Impact of the volume and the profile of volatile fatty acids in the rumen fermentation on cow productivity and milk composition

Cilj ovog rada bio je utvrditi odnose između odabranih produkata fermentacije u buragu i sastava mlijeka muznih krava. Istraživanje je provedeno na 10 krava pasmine holstein-friesian. Životinje su držane u slobodnom stajskom uzgoju i hranjene TMR-om. Produktivnost stada određena je pomoću A4 metode, a podaci o sastavu mlijeka dobiveni su iz centralnog sustava praćenja. Ferment iz buraga izuzet je pomoću želučane pumpe 2 sata nakon jutarnje hranidbe smjesom TMR. Izuzeti uzorci razrijeđeni su puferom i homogenizirani, a dobivena suspenzija je analizirana pomoću plinskog kromatografa opremljenog s FID detektorom kako bi se odredila koncentracija ukupnih, kao i udjeli pojedinačnih hlapivih masnih kiselina (VFA). Sastav mlijeka preuzet je iz zapisa sustava praćenja RW-1, a koeficijent korelacije prema Pearsonu je izračunat iz dobivenih parametara. Utvrđeno je da se odnos propionat/butirat u fermentu buraga povećava s povećanjem udjela proteina i proteinskih frakcija u mlijeku. Koeficijent iskoristivosti hlapivih masnih kiselina u fermentu buraga pokazao je pozitivnu korelaciju s udjelom proteina i negativnu korelaciju s koncentracijom kazeina u mlijeku. Procesi koji se odvijaju u kravljem buragu značajno utječu na sastav i kvalitetu mlijeka. Spoznaje o ukupnom sadržaju kao i sadržaju pojedinih hlapivih masnih kiselina omogućuju podešavanje upravljanja fermentacijom u buragu u svrhu dobivanja boljih tehnoloških svojstava mlijeka.The aim of the research was to establish relations between the selected rumen fermentation products and the milk composition in dairy cows. The experiment was conducted on 10 Polish Holstein-Friesian cows of black and white variety. The animals were kept in the free stall housing system and fed with the total mixed ration (TMR). The herd’s milk production was assessed using the A4 method and the milk analyses were obtained from the milk recordings. The rumen digesta were sampled two hours after the morning delivery of TMR using a stomach pump. The sampled digesta were diluted with buffer, homogenized and the obtained suspension was analysed using gas chromatograph with the FID detector to determine the total concentration of volatile fatty acids (VFA) and the percentage of individual VFAs. Milk composition was obtained from the RW-1 reports, and the Pearson correlation coefficient was calculated for the obtained parameters. It was determined that propionate and the relation of propionate to butyrate in the rumen digesta increased the protein content and its fractions in milk. The volatile fatty acids utilization coefficient in the rumen digesta a positive correlation to protein contents and a negative correlation to the level of caseins in milk were shown. Processes taking place in the cow rumen significantly influence the composition and quality of milk. The knowledge of volatile fatty acids content and the of individual VFAs allows for the management of the rumen fermentation in order to obtain better technological parameters of milk

Use of Corn Dried Distillers Grains (DDGS) in Feeding of Ruminants

Bioethanol is the product of fermentation of starch contained in renewable resources, such as corn, wheat, rye and rice. Depending on the technology used for its production, dried distillers decoction may exist in different forms: dried distillers grain (DDG); dried distillers grain with solubles (DDGS) and high-protein dried distillers grains (HPDDG), as well as wet distillers grain (WDG), wet distillers grain with solubles (WDGS), and high-protein wet distillers grains HPWDG). Research conducted in recent years has demonstrated the possibilities of corn DDG as feed for livestock due to its high content of valuable protein, high calorific value and bioelements. Distillers grain has been used as feed for beef and dairy cattle, sheep, swine and poultry. In case of ruminants, it is important that distillers grain is foodstuff high in ruminal undegradable protein, with beneficial fibre content that does not cause rumen acidosis. DDGS has positive influence on milk yield and its fat and protein content. Research on rumen fermentation has proven that DDGS positively affecs processes in forestomachs: methanogenesis, ammonia emission and volatile fatty acids profile. Reprocessing of agri-food industry by-products may well be an alternative for traditional methods of feeding animals and utilizing valuable nutrients that they contain

