Zinc bioaccumulation by microbial consortium isolated from nickel smelter sludge disposal site

Heavy metal pollution is one of the most important environmental issues of today. Bioremediation by microorganisms is one of technologies extensively used for pollution treatment. In this study, we investigated the heavy metal resistance and zinc bioaccumulation by microbial consortium isolated from nickel sludge disposal site near Sereď (Slovakia). The composition of consortium was analyzed based on MALDI-TOF MS of cultivable bacteria and we have shown that the consortium was dominated by bacteria of genus Arthrobacter. While consortium showed very good growth in the zinc presence, it was able to remove only 15 % of zinc from liquid media. Selected members of consortia have shown lower growth rates in the zinc presence but selected isolates have shown much higher bioaccumulation abilities compared to whole consortium (up to 90 % of zinc removal for NH1 strain). Bioremediation is frequently accelerated through injection of native microbiota into a contaminated area. Based on data obtained in this study, we can conclude that careful selection of native microbiota could lead to the identification of bacteria with increased bioaccumulation abilities

Biosorption and Bioaccumulation Abilities of Actinomycetes/Streptomycetes Isolated from Metal Contaminated Sites

Heavy metal pollution is of great concern. Due to expansion of industrial activities, a large amount of metal is released into the environment, disturbing its fragile balance. Conventional methods of remediation of heavy metal-polluted soil and water are expensive and inefficient. Therefore, new techniques are needed to provide environmentally friendly and highly selective remediation. Streptomycetes, with their unique growth characteristics, ability to form spores and mycelia, and relatively rapid colonization of substrates, act as suitable agents for bioremediation of metals and organic compounds in polluted soil and water. A variety of mechanisms could be involved in reduction of metals in the environment, e.g., sorption to exopolymers, precipitation, biosorption and bioaccumulation. Studies performed on biosorption and bioaccumulation potential of streptomycetes could be used as a basis for further development in this field. Streptomycetes are of interest because of their ability to survive in environments contaminated by metals through the production of a wide range of metal ion chelators, such as siderophores, which provide protection from the negative effects of heavy metals or specific uptake for specialized metabolic processes. Many strains also have the equally important characteristic of resistance to high concentrations of heavy metals

Actinomyces glycerinitolerans strain G10T was isolated from rumen fluid, can metabolize a range of various substrates including complex carbohydrates to organic acids. Here, we report a -Mbp draft genome of Actinomyces glycerinitolerans.

Actinomyces glycerinitolerans strain G10T was isolated from rumen fluid, can metabolize a range of various substrates including complex carbohydrates to organic acids. Here, we report a -Mbp draft genome of Actinomyces glycerinitolerans

Draft genome sequence of Actinomyces glycerinitolerans strain G10T, isolated from sheep rumen fluid

Actinomyces glycerinitolerans strain G10T, which was isolated from sheep rumen fluid, can metabolize a range of substrates, including complex carbohydrates to organic acids (OAs). Here, we report a 3.69-Mbp draft genome of Actinomyces glycerinitolerans

Actinomyces glycerinitolerans strain G10T was isolated from rumen fluid, can metabolize a range of various substrates including complex carbohydrates to organic acids. Here, we report a -Mbp draft genome of Actinomyces glycerinitolerans.

Actinomyces glycerinitolerans strain G10T was isolated from rumen fluid, can metabolize a range of various substrates including complex carbohydrates to organic acids. Here, we report a -Mbp draft genome of Actinomyces glycerinitolerans

Morphological variability of Fomes fomentarius basidiomata based on literature data

