Mechanisms of Phosphine Toxicity

Fumigation with phosphine gas is by far the most widely used treatment for the protection of stored grain against insect pests. The development of high-level resistance in insects now threatens its continued use. As there is no suitable chemical to replace phosphine, it is essential to understand the mechanisms of phosphine toxicity to increase the effectiveness of resistance management. Because phosphine is such a simple molecule (PH3), the chemistry of phosphorus is central to its toxicity. The elements above and below phosphorus in the periodic table are nitrogen (N) and arsenic (As), which also produce toxic hydrides, namely, NH3 and AsH3. The three hydrides cause related symptoms and similar changes to cellular and organismal physiology, including disruption of the sympathetic nervous system, suppressed energy metabolism and toxic changes to the redox state of the cell. We propose that these three effects are interdependent contributors to phosphine toxicity

TreeCol: a novel approach to estimating column densities in astrophysical simulations

We present TreeCol, a new and efficient tree-based scheme to calculate column densities in numerical simulations. Knowing the column density in any direction at any location in space is a prerequisite for modelling the propagation of radiation through the computational domain. TreeCol therefore forms the basis for a fast, approximate method for modelling the attenuation of radiation within large numerical simulations. It constructs a HEALPix sphere at any desired location and accumulates the column density by walking the tree and by adding up the contributions from all tree nodes whose line of sight contributes to the pixel under consideration. In particular when combined with widely-used tree-based gravity solvers the new scheme requires little additional computational cost. In a simulation with $N$ resolution elements, the computational cost of TreeCol scales as $N \log N$, instead of the $N^{5/3}$ scaling of most other radiative transfer schemes. TreeCol is naturally adaptable to arbitrary density distributions and is easy to implement and to parallelize. We discuss its accuracy and performance characteristics for the examples of a spherical protostellar core and for the turbulent interstellar medium. We find that the column density estimates provided by TreeCol are on average accurate to better than 10 percent. In another application, we compute the dust temperatures for solar neighborhood conditions and compare with the result of a full-fledged Monte Carlo radiation-transfer calculation. We find that both methods give very similar answers. We conclude that TreeCol provides a fast, easy to use, and sufficiently accurate method of calculating column densities that comes with little additional computational cost when combined with an existing tree-based gravity solver.Comment: 11 pages, 10 figures, submitted to MNRA

Potential for using pheromone trapping and molecular screening in phosphine resistance research: Presentation

Phosphine resistance monitoring typically involves bioassays of beetles from population samples collected from grain storage facilities. Insects are classified into susceptible or resistant phenotypes based on mortality or survival at one or more discriminating doses. Although valuable, phenotype testing has several drawbacks. First, phenotype testing needs live insects, and considerable effort is required to collect and maintain them before testing. Second, population samples may contain multiple genotypes expressing different levels of resistance that may not be distinguishable using discriminating dose bioassays. Third, collections are likely to be focussed around grain storages to maximise sampling success. Recent research shows that several key pests are actively dispersing through flight. The availability of commercial pheromone lures and recent advances in molecular screening provide an opportunity to provide information on resistance gene frequencies more broadly across the landscape. This approach is proving to be a valuable adjunct to traditional resistance testing in Australia.Phosphine resistance monitoring typically involves bioassays of beetles from population samples collected from grain storage facilities. Insects are classified into susceptible or resistant phenotypes based on mortality or survival at one or more discriminating doses. Although valuable, phenotype testing has several drawbacks. First, phenotype testing needs live insects, and considerable effort is required to collect and maintain them before testing. Second, population samples may contain multiple genotypes expressing different levels of resistance that may not be distinguishable using discriminating dose bioassays. Third, collections are likely to be focussed around grain storages to maximise sampling success. Recent research shows that several key pests are actively dispersing through flight. The availability of commercial pheromone lures and recent advances in molecular screening provide an opportunity to provide information on resistance gene frequencies more broadly across the landscape. This approach is proving to be a valuable adjunct to traditional resistance testing in Australia

Utility of biotechnology based decision making tools in postharvest grain pest management: An Australian case study

