Tetanus-diphtheria vaccine can prime SARS-CoV-2 cross-reactive T cells

Vaccines containing tetanus-diphtheria antigens have been postulated to induce cross-reactive immunity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which could protect against coronavirus disease (COVID-19). In this work, we investigated the capacity of Tetanus-diphtheria (Td) vaccine to prime existing T cell immunity to SARS-CoV-2. To that end, we first collected known SARS-CoV-2 specific CD8+ T cell epitopes targeted during the course of SARS-CoV-2 infection in humans and identified as potentially cross-reactive with Td vaccine those sharing similarity with tetanus-diphtheria vaccine antigens, as judged by Levenshtein edit distances (≤ 20% edits per epitope sequence). As a result, we selected 25 potentially cross-reactive SARS-CoV-2 specific CD8+ T cell epitopes with high population coverage that were assembled into a synthetic peptide pool (TDX pool). Using peripheral blood mononuclear cells, we first determined by intracellular IFNγ staining assays existing CD8+ T cell recall responses to the TDX pool and to other peptide pools, including overlapping peptide pools covering SARS-CoV-2 Spike protein and Nucleocapsid phosphoprotein (NP). In the studied subjects, CD8+ T cell recall responses to Spike and TDX peptide pools were dominant and comparable, while recall responses to NP peptide pool were less frequent and weaker. Subsequently, we studied responses to the same peptides using antigen-inexperienced naive T cells primed/stimulated in vitro with Td vaccine. Priming stimulations were carried out by co-culturing naive T cells with autologous irradiated peripheral mononuclear cells in the presence of Td vaccine, IL-2, IL-7 and IL-15. Interestingly, naive CD8+ T cells stimulated/primed with Td vaccine responded strongly and specifically to the TDX pool, not to other SARS-CoV-2 peptide pools. Finally, we show that Td-immunization of C57BL/6J mice elicited T cells cross-reactive with the TDX pool. Collectively, our findings support that tetanus-diphtheria vaccines can prime SARS-CoV-2 cross-reactive T cells and likely contribute to shape the T cell responses to the virus

An Integer Precise Point Positioning technique for sea surface observations using a GPS buoy

GPS data dedicated to sea surface observation are usually processed using differential techniques. Unfortunately, the precision of resulting kinematic positions is baseline-length dependent. So, high precision sea surface observations using differential GPS techniques are limited to coasts, lakes, and rivers. Recent improvements in GPS satellite products (orbits, clocks, and phase biases) make phase ambiguity fixing at the zero difference level achievable and opens up the observation of the sea surface without geographical constraints. This paper recalls the concept of the Integer Precise Point Positioning technique and discusses the precision of GPS buoy positioning. A sequential version of the GINS software has been implemented to achieve single epoch GPS positioning. We used 1 Hz data from a two week GPS campaign conducted in the Kerguelen Islands. A GPS buoy has been moored close to a radar gauge and 90 m away from a permanent GPS station. This infrastructure offers the opportunity to compare both kinematic Integer Precise Point Positioning and classical differential GPS positioning techniques to in situ radar gauge data. We found that Precise Point Positioning results are not significantly biased with respect to radar gauge data and that horizontal time series are consistent with differential processing at the sub-centimetre precision level. Nevertheless, standard deviations of height time series with respect to radar gauge data are typically [4-5] cm. The dominant driver for noise at this level is attributed to errors in tropospheric estimates which propagate into position solutions

Hydrological Loading in Western Europe Estimated from the Inversion of GPS Displacements computed with GINS/PC software

International audienceSince the launch of the GRACE satellites in 2002, followed by GRACE-FO in 2018, the determination of seasonal hydrological variations has been significantly improved. These surface mass variations also induce crustal deformation which can be measured by GNSS permanent stations. In this study, we produce a dense GPS solution in Western Europe using the GINS/PC software developed by CNES. We compare the solutions of Precise Point Positioning processing with or without fixing the integer ambiguities. Displacements can be linked to mass loading process using the Green's functions formalism, assuming an elastic or anelastic Earth models. The observed displacements are then inverted in order to recover hydrological seasonal variations. We use a regularized least square inversion for vertical and horizontal displacements and compare our estimations to GRACE solutions and global hydrological models. We show that dense and homogeneous GPS networks allow a retrieval of mass variations with spatial resolution equivalent or even better than GRACE

