Pneumonija kod mačaka povezana sa Neisseria sp.

Neisseria sp. is a Gram-negative diplococcus bacterium usually present on the mucosal surfaces of animals without causing an obvious pathology. The objective of this study was to report the isolation of Neisseria sp. from severe cases of pyogranulomatous pneumonia with the formation of a Splendore-Hoeppli structure in two cats treated at a veterinary hospital. This paper suggests that the Neisseria genus members may be involved in lower respiratory tract infections in cats, with the molecular diagnosis being a necessary method for the correct identification of this bacteria in animals.Neisseria spp. je Gram pozitivna diplokokna bakterija koja je prisutna na mukoznim površinama životinja, pri čemu ne dovodi do uočljivih patoloških promena. Cilj ove studije bio je da opiše izolaciju Neisseria spp. kod teških slučajeva piogranulomatozne pneumonije sa formiranjem Splendore-Hoeppli strukture kod dve mačke lečene u veterinarskoj bolnici. Ovaj rad sugeriše da su članovi roda Neisseria možda uključeni u infekcije donjih disajnih puteva kod mačaka, s tim što je molekularna dijagnoza neophodan metod za tačnu identifi kaciju ove bakterije na životinjama

Determination of multidrug-resistant populations and molecular characterization of complex Klebsiella spp. in wild animals by multilocus sequence typing

Background and Aim: One of the most significant public health concerns is multidrug-resistant (MDR) microorganisms. Klebsiella spp. have been at the forefront of causing different types of infections such as bacteremia, urinary tract infections, pneumonia, enteritis, and sepsis in humans as well as animals. This study aimed to determine the genomic similarity between Klebsiella spp. isolated from wild animal samples and those described in the Institut Pasteur genomic database to verify the spread of resistant clones regionally in the state of Mato Grosso, and to compare the epidemiological data in different regions of Brazil and the world. Materials and Methods: Isolates from various sites of injury in wild animals were identified by sequencing the 16S rRNA gene. Antimicrobial susceptibility testing was performed using the disk diffusion method to verify the resistance profile, and then, multilocus sequence typing was performed to verify the population structure and compare the isolates from other regions of Brazil and the world. Results: Twenty-three sequence types (STs) were observed; of these, 11 were new STs, as new alleles were detected. There was no predominant ST among the isolates. All isolates were MDR, with high rates of resistance to sulfonamides, ampicillin, amoxicillin, and nitrofurantoin and low resistance to meropenem, imipenem, and amikacin. Conclusion: Improving our understanding of the population structure of Klebsiella spp. in wild animals may help determine the source of infection during outbreaks in humans or animals, as the One Health concept emphasizes the interlinks between humans, animals, and environmental health