Utjecaj hranidbe na kvalitetu kolostruma i mlijeka preživača

The composition and properties of mammary glands’ secretions are influenced by numerous genetic and environmental factors. Types and quality of the feed and feed additives determine the characteristics and quality of products of animal origin. In terms of quality and quantity, the desired effects may be achieved only when the feed ration fully responds to the needs of the animal. Not supplying the required components with the feed affects the composition of both, colostrum and milk, and reduces their production, which influences health condition of animals and - as a result - food safety. Milk and milk products are considered functional food not only due to high nutritional value but also due to positive impact on human health. Nowadays there are various initiatives to produce the so-called healthy food obtained through specially programmed nutrition of animals, which means enriching foodstuffs in biologically active substances. In order to obtain good physicochemical parameters in colostrum and milk of ruminants, it is vital to use high quality feeds in order to satisfy protein and energy demand in animals, and mineral and vitamin contents in the feed ration should be properly balanced. It is also important to use energy additives in dry cows. Another important issue is obtaining the rumen fermentation profile that reduces the emission of methane and determines an appropriate profile of volatile fatty acids. The manuscript is devoted to physiochemical properties of colostrum and milk of cows, goats, sheep as well as the influence of nutrition on the quality of mammary secretions of ruminants.Sastav i svojstva sekreta mliječnih žlijezda sisavaca pod utjecajem su brojnih genetskih utjecaja i utjecaja okoliša. Vrsta i kvaliteta krmiva i dodataka krmnim smjesama određuju svojstva i kvalitetu proizvoda životinjskog podrijetla. S aspekta kvalitete i kvantitete željeni učinci mogu se postići samo ukoliko omjeri sastojaka u krmivu potpuno odgovaraju potrebama životinje. Nedostatna opskrba potrebnim nutrijentima utječe na sastav i kolostruma i mlijeka, te umanjuje njihovu proizvodnju, što konačno utječe na zdravstveno stanje životinje a time i na sigurnost hrane. Mlijeko i mliječni proizvodi smatraju se funkcionalnom hranom ne samo zbog visoke nutritivne vrijednosti, već i zbog povoljnih zdravstvenih učinaka na ljudski organizam. U današnje doba postoji niz inicijativa s ciljem proizvodnje tzv. zdrave hrane, primjenom posebno osmišljene hranidbe životinja, što prije svega podrazumijeva obogaćivanje hrane biološki aktivnim sastojcima. Kako bi se dobila dobra fizikalno-kemijska svojstva kolostruma i mlijeka preživača, nužno je koristiti visokokvalitetna krmiva kojima se zadovoljavaju potrebe životinje za proteinima i energijom, dok omjeri mineralnih tvari i vitamina moraju biti uravnoteženi. Također je važno koristiti dodatke za povećanje energetskog unosa u mliječnih krava. Osim toga, važno je postići mikrofloru buraga koja će omogućiti snižavanje emisije metana, kao i postizanje odgovarajućeg sastava masnih kiselina u mlijeku. Ovaj rad bavi se fizikalno-kemijskim svojstvima kravljeg, kozjeg i ovčjeg kolostruma i mlijeka, kao i utjecajem hranidbe na kvalitetu sekreta mliječnih žlijezda preživača

Influence of nutrition on the quality of colostrum and milk of ruminants

The composition and properties of mammary glands’ secretions are influenced by numerous genetic and environmental factors. Types and quality of the feed and feed additives determine the characteristics and quality of products of animal origin. In terms of quality and quantity, the desired effects may be achieved only when the feed ration fully responds to the needs of the animal. Not supplying the required components with the feed affects the composition of both, colostrum and milk, and reduces their production, which influences health condition of animals and - as a result - food safety. Milk and milk products are considered functional food not only due to high nutritional value but also due to positive impact on human health. Nowadays there are various initiatives to produce the so-called healthy food obtained through specially programmed nutrition of animals, which means enriching foodstuffs in biologically active substances. In order to obtain good physicochemical parameters in colostrum and milk of ruminants, it is vital to use high quality feeds in order to satisfy protein and energy demand in animals, and mineral and vitamin contents in the feed ration should be properly balanced. It is also important to use energy additives in dry cows. Another important issue is obtaining the rumen fermentation profile that reduces the emission of methane and determines an appropriate profile of volatile fatty acids. The manuscript is devoted to physiochemical properties of colostrum and milk of cows, goats, sheep as well as the influence of nutrition on the quality of mammary secretions of ruminants

