38 research outputs found
What limits supercurrents in high temperature superconductors? A microscopic model of cuprate grain boundaries
The interface properties of high-temperature cuprate superconductors have
been of interest for many years, and play an essential role in Josephson
junctions, superconducting cables, and microwave electronics. In particular,
the maximum critical current achievable in high-Tc wires and tapes is well
known to be limited by the presence of grain boundaries, regions of mismatch
between crystallites with misoriented crystalline axes. In studies of single,
artificially fabricated grain boundaries the striking observation has been made
that the critical current Jc of a grain boundary junction depends exponentially
on the misorientation angle. Until now microscopic understanding of this
apparently universal behavior has been lacking. We present here the results of
a microscopic evaluation based on a construction of fully 3D YBCO grain
boundaries by molecular dynamics. With these structures, we calculate an
effective tight-binding Hamiltonian for the d-wave superconductor with a grain
boundary. The critical current is then shown to follow an exponential
suppression with grain boundary angle. We identify the buildup of charge
inhomogeneities as the dominant mechanism for the suppression of the
supercurrent.Comment: 28 pages, 12 figure
Oligo-DNA Custom Macroarray for Monitoring Major Pathogenic and Non-Pathogenic Fungi and Bacteria in the Phyllosphere of Apple Trees
BACKGROUND: To monitor the richness in microbial inhabitants in the phyllosphere of apple trees cultivated under various cultural and environmental conditions, we developed an oligo-DNA macroarray for major pathogenic and non-pathogenic fungi and bacteria inhabiting the phyllosphere of apple trees. METHODS AND FINDINGS: First, we isolated culturable fungi and bacteria from apple orchards by an agar-plate culture method, and detected 32 fungal and 34 bacterial species. Alternaria, Aureobasidium, Cladosporium, Rhodotorula, Cystofilobasidium, and Epicoccum genera were predominant among the fungi, and Bacillus, Pseudomonas, Sphingomonas, Methylobacterium, and Pantoea genera were predominant among the bacteria. Based on the data, we selected 29 major non-pathogenic and 12 phytopathogenic fungi and bacteria as the targets of macroarray. Forty-one species-specific 40-base pair long oligo-DNA sequences were selected from the nucleotide sequences of rDNA-internal transcribed spacer region for fungi and 16S rDNA for bacteria. The oligo-DNAs were fixed on nylon membrane and hybridized with digoxigenin-labeled cRNA probes prepared for each species. All arrays except those for Alternaria, Bacillus, and their related species, were specifically hybridized. The array was sensitive enough to detect 10(3) CFU for Aureobasidium pullulans and Bacillus cereus. Nucleotide sequencing of 100 each of independent fungal rDNA-ITS and bacterial 16S-rDNA sequences from apple tree was in agreement with the macroarray data obtained using the same sample. Finally, we analyzed the richness in the microbial inhabitants in the samples collected from apple trees in four orchards. Major apple pathogens that cause scab, Alternaria blotch, and Marssonina blotch were detected along with several non-phytopathogenic fungal and bacterial inhabitants. CONCLUSIONS: The macroarray technique presented here is a strong tool to monitor the major microbial species and the community structures in the phyllosphere of apple trees and identify key species antagonistic, supportive or co-operative to specific pathogens in the orchard managed under different environmental conditions
The influence of Thrips obscuratus on infection and contamination of kiwifruit by Botrytis cinerea
International audienc