Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

The aim of the studies was to establish in vitro conditions for the culture of pea and lupin embryos as the first step in the development of an in vitro assisted single seed descent technique for the attainment of homozygous populations. Materials for the study included of pea, and narrow-leafed and yellow lupin cultivars. Embryos dissected from mature but still-green seeds were cultured in vitro on two modified MS media and under three temperature regimes. Shoot and root lengths of regenerated plants were measured after 7, 14 and 21 days of culture. For pea plants full-strength MS medium with 4 g l−1 agar and temperature 22/ 20°C (day/night) appeared to be the most conducive to shoot and root development, whereas for lupin plants lower temperatures were more propitious: 12°C in the dark for narrow-leafed lupin and 16/ 12°C (day/night) for yellow lupin. Almost all the cultured embryos developed into plants, but not all the regenerated plants survived acclimation to ex vitro conditions

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

The aim of the studies was to establish in vitro conditions for the culture of pea and lupin embryos as the first step in the development of an in vitro assisted single seed descent technique for the attainment of homozygous populations. Materials for the study included of pea, and narrow-leafed and yellow lupin cultivars. Embryos dissected from mature but still-green seeds were cultured in vitro on two modified MS media and under three temperature regimes. Shoot and root lengths of regenerated plants were measured after 7, 14 and 21 days of culture. For pea plants full-strength MS medium with 4 g l−1 agar and temperature 22/ 20°C (day/night) appeared to be the most conducive to shoot and root development, whereas for lupin plants lower temperatures were more propitious: 12°C in the dark for narrow-leafed lupin and 16/ 12°C (day/night) for yellow lupin. Almost all the cultured embryos developed into plants, but not all the regenerated plants survived acclimation to ex vitro conditions

In vitro culture of white lupin embryos as a stage for SSD technique

White lupin is an important source of proteins, the content of which amounts to 36–40% in its seeds. Breeding of new varieties usually takes several years. The present study aimed to shorten the breeding cycle by applying the single-seed descent technique together with in vitro culture of embryos dissected from immature seeds. The effects of temperature on embryo development were studied in indeterminate vs. determinate varieties in in vitro culture using the following temperature regimes:(1) 8 °C; (2) 16 °C in the first week, then 8 °C; (3) 20 °C in the first week, then 20/18 °C; and (4) 16 °C throughout the culture period. The embryos were cultured on the standard Murashige and Skoog medium. The observed growth rate of plantlets as well as the survival of plants ex vitro showed that at lower temperatures (8–16 °C) the plants developed slowly, but their ex vitro survival rate was higher compared to those grown at a higher temperature (20 °C). In addition, the results indicated that the in vitro embryo development rate of determinate varieties is significantly lower than that of indeterminate varieties