Effects of Single Nucleotide Polymorphisms in the SLC27A3 Gene on the Nutritional Value of Sheep Milk

The current research was undertaken to use the genetic potential of animals to obtain high-quality dairy products. Single nucleotide polymorphisms (SNPs) in SLC27A3 gene were identified in Zošľachtená valaška sheep using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Correlations between genotypes and milk composition and nutritional value were analysed This study showed that milk from sheep with TT genotype in the SNP4 locus was characterised by higher (p < 0.01) fat and dry matter content and lower lactose concentration, compared to sheep with AA and TA genotypes, respectively. Moreover, it was found that animals with GG genotype in SNP1 produced milk with higher C18:1n9c, C18:1n7t, CLA, and other unsaturated fatty acids (UFAs) content than sheep with TT. Additionally, milk from animals with CC at the SNP3 locus had significantly higher (p < 0.01) levels of UFAs than milk from sheep with other genotypes in the SNP3. In summary, it may be concluded that milk from animals with TT genotype of SNP4 is characterised by higher fat and dry matter content. Whereas, milk from sheep with GG in SNP1 and with CC in SNP3 is characterised by higher content of UFAs, which increases milk value as material for functional food production

Effects of additional colostrum feeding on the levels of protein fractions in calves’ serum

The aim of this study was to investigate the impact of additional colostrum feeding to new born calves on levels of selected serum protein fractions. The study was conducted on a commercial dairy farm. Twenty-four cows of Polish Holstein-Friesian breed and their calves were included in the trial. Chemical composition, the somatic cell count (SCC), the total bacteria count (TBC) and immunoglobulin G (IgG) concentration were determined in colostrum samples. Blood was collected via jugular vein puncture from a total of 24 calves at the 36th hour of life. Total serum protein (TSP), as well as its fractions (albumins, globulins) and IgG concentration were determined. Greater IgG, total protein, albumins and globulins concentrations were found in serum of calves fed colostrum four times compared with three times on the first day of life. It was concluded that increasing the number of colostrum feedings to four times can be beneficial to forming of passive immunity of newborn calves

The Effect of Saponite Clay on Ruminal Fermentation Parameters during In Vitro Studies

Reducing the emission of global warming gases currently remains one of the strategic tasks. Therefore, the objective of our work was to determine the effect of saponite clay on fermentation in the rumen of cows. The pH, total gas production, CH4, and volatile fatty acid (VFA) production in ruminal fluid was determined in vitro. Saponite clay from the Tashkiv deposit (Ukraine) has a high content of silicon, iron, aluminum, and magnesium. The addition of 0.15 and 0.25 g of saponite clay to the incubated mixture did not change the pH but reduced the total production (19% and 31%, respectively) and CH4 (24% and 46%, respectively) in the ruminal fluid compared to the control group and had no significant effect on the total VFA levels, but propionic acid increased by 15% and 21% and butyric acid decreased by 39% and 32%, respectively. We observed a decrease in the fermentation rates, with a simultaneous increase in the P:B ratio and an increase in the fermentation efficiency (FE) in the groups fermented with saponite clay, probably a consequence of the high efficiency in the breakdown of starch in the rumen. Therefore, further in vivo studies to determine the effective dose and effect of saponite clay on cow productivity and the reduction of gas emissions are promising and important

Changes in the In Vitro Ruminal Fermentation of Diets for Dairy Cows Based on Selected Sorghum Cultivars Compared to Maize, Rye and Grass Silage

