Energy Dispersive X-Ray Microanalysis of the Dentin in Rat Molars after Corticosteroid Treatment

The aim of this study was to investigate whether the calcium (Ca) and phosphorus (P) composition of corticosteroid induced dentin was the same as in normally developed dentin. Seven rats were given corticosteroids intravenously and three rats served as controls. Energy dispersive X-ray microanalysis (EDX) was carried out on the axially sawn roots of the molars. Measurements were made at 20 sites, equally distributed in the buccal, mesial, lingual and distal direction. The results showed that the Ca/P ratio (weight %) was slightly above 2.0 in both the experimental and the control group, indicating that the corticosteroid induced dentin had a normal Ca/P ratio. However, different degrees of mineralization were found in different directions of the roots

ApoLänk decreases patient medication discrepancies at discharge: initial experience from a Swedish bedside pharmacy service

Objective: To develop a working model so that dispensing pharmacists, patients and medical staff can work together to identify, solve and prevent problems associated with discharge prescriptions. Method: A routine was developed for communication between pharmacists, patients and medical staff and for dispensing drugs at the bedside. Hospital medication lists and prescriptions from the pharmacy and healthcare databases were simultaneously assessed. The study was carried out in two neurological wards in a Swedish university hospital. Results: Major shortcomings were identified in the coordination of pharmacy and healthcare records and in patients’ ability to take responsibility for their medication at discharge. Discussion between patients and staff allowed discrepancies in communication and documentation to be corrected. Cooperation between pharmacists and nurses was perceived as being very positive and important. Conclusions: The Apolänk service was appreciated by nurses, is almost cost neutral and reduces discrepancies that have the potential to cause patient harm

Experiences of physical activity during pregnancy in Danish nulliparous women with a physically active life before pregnancy. A qualitative study

<p>Abstract</p> <p>Background</p> <p>National guidelines recommend that healthy pregnant women take 30 minutes or more of moderate exercise a day. Most women reduce the level of physical activity during pregnancy but only a few studies of women's experiences of physical activity during pregnancy exist. The aim of the present study was to elucidate experiences and views of leisure time physical activity during pregnancy in nulliparous women who were physically active prior to their pregnancy.</p> <p>Methods</p> <p>A qualitative study was conducted by means of personal interviews. Nineteen women, all with a moderate pre-pregnancy level of physical activity but with different levels of physical activity during pregnancy, participated in the study. Content analysis was applied.</p> <p>Results</p> <p>In the analyses of experiences and views of physical activities during pregnancy, four categories and nine sub-categories were developed: <it>Physical activity as a lifestyle </it>(Habit and Desire to continue), <it>Body awareness </it>(Pregnancy-related discomfort, Having a complicated pregnancy and A growing body), <it>Carefulness </it>(Feelings of worry and Balancing worry and sense of security) and <it>Sense of benefit </it>(Feelings of happiness and Physical well-being).</p> <p>Conclusion</p> <p>As other studies have also shown, women find that the discomfort and complications associated with pregnancy, the growing body, and a sense of insecurity with physical activity are barriers to maintaining former levels of physical activity. This study adds a new perspective by describing women's perceptions of these barriers and of overcoming them - thus, when pregnant, the majority of the women do not cease to be physically active but continue to be so. Barriers are overcome by applying one's own experience, looking to role models, mirroring the activities of other pregnant women and following the advice of experts (midwives/physiotherapists). Women then continue to be physically active during pregnancy, most often to a lesser extent or in alternative activities, and derive considerable enjoyment and physical well-being from this.</p

