitoly pak podává základní informace o nejdůležitějších institutech zákona. Třetí kapitola se zabývá první fází při zadávání veřejných zakázek s použitím rámcové smlouvy, tedy uzavřením rámcové smlouvy a v rámci toho též průběhem zadávacího řízení. Čtvrtá kapitola navazuje na třetí a je věnována druhé fázi tohoto zadávacího řízení a to zdávání veřejných zakázek na základě uzavřené rámcové smlouvy. Pátá kapitola zkoumá...The use of framework agreements in public procurement Framework agreements in public procurement are a special type of public contracts facilitating the process of the award of supplies, services or works. The main purpose of this master thesis is to undertake a comprehensive analysis of the provisions of framework agreements in the field of public procurement. The master thesis is based on the current provision of the Act No. 137/2006 Coll., on Public Procurements and is divided into five chapters. Chapter one deals with the adaptation of framework agreements in the legislation both in the Czech Republic and in the European Union and as well with the development of this legislation. Chapter two focuses on the basic principles and concepts of the Act No. 137/2006 Coll., on Public Procurements which are relevant for the topic of this master thesis. The first part of this chapter defines the basic principles contained in the Act and the in the legislation of the European Union. The second part of this chapter then presents basic information about the most important institutes of the Act. The third chapter deals with the first phase of the awarding of public contracts with the use of the framework agreement, thus concluding the framework agreement and also in the conduct of the procurement procedure....Department of Business LawKatedra obchodního právaFaculty of LawPrávnická fakult

Yeast diversity in new, still fermenting wine "federweisser"

The aim of this study was to isolate and identify yeasts in different new wine "federweisser" samples. We collected the samples at the end of the August 2015 and in the middle of the September 2015. Used 15 new wine samples in this study (5 white and 10 red) were from the local Slovak winemakers. Irsai Oliver (3), Moravian Muscat (2), Agria/Turan (1), Dornfelder (3), Blue Frankish (3), Pinot Noir (1) and Saint Laurent (2). Three cultivation media were used for detection of yeasts in "federweisser" samples. Malt extract agar base (MEA), Wort agar (WA) and Wild yeast medium (WYM) were used for the cultivation of yeasts. Cultivation was performed by spread plate method. Ethanol/formic acid extraction procedure was used for preparation of samples. MALDI-TOF Mass Spectrometer (Microflex LT/SH) (Bruker Daltonics, Germany) was used for the identification of yeasts. We identified seven different strains of Saccharomyces cerevisiae (23; 70%), two strains of Kloeckera apiculata [teleomorph Hanseniaspora uvarum] (7; 21%), and one strain of Pichia kluyveri (1; 3%), Pichia occidentalis [anamorph Candida sorbosa] (1; 3%) and Metschnikowia pulcherrima (1; 3%) in 15 new wine "federweisser" samples. Saccharomyces cerevisiae was dominant species in each new wine sample, and formed creamy convex colonies with circular edge. Metschnikowia pulcherrima formed convex to pulvinate, circular white-pink colored colonies, Kloeckera apiculata formed flat, circular smooth colonies with turquoise center with gray edge, Pichia occidentalis formed irregular pulvinate light-cream colored colonies, and Pichia kluyveri formed turquoise, convex, undulate and smooth colonies on Malt extract agar base with bromocresol green

Bee bread - perspective source of bioactive compounds for future

Bee bread is product with long history used mainly in folk medicine. Nowadays, bee bread is growing in commercial interest due to its high nutritional properties. The objective of this study was to determine biological activity of ethanolic extract of bee bread obtained from selected region of Ukraine - Poltava oblast, Kirovohrad oblast, Vinnica oblast, Kyiv oblast, Dnepropetrovsk oblast. The antioxidant activity was measured with the radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical as well as phosphomolybdenum assay. Total polyphenol content was determined with Folin-Ciocalteau reagent and total flavonoid content by aluminium-chloride method. Secondary was also evaluated antimicrobial activity in bee bread samples with disc diffusion method and minimum inhibitory concentrations. Antioxidant activity expressed as mg TEAC per g of dry weight (Trolox equivalent antioxidant capacity) was the highest in bee bread from Poltava oblast in DPPH and also phosphomolybdenum method. Samples of bee bread contained high levels of total polyphenols (12.36 - 18.24 mg GAE - gallic acid equivalent per g of dry weight) and flavonoids (13.56 - 18.24 μg QE - quercetin equivalent per g of dry weight) with the best values of bee bread from Poltava oblast. An elevated level of antioxidant potential in the bee bread determines its biological properties, which conditioned of the biological active substances. The best antibacterial activity of bee bred with disc diffusion method was found against Bacillus thuringiensis CCM 19. The antibacterial activity inhibited by the bee bread extract in the present study indicate that best minimal inhibition concentration was against bacteria Escherichia coli CCM 3988 and Salmonella enterica subs. enterica CCM 3807

