Use of different RT-QuIC substrates for detecting CWD prions in the brain of Norwegian cervids

Chronic wasting disease (CWD) is a highly contagious prion disease affecting captive and free-ranging cervid populations. CWD has been detected in United States, Canada, South Korea and, most recently, in Europe (Norway, Finland and Sweden). Animals with CWD release infectious prions in the environment through saliva, urine and feces sustaining disease spreading between cervids but also potentially to other non-cervids ruminants (e.g. sheep, goats and cattle). In the light of these considerations and due to CWD unknown zoonotic potential, it is of utmost importance to follow specific surveillance programs useful to minimize disease spreading and transmission. The European community has already in place specific surveillance measures, but the traditional diagnostic tests performed on nervous or lymphoid tissues lack sensitivity. We have optimized a Real-Time Quaking-Induced Conversion (RT-QuIC) assay for detecting CWD prions with high sensitivity and specificity to try to overcome this problem. In this work, we show that bank vole prion protein (PrP) is an excellent substrate for RT-QuIC reactions, enabling the detection of trace-amounts of CWD prions, regardless of prion strain and cervid species. Beside supporting the traditional diagnostic tests, this technology could be exploited for detecting prions in peripheral tissues from live animals, possibly even at preclinical stages of the disease

A comprehensive overview of radioguided surgery using gamma detection probe technology

The concept of radioguided surgery, which was first developed some 60 years ago, involves the use of a radiation detection probe system for the intraoperative detection of radionuclides. The use of gamma detection probe technology in radioguided surgery has tremendously expanded and has evolved into what is now considered an established discipline within the practice of surgery, revolutionizing the surgical management of many malignancies, including breast cancer, melanoma, and colorectal cancer, as well as the surgical management of parathyroid disease. The impact of radioguided surgery on the surgical management of cancer patients includes providing vital and real-time information to the surgeon regarding the location and extent of disease, as well as regarding the assessment of surgical resection margins. Additionally, it has allowed the surgeon to minimize the surgical invasiveness of many diagnostic and therapeutic procedures, while still maintaining maximum benefit to the cancer patient. In the current review, we have attempted to comprehensively evaluate the history, technical aspects, and clinical applications of radioguided surgery using gamma detection probe technology

Towards a Model of Transformation: Manager's Perceptions of Transformation in an E-business Environment

In the Entrepreneurial Millennium, companies and industries must come to terms with change. When change is radical, they must manage a metamorphosis by way of transformation. Here industry structures and relationships may change radically where new rules and guidelines are needed to manage the business and its customers in the transformed dimension. Some studies have conducted research and analysed findings according to the transformational impact on the organisations showing that early adopters of e-business show a trend towards cost reductions and administrative efficiencies while more mature users focus on strategic level change and advantage (Ash and Burn, 2003). Other researchers have studied the challenges that major organisations have encountered while expanding their ebusiness transformation strategy (Ranganathan, Shetty and Muthukumaran, 2004).Managers therefore need to develop a better understanding of the theory and processes associated with the transformation in an e-business context. This paper reviews the findings of field research and uses it as a basis to propose a new model of transformation which may guide future research and lead to a better understanding of what is involved in the process."Place" to "Space" transformation, transformation models, transformation process, electronic business, electronic business enablement, electronic business transformation, marketing channel innovation