Liver (A); lungs (B); kidneys (C) and spleen (D) weight of untreated normal mice and B16F10 melanoma-bearing mice after DTIC, DM-1 or DTIC+DM-1 treatment.

<p>The values are expressed as mean ± s.d. Significance is indicated by #p<0.05 compared to untreated normal mice.</p

Molecular structure of DM-1.

<p>Molecular structure of DM-1.</p

Involvement of apoptotic members in melanoma tissues samples of B16F10 melanoma-bearing mice.

<p>Protein total extracts were used to analyzed Bcl-2, Bax, caspase-8, caspase-9 and caspase-3 after DTIC, DTIC+DM-1 or DM-1 treatment at the endpoint of the experiment. α-Tubulin expression was used as a loading control.</p

Macroscopic aspect of B16F10 melanoma-bearing mice on the 7^th, 10^th and 14^th days of treatment with DTIC, DM-1 or DTIC+DM-1 in comparison to control group.

<p>The measurements were made using a caliper rule.</p

Total weight and tumor mass of B16F10 melanoma-bearing mice on the 1st day of DTIC, DM-1 or DTIC+DM-1 treatment in comparison to the control group (A); Total weight and tumor mass of B16F10 melanoma-bearing mice on the 7^th day of DTIC, DM-1 or DTIC+DM-1 treatment in comparison to the control group(B); Total weight and tumor mass of B16F10 melanoma-bearing mice on the 14^th day of DTIC, DM-1 or DTIC+DM-1 treatment in comparison to the control group (C).

<p>The values are expressed as mean ± s.d. Significance is indicated by *p<0.05, **p<0.01 and ***p<0.001 compared to control.</p

Red blood cells (RBC) (A); reticulocytes (B); white blood cells (WBC) (C) and platelets (D) in peripheral blood of untreated normal mice and B16F10 melanoma-bearing mice after DTIC, DM-1 or DTIC+DM-1 treatment.

<p>The peripheral blood was collected and the cells were counted on the 1st, 7th and 14th days of treatment. The values are expressed as mean ± s.d. Significance is indicated by: *p<0.05, **p<0.01 and ***p<0.001 compared to control; and #p<0.05, ##p<0.01 and ###p<0.001 compared to untreated normal mice.</p