Application of lavender and rosemary essential oils improvement of the microbiological quality of chicken quarters

The aim of the present work was monitoring of chicken quarters microbiological indicators after treatment by ethylenediaminetetraacetate (EDTA), lavender (Lavandula angustifolia L.) and rosemary (Rosmarinus officinalis L.) essential oil, stored under vacuum packaging, at 4 ±0.5°C for a period of 16 days. The following treatments of chicken quarters were used: Air-packaging control samples, control vacuum-packaging samples, vacuum-packaging with EDTA solution 1.50% w/w, control samples, vacuum-packaging with Lavandula angustifolia essential oil at concentrations 0.2% v/w and vacuum-packaging with Rosmarinus officinalis essential oil at concentration 0.2% v/w. The quality assessment of all samples was established by microbiological analysis. Sampling was carried out after certain time intervals: 0, 4, 8, 12 and 16 days. Chicken quarters were stored under vacuum packaging, at 4 ±0.5°C during experiment. Microbiological analyses were conducted by using standard microbiological methods. Anaerobic plate count were determined using Plate Count Agar, after incubation for 2 days at 35°C under anaerobic condition. Pseudomonas spp. were determined on Pseudomonas Isolation agar after incubation at 48 h at 25°C. For lactic acid bacteria were inoculated into Rogosa and Sharpe agar after incubation 48-78 h at 37°C in an aerobic atmosphere supplemented with carbon dioxide (5% CO2). For members of the family Enterobacteriaceae violet red bile glucose agar were used and samples were incubated at 37°C for 24 h. The initial APC value of chicken quarter was 3.00 log CFU.g-1 on 0 day. The number of anaerobic plate count ranged from 3.00 log CFU.g-1 in all tested group on 0 day to 6.11 log CFU.g-1 on 16 day in control group stored in air condition. The initial LAC value of chicken quarter was 3.00 log CFU.g-1 on 0 day. The number of lactic acid bacteria ranged from 3.00 log CFU.g-1 in all tested group on 0 day to 3.58 log CFU.g-1 on 16 day in control group stored in air condition. The initial Enterobacteriacea genera value of chicken quarter was 2.00 log CFU.g-1 on 0 day. Presences of these bacteria were found on all groups at 16 days. The results of this present study suggest the possibility of application the Lavandula angustifolia and Rosmarinus officinalis essential oil as natural food preservatives and potential sources of antimicrobial ingredients for food industry.

The extension of shelf-life of chicken meat after application of caraway and anise essential oils and vacuum packaging

The effect of caraway (CEO) and anise (AEO) essential oils as well as vacuum packaging (VP) in extending of the shelf life of fresh chicken breast meat stored at 4 °C was investigated. CEO and AEO were used at concentrations 0.2% v/w with and without VP. Microbiological properties of chicken breast meat were monitored over a 16 day period. The microbiological parameters as the anaerobic plate count (AC), Enterobacteraceae, lactic acid bacteria and Pseudomonas spp. counts were detected. The anaerobic plate counts ranged from 2.77 log cfu.g-1 in all tested group on 0 day to 5.45 log cfu.g-1 on 16 day in control group stored in air condition. The number of lactic acid bacteria ranged from 3.20 log cfu.g-1 in all tested group on 0 day to 4.75 log cfu.g-1 on 16 day in control group stored in air condition. Enterobacteriaceae counts ranged from 0.00 to 4.25 log cfu.g-1on 16 day in control group stored in air condition. The number of Pseudomonas spp. ranged from 0.00 log cfu.g-1 in all tested group on 0 day to 2.65 log cfu.g-1 on 16 day in control group stored in air condition. Statistically significant differences (P≤0.001) were found among tested group in all tested microorganisms. Among the antimicrobial combination treatments were examined in the study, the as application of vacuum packaging, EDTA, and essential oils were the most effective against the growth of lactic acid bacteria and Enterobactericeae and to a less extent on anaerobic plate count. The results of this present study suggest the possibility of using the essential oil of caraway and anise as natural food preservatives and potential source of antimicrobial ingredients for chicken breast meat

