22 research outputs found
Development of medium components for the production of glucosyl-transferring enzyme by Aureobasidium
Fermentation of a Yeast Producing Aspergillus-niger Glucose-oxidase - Scale-up, Purification and Characterization of the Recombinant Enzyme
We have developed a fermentation process to produce up to 3 grams per liter of active, secreted glucose oxidase from a recombinant Saccharomyces cerevisiae. Real-time size-exclusion HPLC analysis is used to monitor enzyme production during fermentation, and purification to more than 95 percent is obtained using only filtration methods. The recombinant enzyme is stable to higher temperatures and a wider pH range than the native Aspergillus niger enzyme, and is free of contaminating amylase, cellulase and catalase