Destriping Cosmic Microwave Background Polarimeter data

Destriping is a well-established technique for removing low-frequency correlated noise from Cosmic Microwave Background (CMB) survey data. In this paper we present a destriping algorithm tailored to data from a polarimeter, i.e. an instrument where each channel independently measures the polarization of the input signal. We also describe a fully parallel implementation in Python released as Free Software and analyze its results and performance on simulated datasets, both the design case of signal and correlated noise, and with additional systematic effects. Finally we apply the algorithm to 30 days of 37.5 GHz polarized microwave data gathered from the B-Machine experiment, developed at UCSB. The B-Machine data and destriped maps are made publicly available. The purpose is the development of a scalable software tool to be applied to the upcoming 12 months of temperature and polarization data from LATTE (Low frequency All sky TemperaTure Experiment) at 8 GHz and to even larger datasets.Comment: Submitted to Astronomy and Computing on 15th August 2013, published 7th November 201

Regio- and Enantioselective Alkane Hydroxylation with Engineered Cytochromes P450 BM-3

Cytochrome P450 ΒΜ-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. BM-3 variant 9-10A-A328V hydroxylates octane at the 2-position to form S-2-octanol (40% ee). Another variant, 1-12G, also hydroxylates alkanes larger than hexane primarily at the 2-position but forms R-2-alcohols (40−55% ee). These biocatalysts are highly active (rates up to 400 min-1) and support thousands of product turnovers. The regio- and enantioselectivities are retained in whole-cell biotransformations with Escherichia coli, where the engineered P450s can be expressed at high levels and the cofactor is supplied endogenously

Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol

We have determined the X-ray crystal structures of the NADH-dependent alcohol dehydrogenase LlAdhA from Lactococcus lactis and its laboratory-evolved variant LlAdhA^(RE1) at 1.9 Å and 2.5 Å resolution, respectively. LlAdhA^(RE1), which contains three amino acid mutations (Y50F, I212T, and L264V), was engineered to increase the microbial production of isobutanol (2-methylpropan-1-ol) from isobutyraldehyde (2-methylpropanal). Structural comparison of LlAdhA and LlAdhA^(RE1) indicates that the enhanced activity on isobutyraldehyde stems from increases in the protein's active site size, hydrophobicity, and substrate access. Further structure-guided mutagenesis generated a quadruple mutant (Y50F/N110S/I212T/L264V), whose K_M for isobutyraldehyde is ∼17-fold lower and catalytic efficiency (k_(cat)/K_M) is ∼160-fold higher than wild-type LlAdhA. Combining detailed structural information and directed evolution, we have achieved significant improvements in non-native alcohol dehydrogenase activity that will facilitate the production of next-generation fuels such as isobutanol from renewable resources

Stand-off Molecular Composition Analysis

Composition of distant stars can be explored by observing absorption spectra. Stars produce nearly blackbody radiation that passes through the cloud of vaporized material surrounding the star. Characteristic absorption lines are discernible with a spectrometer, and atomic composition is investigated by comparing spectral observations with known material profiles. Most objects in the solar system—asteroids, comets, planets, moons—are too cold to be interrogated in this manner. Material clouds around cold objects consist primarily of volatiles, so bulk composition cannot be probed. Additionally, low volatile density does not produce discernible absorption lines in the faint signal generated by cold objects. We propose a system for probing the molecular composition of cold solar system targets from a distant vantage. The concept utilizes a directed energy beam to melt and vaporize a spot on a distant target, such as from a spacecraft orbiting the object. With sufficient flux (~10 MW/m2) on a rocky asteroid, the spot temperature rises rapidly to ~2500 K, and evaporation of all materials on the surface occurs. The melted spot creates a high-temperature blackbody source, and ejected material creates a molecular plume in front of the spot. Bulk composition is investigated by using a spectrometer to view the heated spot through the ejected material. Spatial composition maps could be created by scanning the surface. Applying the beam to a single spot continuously produces a borehole, and shallow sub-surface composition profiling is possible. Initial simulations of absorption profiles with laser heating show great promise for molecular composition analysis

Simulations of Directed Energy Thrust on Rotating Asteroids

Asteroids that threaten Earth could be deflected from their orbits using directed energy to vaporize the surface, as the ejected plume creates a reaction thrust that alters the asteroid’s trajectory. In this situation, a critical issue is the rotation of the asteroid relative to the directed energy beam, as this will reduce the average thrust magnitude and modify the thrust direction. Flux levels required to evaporate surface material depend on the surface material composition, rotation rate, albedo, and thermal and bulk mechanical properties of the asteroid. The observed distribution of asteroid rotation rates is used, along with an estimated range of material and mechanical properties, as input to a 4D thermalphysical model to calculate the resultant thrust vector. The model uses a directed energy beam, striking the surface of a rotating sphere with specified material properties, beam profile, and rotation rate. The model calculates thermal changes in the sphere, including vaporization and mass ejection of the target material. The amount of vaporization integrated over the target is used to determine the thrust magnitude and the phase shift relative to the non-rotating case. As the object rotates beneath the beam, the energy spreads out, decreasing temperature and vaporization causing both a phase shift and magnitude decrease in the average thrust vector. This produces a 4D analytical model of the expected thrust profile for rotating objects