Bioactive fractions from cantabrian anchovy (Engraulis encrarischolus) viscera

The potential of cantabrian anchovy (Engraulis encrarischolus) viscera as a source of bioactive compounds is of interest for both, pharmaceutical and food industries. Cantabrian anchovy guts and heads were freeze-dried, extracted with methanol and subjected to fractionation by solvent partitioning using hexane, ethyl acetate, and butanol. Fractions were tested for antimutagenic, antioxidant, antifungal, and antibacterial activity using the Ames test; DPPH, ABTS, and FRAP assays; the radial grown inhibition assay; and the microbroth dilution method, respectively. Five fractions were obtained from the anchovy gut methanolic extract, in addition to the hexane- (HF), ethyl acetate- (EAF), and butanol-soluble (BF) fractions, an aqueous-soluble fraction (ALF) and precipitated crystals (ACF) in this were also obtained. HF and EAF resulted to be antimutagenic, HF and ALF showed antifungal activity, BF and ACF showed the highest antioxidant potential, and HF and BF were antibacterial against several strains. Anchovy gut, which to the present study had not been reported for any bioactivity, has antimutagenic, antifungal, antioxidant, and antibacterial compounds, which need to be isolated for full characterization and study.Authors wish to acknowledge CONACyT for the financial support granted to Burgos-Hernández, A., (234469) and Rosas‑Burgos, E.C., (234051) to carry out a sabbatical-research staying at the Universidad Miguel Hernández de Elche at Orihuela, Alicante, Spain

Cesta ke zdraví

Title in English: Journey to Health: Suggestions for the implementation of oncological prevention in teaching health education The methodological material “Journey to Health. Suggestions for the implementation of oncological prevention in teaching health education” is focused on the prevention of oncological diseases and it is intended for teachers of upper primary schools for teaching Health Education, eventually Biology. Cancer is nowadays one of the most common diseases. Prevention plays important role in decreasing the incidence of oncological diseases, therefore it is very important that the basic knowledge about prevention of oncological diseases be given to primary school pupils. The main purpose is to acquaint pupils with primary and secondary prevention of oncological diseases and to increase their health literacy in this area. The material includes a suggestion for 20 lessons. Each of them contains professional information for teachers’ theoretical preparation, suggestions for the lesson, a worksheet with solutions, and proposal activities on the topic

Obligation to Pay and the Consequences of Its Violation in terms of Sales Agreement

Import 04/07/2011Kupní smlouva patří k nejvýznamnějším smluvním typům. V moderní společnosti, založené na finančních tocích neexistuje člověk, který by se s ní nesetkal – ať již při prodeji v obchodě, nebo v rámci své podnikatelské činnosti. Předmětem bakalářské práce je kupní smlouva realizovaná ve vztazích mezi podnikateli. Cílem práce je analyzovat povinnosti platit. Druhotným cílem je navrhnout v některých případech i znění smluvních podmínek odchylně od úpravy v zákoně. Tento cíl je naplňován v rámci tzv. povinnosti platit a formulaci platební podmínky. Samozřejmě tam, kde to dispozitivní povaha ustanovení dovoluje.The purchase agreement is one of the most important contract types. In modern society, based on financial flows there is a man who would not meet with it - whether by sale in a shop or in the course of its business. Subject of this thesis is the purchase of the agreement in relations between professionals. The aim is to analyze the obligation to pay. A secondary objective is to design in some cases the wording of contractual terms, notwithstanding changes in the Law. This objective is pursued within the so-called obligation to pay, and the formulation of terms of payment. Of course, where the dispositive nature of provision allows.119 - Katedra právavelmi dobř