1 research outputs found
MiRâ223â3p alleviates trigeminal neuropathic pain in the male mouse by targeting MKNK2 and MAPK/ERK signaling
Abstract Background Trigeminal neuralgia (TN) is a neuropathic pain that occurs in branches of the trigeminal nerve. MicroRNAs (miRNAs) have been considered key mediators of neuropathic pain. This study was aimed to elucidate the pathophysiological function and mechanisms of miRâ223â3p in mouse models of TN. Methods Infraorbital nerve chronic constriction injury (CCIâION) was applied in male C57BL/6J mice to establish mouse models of TN. Pain responses were assessed utilizing Von Frey method. The expression of miRâ223â3p, MKNK2, and MAPK/ERK pathway protein in trigeminal ganglions (TGs) of CCIâION mice was measured using RTâqPCR and Western blotting. The concentrations of inflammatory cytokines were evaluated using Western blotting. The relationship between miRâ223â3p and MKNK2 was tested by a luciferase reporter assay. Results We found that miRâ223â3p was downregulated, while MKNK2 was upregulated in TGs of CCIâION mice. MiRâ223â3p overexpression by an intracerebroventricular injection of LvâmiRâ223â3p attenuated trigeminal neuropathic pain in CCIâION mice, as well as reduced the protein levels of proâinflammatory cytokines in TGs of CCIâION mice. MKNK2 was verified to be targeted by miRâ223â3p. Additionally, miRâ223â3p overexpression decreased the phosphorylation levels of ERK1/2, JNK, and p38 protein in TGs of CCIâION mice to inhibit MAPK/ERK signaling. Conclusions Overall, miRâ223â3p attenuates the development of TN by targeting MKNK2 to suppress MAPK/ERK signaling