An in vitro experiment was conducted to determine the impact of silage produced from selected varieties of sorghum on the microbial fermentation profile of cows’ ruminal fluid. To determine the main microbial fermentation products, ruminal fluid samples were obtained from Polish Holstein–Friesian cows. Serum bottles were filled with 80 mL of ruminal samples, and 1 g of one of the following substrates was added: corn silage (CS), grass silage (GS), rye silage (RS), sorghum silage (sweet) (SS1), sorghum silage (grain) (SS2) or sorghum silage (dual-purpose) (SS3). The serum bottles were flushed with CO2 and fermented for 8 and 24 h at 39 °C. After incubation, the obtained gas and rumen fluid were then analysed to determine the methane and volatile fatty acid (VFA) contents using gas chromatography. The use of sorghum silage (SS) resulted in a decrease in the total concentration VFA concentration in the ruminal fluid compared with the use of other silages, especially GS. Moreover, the ruminal fluid contained a lower molar proportion of propionic and butyric acids when SS was used compared with CS. The butyric acid proportion was higher in SS samples than in RS samples. The differences in chemical composition between sorghum varieties did not influence the rumen VFA concentration or profile. A decrease in gas production, but without effects on methanogenesis, was observed when SS was used compared with GS and CS. The analysis demonstrates the physiological processes of fermentation in the rumen, as evidenced by the products of microbial fermentation. The main advantage is that the addition of SS, irrespective of the plant variety, reduced fermentation gas production in the ruminal fluid compared with CS. The silage of the analyzed sorghum varieties may be used in the diets of dairy cows as a substitute for corn and grass silages

The effects of seed from Linum usitatissimum cultivar with increased phenylpropanoid compounds and hydrolysable tannin in a high cholesterol-fed rabbit

Abstract Background Dietary fat is considered one of the most important factors associated with blood lipid metabolism and plays a significant role in the cause and prevention of atherosclerosis that has been widely accepted as an inflammatory disease of the vascular system. The aim of the present study was to evaluate the effect of genetically modified flaxseed (W86) rich in phenylpropanoid compounds and hydrolysable tannin in high cholesterol-induced atherosclerosis rabbit models compared to parental cultivar Linola. Methods Twenty-Eight White New Zealand white rabbits aged 6 months were randomly divided into four groups, control group, high cholesterol group (10 g/kg), Linola flaxseed group (100 g/kg) and W86 flaxseed group (100 g/kg). The rabbits were fed a normal diet or a high cholesterol diet for 10 weeks. Levels of blood lipids, hematological values, total antioxidative status and superoxide dismutase activity in serum were determined. Moreover, body weight and feed intake were measured after sixth and tenth weeks. After each stage of the experiment atherogenic indexes (non-HDL-C/HDL-C, LDL-C/HDL-C, and atherogenic index of plasma) was calculated. Results The intake of a dyslipidaemic diet negatively influenced lipid profile in rabbits at the 10 weeks of feeding. W86 flaxseed significantly decreased total cholesterol, LDL-C, VLDL-C and TG serum levels in cholesterolemic rabbits compared with parental Linola after 10 weeks. Atherogenic indexes decreased over time with a significant difference between the diets and they were the best for W86 flaxseed. Similarly, the experimental addition of W86 significantly decreased atherogenic predictors such as heterophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and the mean platelet volume-to-lymphocyte ratio. In rabbits, W86 flaxseed increased the activity of superoxide dismutase and total antioxidative status compared to Linola. Conclusions Results of the presented study suggest that the addition of W86 flaxseed alleviate serum lipid changes in high cholesterolemic diet-administered rabbits. W86 flaxseed significantly reduced atherogenic indexes, as compared with the Linola and indicate that W86 flaxseed more effectively red CVD risk factors during hypercholesterolemia. Moreover, the presented result suggested that W86 flaxseed can be a part of a heart-healthy and antiatherogenic diet for the human

Changes in Fatty Acid Levels during In Vitro Ruminal Fluid Incubation with Different Proportions of Maize Distillers Dried Grains (DDGS)

This study aimed to analyse changes in the profile of long-chain fatty acids in the ruminal fluid of cows during in vitro fermentation, using different proportions of maize DDGS (distillers dried grains with solubles) as a substrate. The serum bottles were filled with 1 g of concentrate feed (C), which consisted of cereal middlings, postextraction rapeseed meal, and soybean meal. Substrates I, II, and III contained the same ingredients as substrate C, but also included DDGS at increasing proportions, while substrate IV contained only DDGS. Ruminal fluid with a buffer was then added to the bottles and incubated for 4, 8, and 24 h. After incubation, the fatty acid profile was analysed using a gas chromatograph. The use of DDGS as a substrate resulted in a decrease in SFA, and an increase in the proportion of UFA, including oleic acid (C18:1n9c) and linoleic acid (C18:2n6c). The fermentation profile with 15% and 20% DDGS in TMR proved to be the most beneficial. These findings suggest that the byproduct of bioethanol production could potentially improve the fatty acid profile in the ruminal fluid, resulting in higher-quality animal products