Analysis of Air Force Contingency Contracting Training Needs

This research examines the Air Force training needs of contingency contracting officers (CCOs). The study utilized the deductive approach to research. A survey instrument captured the data for the study. The survey captured input from CCOs with deployment experience and each of the Air Force components and MAJCOMs. Series 1 of the survey polls CCOs with deployment experience to determine the training CCOs require based on their personal experiences. Series 2 surveys the component and MAJCOM level supervisors to determine their perception of the training that should be required for CCOs. Comparing the two series will identify any differences in responses from the groups. Descriptive and analytical statistics were used to interpret the completed surveys. The survey will be used to determine what tasks should be trained prior to a CCO being deployed. This study tries to capture the general contracting tasks that may be performed at any contingency location. The survey results were combined and a set of training tasks were identified for CCOs

Perchlorate-specific proteomic stress responses of Debaryomyces hansenii could enable microbial survival in Martian brines

If life exists on Mars, it would face several challenges including the presence of perchlorates, which destabilize biomacromolecules by inducing chaotropic stress. However, little is known about perchlorate toxicity for microorganisms on the cellular level. Here, we present the first proteomic investigation on the perchlorate-specific stress responses of the halotolerant yeast Debaryomyces hansenii and compare these to generally known salt stress adaptations. We found that the responses to NaCl and NaClO4-induced stresses share many common metabolic features, for example, signalling pathways, elevated energy metabolism, or osmolyte biosynthesis. Nevertheless, several new perchlorate-specific stress responses could be identified, such as protein glycosylation and cell wall remodulations, presumably in order to stabilize protein structures and the cell envelope. These stress responses would also be relevant for putative life on Mars, which—given the environmental conditions—likely developed chaotropic defence strategies such as stabilized confirmations of biomacromolecules or the formation of cell clusters.Peer Reviewe

A MegaCam Survey of Outer Halo Satellites. VII. A Single S\'ersic Index v/s Effective Radius Relation for Milky Way Outer Halo Satellites

In this work we use structural properties of Milky Way's outer halo ($R_G > 25\,\mathrm{kpc}$) satellites (dwarf spheroidal galaxies, ultra-faint dwarf galaxies and globular clusters) derived from deep, wide-field and homogeneous data, to present evidence of a correlation in the S\'ersic index v/s effective radius plane followed by a large fraction of outer halo globular clusters and satellite dwarf galaxies. We show that this correlation can be entirely reproduced by fitting empirical relations in the central surface brightness v/s absolute magnitude and S\'ersic index v/s absolute magnitude parameter spaces, and by assuming the existence of two types of outer halo globular clusters: one of high surface brightness (HSB group), with properties similar to inner halo clusters; and another of low surface brightness (LSB group), which share characteristics with dwarf spheroidal and ultra-faint dwarf galaxies. Given the similarities of LSB clusters with dwarf spheroidal and ultra-faint dwarf galaxies, we discuss the possibility that outer halo clusters also originated inside dark matter halos and that tidal forces from different galaxy host's potentials are responsible for the different properties between HSB and LSB clusters.Comment: 20 pages, 9 figures, 3 table

Genome sequence data of Bacillus velezensis BP1.2A and BT2.4

Here, we report the complete genome sequence data of the biocontrol strains Bacillus velezensis BP1.2A and BT2.4 isolated from Vietnamese crop plants. The size of the genomes is 3,916,868 bp (BP1.2A), and 3,922,686 bp (BT2.4), respectively. The BioProjects have been deposited at NCBI GenBank. The GenBank accession numbers for the B. velezensis strains are PRJNA634914 (BP1.2A) and PRJNA634832 (BT2.4) for the BioProjects, CP085504 (BP1.2A) and CP085505 (BT2.4) for the chromosomes, GCA_013284785.2 (BP2.1A), and GCA_013284785.2 (BT2.4) for GenBank assembly accessions, and SAMN15012571 (BP1.2A) and SAMN15009897 (BT2.4) for the BioSamples. Both genomes were closely related to FZB42, the model strain for plant growth promoting bacilli.Peer Reviewe

Investigation of the potential of Brevibacillus spp. for the biosynthesis of nonribosomally produced bioactive compounds by combination of genome mining with MALDI-TOF mass spectrometry

The biosynthetic potential of 11 Brevibacillus spp. strains was investigated by combination of genome mining with mass spectrometric analysis using MALDI-TOF mass spectrometry. These endophytic, plant associated Brevibacillus strains were isolated from crop plants, such as coffee and black pepper, in Vietnam. Draft genomes of these strains were available. They were classified (a) by comparison with type strains and a collection of genome-sequenced Brevibacillus spp. deposited in the NCBI data base as well as (b) by construction of a phylogenetic tree from the core sequences of publicly available genomes of Brevibacillus strains. They were identified as Brevibacillus brevis (1 strain); parabrevis (2 strains); porteri (3 strains); and 5 novel Brevibacillus genomospecies. Our work was specifically focused on the detection and characterization of nonribosomal peptides produced by these strains. Structural characterization of these compounds was performed by LIFT-MALDI-TOF/TOF mass spectrometric sequence analysis. The highlights of our work were the demonstration of the tyrocidines, a well-known family of cyclodecapeptides of great structural variability, as the main products of all investigated strains and the identification of a novel class of pentapeptides produced by B. brevis; B. schisleri; and B. porteri which we designate as brevipentins. Our biosynthetic studies demonstrate that knowledge of their biosynthetic capacity can efficiently assist classification of Brevibacillus species.Peer Reviewe