On the mechanical behaviour of additively manufactured metamaterials under dynamic conditions

High-energy absorption and light-weightiness are two critical properties for impact protection in the aerospace sector. In the past, the use of periodic honeycomb structures or random porous metallic foams were the preferred route to obtain a good specific-energy absorption performance. In recent years, the use of additive manufacturing has increased the design freedom creating a new generation of reticulated and porous materials: the metamaterials or lattice materials. The internal geometries of these lattice structures can be tuned for superior optimal properties, e.g., energyabsorption and density. However, the mechanics of these materials under impact need to be understood with the purpose of mechanical optimisation, and the computational models validated. In this work, we present the experimental compressive behaviour, at room temperature, of two Ti6Al4V lattice structures under static and dynamic conditions. The quasi-static tests were performed by using a universal testing machine while the dynamic tests were conducted at 480s-1 with a split-Hopkinson bar. In all cases, the deformation process was filmed to analyse the failure. Finally, finiteelement simulations were done, employing the Johnson-Cook model, to describe the response of the alloy. The simulations were able to reflect the failure characteristics of each metamaterial but were not able to describe the macroscopic response due to the differences between the experimental and computational volume fraction

Vibrations of Mertz Glacier ice tongue, East Antarctica

At the time of its calving in February 2010, Mertz Glacier, East Antarctica, was characterized by a 145km long, 35km wide floating tongue. In this paper, we use GPS data from the Collaborative Research into Antarctic Calving and Iceberg Evolution (CRAC-ICE) 2007/08 and 2009/10 field seasons to investigate the dynamics of Mertz Glacier. Twomonths of data were collected at the end of the 2007/08 field season from two kinematic GPS stations situated on each side of the main rift of the glacier tongue and from rock stations located around the ice tongue during 2008/09. Using Precise Point Positioning with integer ambiguity fixing, we observe that the two GPS stations recorded vibrations of the ice tongue with several dominant periods. We compare these results with a simple elastic model of the ice tongue and find that the natural vibration frequencies are similar to those observed. This information provides a better understanding of their possible effects on rift propagation and hence on the glacier calving processes

Impact of loading phenomena on velocity field computation from GPS campaings : Application to the ResPyr GPS Campaign in the Pyrenees

International audienc

An absolute calibration site for radar altimeters in the continental domain : lake Issykkul in Central Asia

Altimetry missions such as Topex/Poseidon, Jason-1, GFO and ENVISAT have been widely used in the continental domain over lakes, rivers and wetland although they were mostly dedicated to oceanic studies. Knowledge of the instrumental biases is a key issue. Numerous sites have been dedicated to calibration purposes, either in the oceanic domain (Harvest offshore platform in California, Corsica, Bass Strait in Australia) or over lakes (Lake Erie in United States). A new site (Lake Issykkul in Kirghizstan) is proposed for calibration in the continental domain. This lake is covered by past (T/P) and current radar altimetry satellites (Jason-1, T/P, GFO, and ENVISAT). Several in situ water levels and local meteorological variables are available at the site. Located in a mountainous area, it offers an opportunity for calibration far away from all other existing sites and very different environment contexts. Two GPS campaigns have been conducted on the lake in 2004 and in 2005. They consisted of cruises with stations installed onboard a boat following the satellite ground tracks, and onshore settings. This enabled estimating a bias for each altimeter and each tracking algorithm available. Biases obtained for Envisat, GFO, T/P and Jason-1 using the default ocean tracker (respectively, 48.1 +/- 6.6, 7.5 +/- 4.0, 0 +/- 4.3 and 7.0 +/- 5.5 cm) agree with biases published at the other calibration sites. For Jason-1, there is a significant disagreement with results obtained in the ocean field (7 cm instead of 13 cm) but is coherent with bias obtained on the Lake Erie site. Erroneous estimates of the sea state bias correction from non-oceanic-like waveforms is discussed as a possible explanation. Errors in the ionospheric, wet and dry tropospheric corrections for the continental domain are also highlighted and quantified