84 research outputs found

    Assessment of the potential in vivo ecotoxicity of Double-Walled Carbon Nanotubes (DWNTs) in water, using the amphibian Ambystoma mexicanum

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    Because of their specific properties (mechanical, electrical, etc), carbon nanotubes (CNTs) are being assessed for inclusion in many manufactured products. Due to their massive production and number of potential applications, the impact of CNTs on the environment must be taken into consideration. The present investigation evaluates the ecotoxic potential of CNTs in the amphibian larvae (Ambystoma mexicanum). Acute toxicity and genotoxicity were analysed after 12 days of exposure in laboratory conditions. The genotoxic effects were analysed by scoring the micronucleated erythrocytes in the circulating blood of the larvae according to the French standard micronucleus assay. The results obtained in the present study demonstrated that CNTs are neither acutely toxic nor genotoxic to larvae whatever the CNTs concentration in the water, although black masses of CNTs were observed inside the gut. In the increasing economical context of CNTs, complementary studies must be undertaken, especially including mechanistic and environmental investigations

    Prevalence and significance of sexually transmitted diseases among Ethiopian women attending antenatal clinics in Addis Ababa

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    Abstract: To determine the prevalence of sexually transmitted diseases (STDs) and the risk for (i) the mother regarding pregnancy wastage and puerperal sepsis and (ii) the child with regard to congenital and neonatal infection, 342 routine antenatal clinic (ANC) at tenders were investigated. The prevalence of antibodies showing exposure to specific STD pathogens in pregnant women attending ANC was: syphilis (TPHA) 27%, (VDR:) 28%, gonorrhoea 43%, genital chlamydiae 54%, HBV 37%, HSV-2 35 %, H ducreyi 10%. High titre seropositivity suggestive of active infection was: gonorrhoea 10%, genital chlamydiae 31 %, HSV2 19%; with HBV SAg 5% -all of which are likely to be transmitted to the foetus in utero or during delivery. Only 10% of ANC at tenders had no serological evidence of any STD: 72% had serological evidence for two or more STDs. Among conditions requiring treatment vaginitis was the most important, 20% having a severe trichomonal infection. Despite the frequency of this condition it was noted that few women (4%) complained of vaginal discharge. Thus women attending the ANC revealed a high prevalence of STD. Consequently the foetus and neonate are put at risk because of intrauterine or intrapartum transmission of infection. The high prevalence among ANC at tenders also reflects the relative prevalence of STDs in the community. Measures such as screening at ANC and information and education regarding prevention are required to reduce STDs in pregnant women and their sexual partners. Prophylaxis for the neonate can be considered until this goal is achieved. [Ethiop. J. Health Dev. 1995;9(1):31-40

    A sociological and serological study of at tenders of family planning clinics in Addis Ababa

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    Abstract: A study of 542 women attending family planning clinics (FPC) and 1568 women attending obstetric and gynaecologic clinics in Addis Ababa showed utilisation of FPC was highest in those with a family income of 100-500 EB per month (36%), in women who were: Tigrawi (33%) or Amara (31 %), aged 20-34 years (30%), age 16 or older at first marriage/coitus (28%), parity of 2... 2 children (35%), > 5 lifetime husbands/sexual partners (39%), or were bargirls (73%) or prostitutes (43%). FPC attendance was lowest among the nulliparous (2.3%), women from rural areas (10%), the Guragie (10%) and Oromo women (19%), Moslem women 14(%), those of subsistence income ( < 10EB per month) (14%). The seroprevalence rates indicative of exposure to STD pathogens were high as was the prevalence of essentially asymptomatic pelvic inflammatory disease (PID). Only 4% of FPC at tenders had no serological evidence of STD: 64% had 3 or more different STD. Specific present or active STD infection prevalence for syphilis (VDRL) 28%, Neisseria gonorrhoea 31 %, genital chlamydia 46% and HSV-2 21% was higher in FPC at tenders than among women attending other clinics. Clinical evidence of PID was also more common in the FPC at tenders (54%), 37% having evidence of salpingitis. Thus FPCs provide a useful setting for screening women particularly at risk. Because of lack of symptoms, these women are unlikely to attend either an STDs clinic or a hospital for routine check up, and as such are not treated and represent a population from which STDs can spread into the population. Measures to screen, treat and educate FPC at tenders, their partners and their clients, are recommended in an attempt to Control STDs and ultimately HIV in the community. [Ethiop. J. Hea/th Dev. 1995;9(1):19-30

