The adipocyte component of bone marrow in heterotopic bone induced by demineralized incisor grafts

The relative proportion of adipocytes to hematopoietic elements in the marrow of heterotopicallyinduced bone evaluated 4–42 weeks post implantation of demineralized murine incisors was estimated by histologicalanalysis of hematoxylin-eosin stained tissue sections. Using computerized image analysis of microphotographs,the proportion of nuclear cells vs. adipocytes was ascertained. The percentage of adipocytes in marrowincreases over time. Such an effect, the replacement of myelopoietic marrow by adipogenic (yellow) marrowand the resorption of induced bone, is observed in human osteoporosis. A decline in the non-adipogenic cellcompartments of bone marrow accompanying induced bone begins in the fourth week of induction, graduallyprogresses until the 26th week, and does not change after that. The luminosity, a parameter used in image analysisand proportional to the number of nuclear cells, was 124 ± 3 in hematopoietic femoral bone marrow, andthat of bone marrow of the induced bone was of a similar value (117 ± 8) in the fourth week. An evident declinein luminosity of bone marrow filling the foci of heterotopic bone was observed in samples taken at nine weeks(82 ± 20). This process progressed until the 26th week, reaching a luminosity of 70 ± 21. At the 42nd week, theluminosity remained at the same level (71 ± 27). This indicates that the replacement of hematopoietic bonemarrow of heterotopically induced bone by unilocular adipocytes begins relatively early (the fourth week) and ispersistent.The relative proportion of adipocytes to hematopoietic elements in the marrow of heterotopicallyinduced bone evaluated 4–42 weeks post implantation of demineralized murine incisors was estimated by histologicalanalysis of hematoxylin-eosin stained tissue sections. Using computerized image analysis of microphotographs,the proportion of nuclear cells vs. adipocytes was ascertained. The percentage of adipocytes in marrowincreases over time. Such an effect, the replacement of myelopoietic marrow by adipogenic (yellow) marrowand the resorption of induced bone, is observed in human osteoporosis. A decline in the non-adipogenic cellcompartments of bone marrow accompanying induced bone begins in the fourth week of induction, graduallyprogresses until the 26th week, and does not change after that. The luminosity, a parameter used in image analysisand proportional to the number of nuclear cells, was 124 ± 3 in hematopoietic femoral bone marrow, andthat of bone marrow of the induced bone was of a similar value (117 ± 8) in the fourth week. An evident declinein luminosity of bone marrow filling the foci of heterotopic bone was observed in samples taken at nine weeks(82 ± 20). This process progressed until the 26th week, reaching a luminosity of 70 ± 21. At the 42nd week, theluminosity remained at the same level (71 ± 27). This indicates that the replacement of hematopoietic bonemarrow of heterotopically induced bone by unilocular adipocytes begins relatively early (the fourth week) and ispersistent

Fluvastatin increases tyrosinase synthesis induced by UVB irradiation of B16F10 melanoma cells.

Statins are widely used to lower plasma concentrations of lipids, e.g. cholesterol. One of the main effects of statin treatment is inhibition of hydroxymethyl glutaryl-coenzyme A reductase. The role of fluvastatin, a frequently used statin, was examined in potential modulation of tyrosinase (key enzyme of melanogenesis) synthesis. Levels of tyrosinase mRNA induced by UVB irradiation of B16F10 melanoma cell line were measured by real time PCR. Fluvastatin increases tyrosinase mRNA production induced by UVB irradiation in B16F10 melanoma cell line. Fluvastatin treatment may potentially influence melanin synthesis and protection against UV irradiation

Influence of unevenness of airport pavements on technical condition and safety of air operations

