Study on the visible-light-induced photokilling effect of nitrogen-doped TiO2 nanoparticles on cancer cells

Nitrogen-doped TiO2 (N-TiO2) nanoparticles were prepared by calcining the anatase TiO2 nanoparticles under ammonia atmosphere. The N-TiO2 showed higher absorbance in the visible region than the pure TiO2. The cytotoxicity and visible-light-induced phototoxicity of the pure- and N-TiO2 were examined for three types of cancer cell lines. No significant cytotoxicity was detected. However, the visible-light-induced photokilling effects on cells were observed. The survival fraction of the cells decreased with the increased incubation concentration of the nanoparticles. The cancer cells incubated with N-TiO2 were killed more effectively than that with the pure TiO2. The reactive oxygen species was found to play an important role on the photokilling effect for cells. Furthermore, the intracellular distributions of N-TiO2 nanoparticles were examined by laser scanning confocal microscopy. The co-localization of N-TiO2 nanoparticles with nuclei or Golgi complexes was observed. The aberrant nuclear morphologies such as micronuclei were detected after the N-TiO2-treated cells were irradiated by the visible light

Effect of bevacizumab assisted PD-1 inhibitor on serum miR-20a-5p and miR-515-3p in the treatment of gastric cancer

Background and purpose: Second-line chemotherapy is not effective in the treatment of gastric cancer. Bevacizumab is a molecularly targeted anticancer drug, and sindilizumab is a domestic programmed death-1 (PD-1) inhibitor. The combination of the two is a new direction for clinical treatment of gastric cancer. This study aimed to analyze the effects of bevacizumab assisted PD-1 inhibitor in the treatment of gastric cancer on serum miR-20a-5p and miR-515-3p. Methods: A retrospective study was conducted on 84 patients with gastric cancer treated in the Fourth Affiliated Hospital of Nanjing Medical University from January 2019 to July 2021, and they were divided into the observation group and the control group with 42 cases each according to different treatment plans. The control group was given second-line chemotherapy, and the observation group was given bevacizumab combined with PD-1 inhibitor (sindilizumab) on the basis of the control group. objective response rate (ORR), disease control rate (DCR), serum tumor markers, immune function indexes, serum miR-20a-5p and miR-515-3p levels and total incidence of toxic and side effects were compared between the two groups. This study has been approved by the Ethics Committee of the Fourth Affiliated Hospital of Nanjing Medical University (approval number: 20230531--K061). Results: The ORR (69.05%) and DCR (85.71%) of the observation group were higher than those of the control group (40.48% and 64.29%), and the difference was statistically significant (P<0.05). After treatment, serum carbohydrate antigen 12-5 (CA12-5), carcinoembryonic antigen (CEA) and cytokerantin-19-fragment antigen 21-1 (CYFRA21-1) levels were lower in observation group than in control group (P<0.05). CD4+ T lymphocyte and CD4+/CD8+ T lymphocyte ratio were higher in observation group than in control group after treatment (P<0.05), and CD8+ T lymphocyte was lower in observation group than in control group after treatment (P<0.05). After treatment, the serum levels of miR-20a-5p and miR-515-3p were lower in the observation group than in the control group (P<0.05). There was no significant difference in total incidence of adverse effects between the observation group (28.57%) and the control group (38.10%) (P>0.05). Conclusion: Bevacizumab assisted sindilizumab can effectively improve the treatment efficiency of gastric cancer, improve immune function, and reduce the expression levels of serum miR-20a-5p, miR-515-3p and tumor markers, without increasing toxic and side effects

Perception of Threatening Intention Modulates Brain Processes to Body Actions: Evidence From Event-Related Potentials

Efficiently perceiving a threatening intention conveyed by others’ bodily actions has great survival value. The current study examined if the human brain is sensitive to differences in intentions that are conveyed via bodily actions. For this purpose, a new intention categorization task was developed in which participants sat in front of a computer screen on which the pictures of highly threatening (HT), moderately threatening (MT), and non-threatening (NT) body actions were presented randomly. Participants were asked to press the corresponding buttons using threatening intention judgment, while event-related potentials (ERPs) were recorded. According to a cluster permutation test, we analyzed N190, N2, EPP (early posterior positivity), and P3. The results showed there was a positive correlation between the amplitude of the EPP induced by three kinds of body actions and the reaction time of the task. The results also revealed that when the deflection of EPP was less positive, the reaction time was shorter. We suggest that EPP might be useful as an index of body intention processing of the brain. The current study revealed that intention perception of body actions modulates brain processing

The Statistical Polarization Properties of Coherent Curvature Radiation by Bunches: Application to Fast Radio Burst Repeaters

Fast radio bursts (FRBs) are extragalactic radio transients with millisecond duration and extremely high brightness temperature. Very recently, some highly circularly polarized bursts were found in a repeater, FRB 20201124A. The significant circular polarization might be produced by coherent curvature radiation by bunches with the line of sight (LOS) deviating from the bunch central trajectories. In this work, we carry out simulations to study the statistical properties of burst polarization within the framework of coherent curvature radiation by charged bunches in the neutron star magnetosphere for repeating FRBs. The flux is almost constant within the opening angle of the bunch. However, when the LOS derivates from the bunch opening angle, the larger the derivation, the larger the circular polarization but the lower the flux. We investigate the statistical distribution of circular polarization and flux of radio bursts from an FRB repeater, and find that most of the bursts with high circular polarization have a relatively low flux. Besides, we find that most of the depolarization degrees of bursts have a small variation in a wide frequency band. Furthermore, we simulate the polarization angle (PA) evolution and find that most bursts show a flat PA evolution within the burst phases, and some bursts present a swing of PA.Comment: 12 pages, 13 figures, accepted for publication in Ap

Study on a Novel Disphase Supplying Supported Liquid Membrane for Transport Behavior of Divalent Nickel Ions

Abstract A novel disphase supplying supported liquid membrane (DSSLM), containing supplying feed phase and supplying stripping phase for transport behavior of Ni(II), have been studied. The supplying supported feed phase included feed solution and di(2-ethyhexyl) phosphoric acid (HDEHP) as the carrier in kerosene, and supplying stripping phase included HDEHP as the carrier in kerosene and HCl as the stripping agent. The effects of volume ratio of membrane solution to feed solution (O/F), pH, initial concentration of Ni(II) and ionic strength in the feed solution, volume ratio of membrane solution to stripping solution (O/S), concentration of H 2 SO 4 solution, HDEHP concentration in the supplying stripping phase on transport of Ni(II), the advantages of DSSLM compared to the traditional supported liquid membrane (SLM), the system stability, the reuse of membrane solution and the retention of membrane phase were studied. Experimental results indicated that the optimum transport of Ni(II) was obtained when H 2 SO 4 concentration was 2.00 mol·

A High Performance Target Tracing Transmission Model Oriented to Lifecycle Maximization

For the high speed sensor networks applications such as Internet of Things, multimedia transmission, the realization of high-rate transmission under limited resources has become a problem to be solved. A high speed transmission and energy optimization model oriented to lifecycle maximization is proposed in this paper. Based on information-directed mechanism, the energy threshold set and the relay node distance selection will be done in the process of target tracing, as a result, retaining a balance between transmission rate and energy consumption. Meanwhile, multiagent coevolution is adopted to achieve the maximum of network lifecycle. Comparing with the relevant methods, indexes for network such as hops, throughput, and number of active nodes, standard deviation of remaining energy, and the network lifecycle are considered, and the simulated experiments show that the proposed method will promote the transmission rate effectively, prolong the network lifecycle, and improve network performance as a whole