Salicylketoximes as inhibitors of Glucose Transporters

Some derivatives of the 4-arylsalicylketoximes series displayed inhibitory effects on glucose transport and on cell proliferation in several biological assays,[1] resulting to be effective GLUT1 inhibitors also in GLUT1-containing giant vesicles. GLUT1 is one of the 14 glucose transporter isoforms, widely overexpressed in many cancer types. Thus, for the discovered properties, the oximes of interest represent interesting candidates for anticancer therapy. Variously substituted 4-arylsalicylketoximes (3, Fig.1) were synthetized via Suzuki cross-coupling and a subsequent condensation of the resulting biaryl-ketone intermediates with hydroxylamine hydrochloride. [1] Rat GLUT1 membrane proteins were produced by Pichia Pastoris cultures, and purified following GLUT1 purification protocols, [2] which were largely revised to avoid the protein cleavage. Compounds 3a, 3b, 3e, and 3f efficiently inhibited glucose uptake in GLUT1-containing giant vesicle assays. [3] To study the nature of the binding process between GLUT1 and the synthetic compounds, many crystallization attempts were set up with 3a and 3e using Lipidic Cubic Phase method, which produced many small crystals. Since many isoforms of GLUTs are overexpressed in cancer cells, inhibition of other GLUT isoforms, such as GLUT3, will be tested in the near future. In conclusion, 4-arylsalicylketoximes showed good inhibition of GLUT1 isoform. First results from GLUT3-giant vesicles assays revealed that, within this series of compounds, 3a is the most selective GLUT1-inhibitor. Further assays with GLUTs-containing giant vesicle and crystallization attempts are currently underway. [1] Granchi C, Qian Y, Lee H.Y, Paterni I, Pasero C, Iegre J, Carlson K. E, Tuccinardi T, Chen X, Katzenellenbogen J. A, Hergenrother P. J, Minutolo F, ChemMedChem. 2015; 1892–1900. [2] Venskutonyté R, Elbing K, Lindkvist-Petersson K, Methods Mol Biol. 2018; 1713, 1–13. [3] Hansen J.H, Elbing K, Thompson J.R, Malmstadt N, Lindkvist-Petersson K, Chem. Commun. 2015; 51, 2316–2319

Oat Polar Lipids Improve Cardiometabolic-Related Markers after Breakfast and a Subsequent Standardized Lunch : A Randomized Crossover Study in Healthy Young Adults

It has been suggested that intake of polar lipids may beneficially modulate various metabolic variables. The purpose of this study was to evaluate the effect of oat polar lipids on postprandial and second meal glycemic regulation, blood lipids, gastrointestinal hormones, and subjective appetite-related variables in healthy humans. In a randomized design, twenty healthy subjects ingested four liquid cereal-based test beverages (42 g of available carbohydrates) containing: i. 30 g of oat oil with a low concentration (4%) of polar lipids (PLL), ii. 30 g of oat oil containing a high concentration (40%) of polar lipids (PLH), iii. 30 g of rapeseed oil (RSO), and iv. no added lipids (NL). The products were served as breakfast meals followed by a standardized lunch. Test variables were measured at fasting and during 3 h after breakfast and two additional hours following a standardized lunch. PLH reduced glucose and insulin responses after breakfast (0-120 min) compared to RSO, and after lunch (210-330 min) compared to RSO and PLL (p < 0.05). Compared to RSO, PLH resulted in increased concentrations of the gut hormones GLP-1 and PYY after the standardized lunch (p < 0.05). The results suggest that oat polar lipids have potential nutraceutical properties by modulating acute and second meal postprandial metabolic responses

Cambios en los niveles sanguíneos de vitaminas A y E, selenio, leucocitos y expresión de moléculas de BR adhesión para migración en neutrófilos en vacas lecheras alrededor del parto