    Water dispersible microbicidal cellulose acetate phthalate film

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    BACKGROUND: Cellulose acetate phthalate (CAP) has been used for several decades in the pharmaceutical industry for enteric film coating of oral tablets and capsules. Micronized CAP, available commercially as "Aquateric" and containing additional ingredients required for micronization, used for tablet coating from water dispersions, was shown to adsorb and inactivate the human immunodeficiency virus (HIV-1), herpesviruses (HSV) and other sexually transmitted disease (STD) pathogens. Earlier studies indicate that a gel formulation of micronized CAP has a potential as a topical microbicide for prevention of STDs including the acquired immunodeficiency syndrome (AIDS). The objective of endeavors described here was to develop a water dispersible CAP film amenable to inexpensive industrial mass production. METHODS: CAP and hydroxypropyl cellulose (HPC) were dissolved in different organic solvent mixtures, poured into dishes, and the solvents evaporated. Graded quantities of a resulting selected film were mixed for 5 min at 37°C with HIV-1, HSV and other STD pathogens, respectively. Residual infectivity of the treated viruses and bacteria was determined. RESULTS: The prerequisites for producing CAP films which are soft, flexible and dispersible in water, resulting in smooth gels, are combining CAP with HPC (other cellulose derivatives are unsuitable), and casting from organic solvent mixtures containing ≈50 to ≈65% ethanol (EtOH). The films are ≈100 µ thick and have a textured surface with alternating protrusions and depressions revealed by scanning electron microscopy. The films, before complete conversion into a gel, rapidly inactivated HIV-1 and HSV and reduced the infectivity of non-viral STD pathogens >1,000-fold. CONCLUSIONS: Soft pliable CAP-HPC composite films can be generated by casting from organic solvent mixtures containing EtOH. The films rapidly reduce the infectivity of several STD pathogens, including HIV-1. They are converted into gels and thus do not have to be removed following application and use. In addition to their potential as topical microbicides, the films have promise for mucosal delivery of pharmaceuticals other than CAP

    Escutando as mães de portadores de fissuras orais

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    Estudo descritivo com abordagem qualitativa. Objetivou a escuta e o relato sobre a etiologia da malformação e conhecer as crenças atribuídas pelos familiares à manifestação das fissuras orais. Os dados foram coletados de março a junho de 2009 por entrevista semi-estruturada e analisados por análise de conteúdo. Resultou em cinco categorias: reação da mãe, sentimentos das mães e da família, comportamento da equipe, conhecimento da causa e crenças. Os resultados permitiram conhecer as crenças sobre o tema e adequar as orientações da enfermagem e dos demais profissionais às mães de portadores de fissuras orais, auxiliando os familiares em todas as fases de desenvolvimento da criança durante o processo terapêutico

    The interaction of bacterial pathogens with platelets.

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    In recent years, the frequency of serious cardiovascular infections such as endocarditis has increased, particularly in association with nosocomially acquired antibiotic-resistant pathogens. Growing evidence suggests a crucial role for the interaction of bacteria with human platelets in the pathogenesis of cardiovascular infections. Here, we review the nature of the interactions between platelets and bacteria, and the role of these interactions in the pathogenesis of endocarditis and other cardiovascular diseases

    Addressing tobacco in Australian alcohol and other drug treatment settings: a cross-sectional survey of staff attitudes and perceived barriers

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    Background: Within alcohol and other drug (AOD) services, staff attitudes and beliefs are important influences determining provision of smoking cessation care. This study of AOD staff aimed to examine: a) current attitudes toward smoking cessation care; b) service and staff characteristics associated with unsupportive smoking cessation care attitudes, and c) perceived barriers to providing smoking cessation care. Methods: Between July-October 2014, 506 staff from 31 Australian AOD services completed an online cross-sectional survey which assessed agreement with 6 attitudinal statements (supportive and unsupportive) and 10 perceived barriers to smoking cessation care in the AOD setting. Logistic regressions examined service (sector) and staff (age, gender, smoking status and number of years in AOD field) characteristics associated with unsupportive smoking cessation care attitudes. Results: A large proportion agreed with supportive statements: Smoking cessation care should be part of usual care (87%), smoking cessation care is as important as counselling about other drugs (72%) and staff have the organisational support to provide smoking cessation care (58%). Some respondents agreed with unsupportive statements: AOD clients are not interested in addressing their smoking (40%), increasing smoking restrictions would lead to client aggression (23%), smoking is a personal choice and it is not the service’s role to interfere (16%). Respondents from non-government managed services, current tobacco smokers (compared to ex-smokers) and those with less AOD experience had higher odds of agreeing with unsupportive smoking cessation care statements. The most frequently identified barriers to providing smoking cessation care were: client inability to afford cessation medicines, insufficient funding and lack of a coordinated treatment approach (all 61%). Conclusions: Overall, staff hold largely supportive smoking cessation care attitudes but perceive a large number of barriers to providing smoking cessation care
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