Acriflavine, a clinically approved drug, inhibits SARS-CoV-2 and other betacoronaviruses

The COVID-19 pandemic caused by SARS-CoV-2 has been socially and economically devastating. Despite an unprecedented research effort and available vaccines, effective therapeutics are still missing to limit severe disease and mortality. Using high-throughput screening, we identify acriflavine (ACF) as a potent papain-like protease (PLpro) inhibitor. NMR titrations and a co-crystal structure confirm that acriflavine blocks the PLpro catalytic pocket in an unexpected binding mode. We show that the drug inhibits viral replication at nanomolar concentration in cellular models, in vivo in mice and ex vivo in human airway epithelia, with broad range activity against SARS-CoV-2 and other betacoronaviruses. Considering that acriflavine is an inexpensive drug approved in some countries, it may be immediately tested in clinical trials and play an important role during the current pandemic and future outbreaks. © 2021 The Author

Experimental Analysis of Control Methods in Solar Water Heating Systems

This paper focuses on an analysis of selected control methods in solar heating systems. Proportional, ON-OFF, and new proposed IPC control methods were tested. Experimental tests were conducted under natural conditions using the author’s method of clustering measurement days. In the form of thermal energy gains in the storage tanks, the results for all tested control methods are presented. The ON-OFF control method is suitable for low variability of solar radiation conditions but is ineffective under dynamic solar radiation conditions. The proportional controllers collect thermal energy under high variability solar radiation effectively, but they tend to cause thermal drifts from the system under high heat load. The proposed IPC control method prevents the waste of heat energy and makes more efficient use of the high and dynamic solar radiation. In conclusion, energy gains depend more on the level of solar radiation and less on its variability. However, the variability of solar radiation makes control in solar systems more challenging, and it is one of the factors that should determine the control strategy. The novelty of this work is based on an extension of the control algorithm by adding the temperature at the entry and exit of the solar coil. This makes it possible to eliminate thermal drift and observe the intensity of heat transfer to the water in the tank

Experimental Analysis of Control Methods in Solar Water Heating Systems

This paper focuses on an analysis of selected control methods in solar heating systems. Proportional, ON-OFF, and new proposed IPC control methods were tested. Experimental tests were conducted under natural conditions using the author’s method of clustering measurement days. In the form of thermal energy gains in the storage tanks, the results for all tested control methods are presented. The ON-OFF control method is suitable for low variability of solar radiation conditions but is ineffective under dynamic solar radiation conditions. The proportional controllers collect thermal energy under high variability solar radiation effectively, but they tend to cause thermal drifts from the system under high heat load. The proposed IPC control method prevents the waste of heat energy and makes more efficient use of the high and dynamic solar radiation. In conclusion, energy gains depend more on the level of solar radiation and less on its variability. However, the variability of solar radiation makes control in solar systems more challenging, and it is one of the factors that should determine the control strategy. The novelty of this work is based on an extension of the control algorithm by adding the temperature at the entry and exit of the solar coil. This makes it possible to eliminate thermal drift and observe the intensity of heat transfer to the water in the tank