Abstract: The publication presents the requirements for airport pavements for the assessment of the evenness of airport pavements, including the methodology of testing unevenness. On the basis of the selected aircraft type, the impact of unevenness on the dynamic interaction of the plane - pavement was determined, and the vertical displacements occurring at the edges of concrete slabs were taken for analysis. The paper presents the influence of operational procedures in the form of pavement grooving and local surface repairs on the results of unevenness measurements and improvement of the technical condition of the pavement, as well as on the safety of air operations. Keywords: Pavement unevenness; Evaluation criteria; Dynamic action plane - surface; Grooving pavement; Maintenance treatments; The functional element of the airpor

Pituitary tumours — a large retrospective single-centre study of over 2300 cases. Experience of a tertiary reference centre

Introduction: Pituitary adenomas (PAs), also known as a pituitary neuroendocrine tumours (PitNET), are usually benign tumours of the anterior lobe of the pituitary gland and account for the third most common intracranial neoplasm. The most common type of pituitary adenoma is lactotroph adenoma, in which dopamine agonists are the first-line treatment. Nevertheless, in selected cases surgery or even radiotherapy may be required. In the current study, we aimed to analyse all patients who underwent surgery due to intrasellar mass in order to evaluate frequency of particular pituitary tumours, clinical diagnosis, and pathology findings. Material and methods: We retrospectively analysed all cases of patients consecutively operated due to intrasellar mass between 1st January 2010 and 31st December 2018 at the Department of Neurosurgery, Military Institute of Medicine in Warsaw, Poland. Results: Our database included 2348 cases: 1390 women (59.2%) and 958 men (40.8%). The mean age for women was 48.4 years (SD ± 15.72; median 49) and for men 50.9 years (SD ± 14.94; median 53). In our cohort we found: 869 gonadotroph and null cell adenomas, 751 somatotroph and mammosomatotroph adenomas, 386 corticotroph adenomas, 71 plurihormonal adenomas, 59 craniopharyngiomas, 44 lactotroph adenomas, 18 purely thyrotroph adenomas, and other rare cases of pituitary tumours including one pituitary carcinoma metastasising to the liver (corticotroph origin). Conclusions: We provide a comprehensive analysis of both clinical and pathological findings of the largest cohort of patients operated on for pituitary adenomas in one tertiary reference centre. To the best of our knowledge, this is the largest up-to-date published analysis in our country.

Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora

Abstract: HCl-demineralized murine lower incisors were implanted intramuscularly into syngeneic BALB/c mice to induce heterotopic osteogenesis. Implants were exposed at the early, preosteogenic stage (4), or at the later, osteogenic stage (12) to the Moloney sarcoma virus (MSV), which within 3–4 days results in a sarcoma. The yield of bone induction was determined by weight of dry bone mass following NaOH hydrolysis of soft tissues. To verify the effect of this sarcoma on orthotopic local femoral bone, the dry mass of the tumor-exposed femora was measured and compared with the weight of MSV-unexposed contralateral controls. MSV-sarcoma or cells involved with their spontaneous rejection have a stimulatory effect on the periosteal membrane of the tumor-adjacent femoral bones, increasing their dry mass on average by 18%. No stimulatory effect on heterotopic bone induction was observed when the MSV sarcoma grew during the early, preosteogenic stage (4 onward), but when the tooth matrix had been exposed to such tumor at the already boneforming stage, (12 onward), the yield of bone induction was enhanced. Thus, it is postulated that lesions induced by MSV during the early, preosteogenic stage inhibit recruitment of osteoprogenitor cells or degrade Bone Morphogenetic Proteins (BMPs

Tests and Assessment of the Contact Surface of Selected Measuring Tires of Friction Testers with the Airport Surface Depending on Changes in Pressure and Load

The basis of the work was to estimate the relationship between wheel pressure and wheel pressure on the surface, and the size of the contact surface. The article presents world experience in the field and presents the theoretical basis for calculating the contact area of a wheel with the road surface. The tests were carried out for two types of tires - ASTM E-1551 and ASFT T520. The tests proved a significant influence of the wheel pressure value and wheel pressure on the surface on the obtained surface area of the wheel contact with the surface