Blood samples from ten Swedish dairy cows were taken one month before calving, at calving, and one month after calving. The blood concentration of vitamins A and E, and the trace element selenium (Se) were analysed, as well as the total and differential white blood cell counts,a nd the presenceo f CD62L and CD18 adhesionm oleculeso n blood neutrophils. The levels of vitamins A and E decreased significantly at calving, while whole blood Se increased Leukocytosisw as detectedat calving, explainedm ainly by neutrophilia.T he number of lymphocytest endedt o decreasea t calvrng.T he proportion of CD62L- neutrophilsd ecreased sisnlficantlv at this ttmeLa susceptibilidada las enfermedadesin fecciosas,tales como la mastitis, en vacas lecheras es muy alta durante el período alrededor del parto. Los mecanismos de defensa del animal están disminuidos desde aproximadamente tres semanas antes del parto hasta tres semanas despues. Las causas no son claras, sin embargo ocurren una serie de cambios metabòlicos y hormonales durante este periodo, los cuales pueden influr negativamente sobre el sistema inmunitario. En este ensayo se tomaron muestras de sangre de diez vacas lecheras (raza Roja y Blanca Sueca) un mes antes del parto, al parto y un mes después del mismo. Las concentracioness éricasd e vitaminas A y E, y los niveles de selenio (Se) en sangre fueron analizados, como así también el número y diferenciación ieucocitarios, y la presencia de moléculas de adhesión para migración CD62L y CD18 en neutrófilos. Los niveles de vitaminas A y E disminuyeron significativamente al parto, mientras que los de Se aumentaron. En este momento también fue detectada una leucocitosis, expiicada por neutrofilia y linfopenia. Las proporciones de neutrófilos positivos a CD62L disminuyeron significativamente al parto. Los cambioso bservadose n 1osn iveles sanguíneos de vitaminas y Se fueron principalmente en respuesta a la formación de calostro, a cambios en la ingesta de materia seca y a el metabolismo ruminal. Los bajos niveles de vitaminas A y E pueden tener efectos negativos en el funclonamiento del sistema inmunitario. Las menores proporciones de neutrófilos positivos a CD62L al parto resultarían en menor migración hacla los tejidos, contribuyendoa un aumento en la susceptibilidada enfetmedades como mastiti

Glucose transporters: production, crystallization and inhibition

Glucose transporters (GLUTs) comprise a family of 14 membrane proteins that regulate glucose uptake into the cell. Different types of GLUTs are expressed in various tissues and play a crucial role in glucose metabolism. Cancer cells are highly dependant on glucose and therefore GLUTs are possible drug targets for cancer therapy. In order to block the glucose uptake facilitated by GLUTs, various inhibitors are studied and both natural and synthetic compounds having an inhibitory effect on glucose uptake have been discovered. High resolution X-ray structure of the GLUT-inhibitor complex would provide a detailed understanding of protein-inhibitor interactions and contribute to facilitating the development of new derivatives. The focus of this study is on a glucose transporter 1 (GLUT1). The GLUT1 has been produced and crystallization trials set up, which resulted in microcrystals. A series of salicylketoxime based compounds have been shown to inhibit GLUT1 and two lead compounds displaying the highest inhibition have been identified in a giant vesicle assay. The main goal of the study is to determine the structure of the GLUT1 with selected inhibitors. Moreover, studies on one more glucose transporter GLUT3 are carried out to investigate the selectivity of the salcylketoxime compounds

Comparable survival in rats with intracranial glioblastoma irradiated with single-fraction conventional radiotherapy or FLASH radiotherapy

BackgroundRadiotherapy increases survival in patients with glioblastoma. However, the prescribed dose is limited by unwanted side effects on normal tissue. Previous experimental studies have shown that FLASH radiotherapy (FLASH-RT) can reduce these side effects. Still, it is important to establish an equal anti-tumor efficacy comparing FLASH-RT to conventional radiotherapy (CONV-RT).MethodsFully immunocompetent Fischer 344 rats with the GFP-positive NS1 intracranial glioblastoma model were irradiated with CONV-RT or FLASH-RT in one fraction of 20 Gy, 25 Gy or 30 Gy. Animals were monitored for survival and acute dermal side effects. The brains were harvested upon euthanasia and tumors were examined post mortem.ResultsSurvival was significantly increased in animals irradiated with CONV-RT and FLASH-RT at 20 Gy and 25 Gy compared to control animals. The longest survival was reached in animals irradiated with FLASH-RT and CONV-RT at 25 Gy. Irradiation at 30 Gy did not lead to increased survival, despite smaller tumors. Tumor size correlated inversely with irradiation dose, both in animals treated with CONV-RT and FLASH-RT. Acute dermal side effects were mild, but only a small proportion of the animals were alive for evaluation of those side effects.ConclusionThe dose response was similar for CONV-RT and FLASH-RT in the present model. Tumor size upon the time of euthanasia correlated inversely with the irradiation dose