Sistemas de autoevaluación virtual en inglés para fomentar el plurilingüismo en la asignatura de reproducción y obstetricia

In this project, training clips showing practical activities of Reproduction and Obstetrics has been developed. These videos contain English audio and text and can be used to complete teaching presentations, making the learning process easier. Moreover, training clips also include questionnaires, which promote self-evaluation of the students and the search of external resources. All these contribute to obtain an important tool within a multilingualism program for teaching.En el presente proyecto se han elaborado de videos demostrativos de diferentes aspectos prácticos de la asignatura Reproducción y Obstetricia (“training clips”) con audio en inglés que completan las presentaciones, facilitando el aprendizaje de los estudiantes. Asimismo, estos videos forman parte de una plataforma de enseñanza interactiva, que incluye cuestionarios que permiten la búsqueda de información y autoevaluación del alumno. Esto permite disponer de una herramienta didáctica aplicable dentro de un marco de docencia plurilingüe

The Effect of different vitrification and staining protocols on the visibility of the nuclear maturation stage of equine oocytes

In this study, we compared two staining protocols assessing the nuclear chromatin stage of equine oocytes after vitrification using permeable and nonpermeable cryoprotectants. Slaughterhouse-derived oocytes (n = 155) were obtained from a total of 32 mares and in vitro matured in M199 medium for 42 hours at 38.5°C in 5% CO2. In the first experiment, two concentrations of Hoechst 33342 (HO) were tested (10 μg/mL; P1 and 2.5 μg/mL; P2) combined with 50 μg/mL of propidium iodide as staining protocols to evaluate the visibility of matured oocytes (n = 44). In the second experiment, 111 oocytes were evaluated using the staining protocol P2, before (C, control) and after vitrification following a two-step conventional protocol with (15% dimethyl sulfoxide, 15% ethylene glycol, and 0.5 M sucrose; V1) or without (1 M sucrose; V2) using permeable cryoprotectants. Our results showed that P2 provided a higher percentage of oocytes with outstanding visibility of the nuclear chromatin stage (52.17%; P < .05) in comparison with P1 (19.04%). In the second experiment, no cryoprotectant-free vitrified oocytes reached the metaphase II maturation stage. This result was significantly lower (P < .05) than conventional vitrification (15.38%) and both lower in comparison with the nonvitrified control group (42.11%). In conclusion, permeable cryoprotectant-free vitrification of equine oocytes obtained poor results and therefore cannot be considered an alternative to vitrification using permeable cryoprotectants. In addition, a staining protocol with a low concentration of HO is recommended to evaluate the nuclear chromatin stage of equine oocytes after in vitro maturation.Fil: Pereira, Blasa C.. Universidad de Córdoba; EspañaFil: Ortiz, Isabel. Universidad de Córdoba; EspañaFil: Dorado, Jesús. Universidad de Córdoba; EspañaFil: Diaz Jimenez, Maria. Universidad de Córdoba; EspañaFil: Consuegra, Cesar. Universidad de Córdoba; EspañaFil: Demyda Peyrás, Sebastián. Universidad Nacional de La Plata; Argentina. Universidad de Córdoba; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Hidalgo, Manuel. Universidad de Córdoba; Españ

Evaluation of DNA Damage of Mare Granulosa Cells Before and After Cryopreservation Using a Chromatin Dispersion Test

DNA fragmentation of granulosa cells might be related to developmental competence of the equine oocyte. Granulosa cells are commonly stored before DNA fragmentation assessment, but the effect of preservation methods on this parameter remains unexplored. The aim of this study was to evaluate whether or not cryopreservation of granulosa cells affects the DNA damage. Equine oocytes were recovered from postmortem ovaries of five mares. Granulosa cells were washed by centrifugation and then analyzed (control) or stored in cryovials following four different protocols: P1 = directly plunged in liquid nitrogen (LN2) and then stored at −80°C; P2 = LN2/−80°C adding cryoprotectants (7.5% ethylene glycol + 7.5% dimethyl sulfoxide); P3 = −80°C; P4 = −80°C + cryoprotectants. Granulosa cell samples were processed with the prototype D3-Ovoselect, Halotech DNA, Spain), and DNA was visualized under fluorescence microscopy. High, low, and total DNA fragmentation percentages were compared among treatments by analysis of variance. Results were expressed as mean ± standard error. No significant differences (P >.05) were found among treatments and the control group. Therefore, the four conservation protocols could be considered equally efficient for DNA preservation of granulosa cells from mare oocytes. In conclusion, cryopreservation of granulosa cells in any of the four protocols used adequately preserved the DNA for further analysis.Fil: Pereira, Blasa C.. Universidad de Córdoba; EspañaFil: Ortiz, Isabel. Universidad de Córdoba; EspañaFil: Dorado, Jesús. Universidad de Córdoba; EspañaFil: Consuegra, Cesar. Universidad de Córdoba; EspañaFil: Jiménez Díaz, María Atilana. Universidad de Córdoba; EspañaFil: Demyda Peyrás, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Gosalvez, Jaime. Universidad Autónoma de Madrid; EspañaFil: Hidalgo, Manuel. Universidad de Córdoba; Españ