Fire, late frost, nun moth and drought risks in Germany's forests under climate change

Ongoing climate change affects growth and increases biotic and abiotic threats to Germany's forests. We analysed how these risks develop through the mid-century under a variety of climate change scenarios using the process-based forest model 4C. This model allows the calculation of indicators for fire danger, late frost risk for beech and oak, drought stress and nun moth risk. 4C was driven by a set of 4 simulations of future climate generated with the statistical model STARS and with 10 simulations of future climate based on EURO-CORDEX model simulations for the RCP2.6, RCP4.5 and RCP8.5 pathways. A set of about 70000 forest stands (Norway spruce, Scots pine, beech, oak, birch), based on the national forest inventory describing 98.4 % of the forest in Germany, was used together with data from a digital soil map. The changes and the range of changes were analysed by comparing results of a recent time period (1971–2005) and a scenario time period (2011–2045). All indicators showed higher risks for the scenario time period compared to the recent time period, except the late frost risk indicators, if averaged over all climate scenarios. The late frost risk for beech and oaks decreased for the main forest sites. Under recent climate conditions, the highest risk with regard to all five indicators was found to be in the Southwest Uplands and the northern part of Germany. The highest climate-induced uncertainty regarding the indicators for 2011–2045 is projected for the East Central Uplands and Northeast German Plain

ATR-FTIR spectroscopy reveals genomic loci regulating the tissue response in high fat diet fed BXD recombinant inbred mouse strains

Background: Obesity-associated organ-specific pathological states can be ensued from the dysregulation of the functions of the adipose tissues, liver and muscle. However, the influence of genetic differences underlying gross-compositional differences in these tissues is largely unknown. In the present study, the analytical method of ATR-FTIR spectroscopy has been combined with a genetic approach to identify genetic differences responsible for phenotypic alterations in adipose, liver and muscle tissues. Results: Mice from 29 BXD recombinant inbred mouse strains were put on high fat diet and gross-compositional changes in adipose, liver and muscle tissues were measured by ATR-FTIR spectroscopy. The analysis of genotype-phenotype correlations revealed significant quantitative trait loci (QTL) on chromosome 12 for the content of fat and collagen, collagen integrity, and the lipid to protein ratio in adipose tissue and on chromosome 17 for lipid to protein ratio in liver. Using gene expression and sequence information, we suggest Rsad2 (viperin) and Colec11 (collectin-11) on chromosome 12 as potential quantitative trait candidate genes. Rsad2 may act as a modulator of lipid droplet contents and lipid biosynthesis; Colec11 might play a role in apoptopic cell clearance and maintenance of adipose tissue. An increased level of Rsad2 transcripts in adipose tissue of DBA/2J compared to C57BL/6J mice suggests a cis-acting genetic variant leading to differential gene activation. Conclusion: The results demonstrate that the analytical method of ATR-FTIR spectroscopy effectively contributed to decompose the macromolecular composition of tissues that accumulate fat and to link this information with genetic determinants. The candidate genes in the QTL regions may contribute to obesity-related diseases in humans, in particular if the results can be verified in a bigger BXD cohort

Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1

Paenibacilli are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnology. Lipopeptides produced by such organisms play a major role in their potential to inactivate pathogens. In this work we investigated two lipopeptide complexes, the fusaricidins and the polymyxins, produced by Paenibacillus polymyxa strains DSM 32871 and M1 by MALDI-TOF mass spectrometry. The fusaricidins show potent antifungal activities and are distinguished by an unusual variability. For strain DSM 32871 we identified numerous yet unknown variants mass spectrometrically. DSM 32871 produces polymyxins of type E (colistins), while M1 forms polymyxins P. For both strains, novel but not yet completely characterized polymyxin species were detected, which possibly are glycosylated. These compounds may be of interest therapeutically, because polymyxins have gained increasing attention as last-resort antibiotics against multiresistant pathogenic Gram-negative bacteria. In addition, the volatilomes of DSM 32781 and M1 were investigated with a GC–MS approach using different cultivation media. Production of volatile organic compounds (VOCs) was strain and medium dependent. In particular, strain M1 manifested as an efficient VOC-producer that exhibited formation of 25 volatiles in total. A characteristic feature of Paenibacilli is the formation of volatile pyrazine derivatives.Peer Reviewe

Draft Genome Sequence of Burkholderia puraquae Type Strain CAMPA 1040, Isolated from Hospital Settings in Córdoba, Argentina

We report here the draft genome sequence of Burkholderia puraquae type strain CAMPA 1040, a member of the Burkholderia cepacia complex. This strain, isolated from a hemodialysis water reservoir, harbors several stress tolerance genes, such as the systems for low oxygen survival, for copper tolerance, and for osmotic stress resistance.Facultad de Ciencias Exacta

Towards a Critical Understanding of Music, Emotion and Self-Identity

The article begins by outlining a dominant conception of these relations in sociologically informed analysis of music, which sees music primarily as a positive resource for active self-making. My argument is that this conception rests on a problematic notion of the self and also on an overly optimistic understanding of music, which implicitly sees music as highly independent of negative social and historical processes. I then attempt to construct a) a more adequately critical conception of personal identity in modern societies; and b) a more balanced appraisal of music-society relations. I suggest two ways in which relations between self, music and society may not always be quite so positive or as healthy as the dominant conception suggests: 1) Music is now bound up with the incorporation of authenticity and creativity into capitalism, and with intensified consumption habits. 2) Emotional self-realisation through music is now linked to status competition. Interviews are analysed