The Forum: Summer 2000

Summer 2000 journal of the Honors Program at the University of North Dakota. The issue includes stories, poems, essays and art by undergraduate students.https://commons.und.edu/und-books/1043/thumbnail.jp

A Whole-Chromosome Analysis of Meiotic Recombination in Drosophila melanogaster

Although traditional genetic assays have characterized the pattern of crossing over across the genome in Drosophila melanogaster, these assays could not precisely define the location of crossovers. Even less is known about the frequency and distribution of noncrossover gene conversion events. To assess the specific number and positions of both meiotic gene conversion and crossover events, we sequenced the genomes of male progeny from females heterozygous for 93,538 X chromosomal single-nucleotide and InDel polymorphisms. From the analysis of the 30 F1 hemizygous X chromosomes, we detected 15 crossover and 5 noncrossover gene conversion events. Taking into account the nonuniform distribution of polymorphism along the chromosome arm, we estimate that most oocytes experience 1 crossover event and 1.6 gene conversion events per X chromosome pair per meiosis. An extrapolation to the entire genome would predict approximately 5 crossover events and 8.6 conversion events per meiosis. Mean gene conversion tract lengths were estimated to be 476 base pairs, yielding a per nucleotide conversion rate of 0.86 × 10−5 per meiosis. Both of these values are consistent with estimates of conversion frequency and tract length obtained from studies of rosy, the only gene for which gene conversion has been studied extensively in Drosophila. Motif-enrichment analysis revealed a GTGGAAA motif that was enriched near crossovers but not near gene conversions. The low-complexity and frequent occurrence of this motif may in part explain why, in contrast to mammalian systems, no meiotic crossover hotspots have been found in Drosophila

N-Cadherin Expression Level Distinguishes Reserved versus Primed States of Hematopoietic Stem Cells

SummaryOsteoblasts expressing the homophilic adhesion molecule N-cadherin form a hematopoietic stem cell (HSC) niche. Therefore, we examined how N-cadherin expression in HSCs relates to their function. We found that bone marrow (BM) cells highly expressing N-cadherin (N-cadherinhi) are not stem cells, being largely devoid of a Lineage−Sca1+cKit+ population and unable to reconstitute hematopoietic lineages in irradiated recipient mice. Instead, long-term HSCs form distinct populations expressing N-cadherin at intermediate (N-cadherinint) or low (N-cadherinlo) levels. The minority N-cadherinlo population can robustly reconstitute the hematopoietic system, express genes that may prime them to mobilize, and predominate among HSCs mobilized from BM to spleen. The larger N-cadherinint population performs poorly in reconstitution assays when freshly isolated but improves in response to overnight in vitro culture. Their expression profile and lower cell-cycle entry rate suggest N-cadherinint cells are being held in reserve. Thus, differential N-cadherin expression reflects functional distinctions between two HSC subpopulations

The Temperature, Electron, and Pressure Characteristics of Switchbacks: Parker Solar Probe Observations

Parker Solar Probe (PSP) observes unexpectedly prevalent switchbacks, which are rapid magnetic field reversals that last from seconds to hours, in the inner heliosphere, posing new challenges to understanding their nature, origin, and evolution. In this work, we investigate the thermal states, electron pitch angle distributions, and pressure signatures of both inside and outside switchbacks, separating a switchback into spike, transition region (TR), and quiet period (QP). Based on our analysis, we find that the proton temperature anisotropies in TRs seem to show an intermediate state between spike and QP plasmas. The proton temperatures are more enhanced in spike than in TR and QP, but the alpha temperatures and alpha-to-proton temperature ratios show the opposite trends, implying that the preferential heating mechanisms of protons and alphas are competing in different regions of switchbacks. Moreover, our results suggest that the electron integrated intensities are almost the same across the switchbacks but the electron pitch angle distributions are more isotropic inside than outside switchbacks, implying switchbacks are intact structures but strong scattering of electrons happens inside switchbacks. In addition, the examination of pressures reveals that the total pressures are comparable through a switchback, confirming switchbacks are pressure-balanced structures. These characteristics could further our understanding of ion heating, electron scattering, and the structure of switchbacks.Comment: submitted to Ap

Retired A Stars and Their Companions: Eighteen New Jovian Planets

We report the detection of eighteen Jovian planets discovered as part of our Doppler survey of subgiant stars at Keck Observatory, with follow-up Doppler and photometric observations made at McDonald and Fairborn Observatories, respectively. The host stars have masses 0.927 < Mstar /Msun < 1.95, radii 2.5 < Rstar/Rsun < 8.7, and metallicities -0.46 < [Fe/H] < +0.30. The planets have minimum masses 0.9 MJup 0.76 AU. These detections represent a 50% increase in the number of planets known to orbit stars more massive than 1.5 Msun and provide valuable additional information about the properties of planets around stars more massive thantheSun.Comment: ApJS accepted. The \rotate command prevented proper compilation. As a result Tables 19 and 21 do not fit onto the page, causing the final columns (S_HK, Nobs, respectively) to be omitte

Cellular Growth Kinetics Distinguish a Cyclophilin Inhibitor from an HSP90 Inhibitor as a Selective Inhibitor of Hepatitis C Virus

During antiviral drug discovery, it is critical to distinguish molecules that selectively interrupt viral replication from those that reduce virus replication by adversely affecting host cell viability. In this report we investigate the selectivity of inhibitors of the host chaperone proteins cyclophilin A (CypA) and heat-shock protein 90 (HSP90) which have each been reported to inhibit replication of hepatitis C virus (HCV). By comparing the toxicity of the HSP90 inhibitor, 17-(Allylamino)-17-demethoxygeldanamycin (17-AAG) to two known cytostatic compounds, colchicine and gemcitabine, we provide evidence that 17-AAG exerts its antiviral effects indirectly through slowing cell growth. In contrast, a cyclophilin inhibitor, cyclosporin A (CsA), exhibited selective antiviral activity without slowing cell proliferation. Furthermore, we observed that 17-AAG had little antiviral effect in a non-dividing cell-culture model of HCV replication, while CsA reduced HCV titer by more than two orders of magnitude in the same model. The assays we describe here are useful for discriminating selective antivirals from compounds that indirectly affect virus replication by reducing host cell viability or slowing cell growth