Currently two morphological species of the genus Fomes (Polyporaceae, Basidiomycota) are known: F. fomentarius (L.) Fr. and F. fasciatus (Sw.) Cooke. Both species are very important in the decomposition of wood and in the nutrient cycling in forest ecosystems. Moreover, F. fomentariusis also known as a source of medicinal and nutraceutical products. Recently the existence of three separate ITS lineages/sublineages among F. fomentarius strains has been clearly established - A1 (the strains isolated from North America), A2 (only from Europe) and B (from Europe and Asia). In this review the current knowledge of the morphological variability of F. fomentarius basidiomata has been summarized in respect of the reliable separation of its lineages/sublineages. Micro- and macrofeatures and geographic variability of the basidiomata have been described. Morphological traits of the F. fomentarius basidiomata can vary due to geographical distribution. These phenotypic differences can be based on the presence of several groups or interactions between the genotype and environment. However, no clear differences between basidiomata, useful for reliable separation of the lineages/sublineages, have been observed so far. A recent description in North American Fomes research articles is based on basidiomata of the A1 sublineage. It is most possible that the descriptions in European literature are based on basidiomata of two other lineages/sublineages and thus we cannot discriminate between them. In the future a detailed study of the macro- and microtraits - pileus and pore surface colors, basidioma size, depth of tube layer, and basidiospore size - is therefore proposed for reliable separation of the lineages/sublineages of F. fomentarius

Genetic diversity of Acinetobacter spp. adapted to heavy metal polluted environments

Multiple metallotolerant bacterial strains were isolated from soil and drainage water samples collected from three industrially heavy metals polluted areas in Slovakia. Obtained bacterial isolates were identified using MALDI-TOF mass spectrometry and bacterial isolates that belonged to the Acinetobacter genus were subjected for the further study. A. calcoaceticus was found to be prevalent species among analyzed Acinetobacter spp. strains, followed by A. lwoffii and A. johnsonii. A. calcoaceticus strains exhibited higher minimum inhibitory concentration to Mn, Zn, and Cu cations compared to A. lwoffii and A. johnsonii. On the other hand minimum inhibitory concentration to Co and Ni were identical in all Acinetobacter spp. isolates. Genetic analyses demonstrated multiple plasmids presence in A. lwoffii and A. johnsonii but not in A. calcoaceticus. Using ERIC-PCR the presence of two different genotypes of A. calcoaceticus was detected in heavy metal polluted environments in Slovakia

Actinomyces glycerinitolerans strain G10T was isolated from rumen fluid, can metabolize a range of various substrates including complex carbohydrates to organic acids. Here, we report a -Mbp draft genome of Actinomyces glycerinitolerans.

Actinomyces glycerinitolerans strain G10T was isolated from rumen fluid, can metabolize a range of various substrates including complex carbohydrates to organic acids. Here, we report a -Mbp draft genome of Actinomyces glycerinitolerans

Molecular Data Reveal Unrecognized Diversity in the European Ganoderma resinaceum

Ganoderma resinaceum Boud. is commonly found in Mediterranean region, but rarely in Western, Central or Eastern Europe. It is a parasitic basidiomycetous fungus causing stem decay—especially in urban trees. A collection of nine fungal specimens from Slovakia (Central Europe), morphologically identified as G. resinaceum, was recently studied on the basis of sequence data from the internal transcribed spacer (ITS) regions. Analyses showed that the collections clustered into two separate groups. In this study—for the first time—the sequences of other molecular markers, namely partial translation elongation factor (tef1-α) region and partial 25S large subunit ribosomal RNA gene (25S LSU rRNA), as well as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI–TOF MS) were obtained and used to evaluate the genetic variability of G. resinaceum. All these analyses confirm the existence of two previously unrecognized genotypes within the morphospecies

Candidatus Cryptoplasma Associated with Green Lizards and Ixodes ricinus Ticks, Slovakia, 2004–2011

During 2004–2011, we collected green lizards and Ixodes ricinus ticks in Slovak Karst National Park in Slovakia; 90% (36/40) of lizards and 37% of ticks removed from lizards were infected with family Anaplasmataceae bacteria. Only Candidatus Cryptoplasma sp. REP (reptile) was identified in these samples. Green lizards transmit this bacterium