A major concern for the Australian grain industry in recent years is the constant threat of resistance to the key disinfestant phosphine in a range of stored grain pests. The need to maintain the usefulness of phosphine and to contain the development of resistance are critical to international market access for Australian grain. Strong levels of resistance have already been established in major pests including the lesser grain borer, Rhyzopertha dominica (F.), the red flour beetle, Tribolium castaneum (Herbst), and most recently in the rusty grain beetle Cryptolestes ferrugineus (Stephens). As a proactive integrated resistance management strategy, new fumigation protocols are being developed in the laboratory and verified in large-scale field trials in collaboration with industry partners. To aid this development, we have deployed advanced molecular diagnostic tools to accurately determine the strength and frequency of key phosphine resistant insect pests and their movement within a typical Australian grain value chain. For example, two major bulk storage facilities based at Brookstead and Millmerran in southeast Queensland, Australia, were selected as main nodes and several farms and feed mills located in and around these two sites at a scale of 25 to 100 km radius were selected and surveyed. We determined the type, pattern, frequency as well as the distribution of resistance alleles accurately for two major pests, R. dominica and T. castaneum. Overall, this information along with the phenotypic data, provide a basis for designing key intervention strategies in managing resistance problems in the study area.A major concern for the Australian grain industry in recent years is the constant threat of resistance to the key disinfestant phosphine in a range of stored grain pests. The need to maintain the usefulness of phosphine and to contain the development of resistance are critical to international market access for Australian grain. Strong levels of resistance have already been established in major pests including the lesser grain borer, Rhyzopertha dominica (F.), the red flour beetle, Tribolium castaneum (Herbst), and most recently in the rusty grain beetle Cryptolestes ferrugineus (Stephens). As a proactive integrated resistance management strategy, new fumigation protocols are being developed in the laboratory and verified in large-scale field trials in collaboration with industry partners. To aid this development, we have deployed advanced molecular diagnostic tools to accurately determine the strength and frequency of key phosphine resistant insect pests and their movement within a typical Australian grain value chain. For example, two major bulk storage facilities based at Brookstead and Millmerran in southeast Queensland, Australia, were selected as main nodes and several farms and feed mills located in and around these two sites at a scale of 25 to 100 km radius were selected and surveyed. We determined the type, pattern, frequency as well as the distribution of resistance alleles accurately for two major pests, R. dominica and T. castaneum. Overall, this information along with the phenotypic data, provide a basis for designing key intervention strategies in managing resistance problems in the study area

Modeling Collapse and Accretion in Turbulent Gas Clouds: Implementation and Comparison of Sink Particles in AMR and SPH

We implemented sink particles in the adaptive mesh refinement (AMR) hydrodynamics code FLASH. Sink particles are created in regions of local gravitational collapse, and their trajectories and accretion can be followed over many dynamical times. We perform a series of tests including the time integration of circular and elliptical orbits, the collapse of a Bonnor-Ebert sphere and a rotating, fragmenting cloud core. We compare the collapse of a highly unstable singular isothermal sphere to the theory by Shu (1977), and show that the sink particle accretion rate is in excellent agreement with the theoretical prediction. To model eccentric orbits and close encounters of sink particles accurately, we show that a very small timestep is often required, for which we implemented subcycling of the N-body system. We emphasize that a sole density threshold for sink particle creation is insufficient in supersonic flows, if the density threshold is below the opacity limit. In that case, the density can exceed the threshold in strong shocks that do not necessarily lead to local collapse. Additional checks for bound state, gravitational potential minimum, Jeans instability and converging flows are absolutely necessary for a meaningful creation of sink particles. We apply our new sink particle module for FLASH to the formation of a stellar cluster, and compare to a smoothed particle hydrodynamics (SPH) code with sink particles. Our comparison shows encouraging agreement of gas properties, indicated by column density distributions and radial profiles, and of sink particle formation times and positions. We find excellent agreement in the number of sink particles formed, and in their accretion and mass distributions.Comment: 30 pages, 17 figures, ApJ accepted, simulation movies available at http://www.ita.uni-heidelberg.de/~chfeder/videos.shtml?lang=e

Scanned Potential Microscopy of Edge and Bulk Currents in the Quantum Hall Regime

Using an atomic force microscope as a local voltmeter, we measure the Hall voltage profile in a 2D electron gas in the quantum Hall (QH) regime. We observe a linear profile in the bulk of the sample in the transition regions between QH plateaus and a distinctly nonlinear profile on the plateaus. In addition, localized voltage drops are observed at the sample edges in the transition regions. We interpret these results in terms of theories of edge and bulk currents in the QH regime.Comment: 4 pages, 5 figure

Spectral Evolution of Energetic Neutral Atom Emissions at the Heliospheric Poles as Measured by \u3ci\u3eIBEX\u3c/i\u3e during its First Three Yeras

The Interstellar Boundary Explorer (IBEX) mission continues to measure energetic neutral atom (ENA) emissions produced by charge exchange between solar wind (SW) protons and interstellar neutrals at the edge of our heliosphere. Using the first 3 yr of IBEX-Hi ENA measurements (2009-2011), we examined the spectral evolution of ~0.5-6 keV ENAs at the polar regions (above 60°). We found the following: (1) pixels with a characteristic ankle spectra (lower spectral index at higher energies) increase by ~5% in 2010 and ~10% in 2011 compared to 2009. (2) The averaged spectral index in 2011 is smaller than that of 2009. (3) The slope of the ENA spectrum above ~1.7 keV is more variable than the slope below ~1.7 keV. The lower spectral index at higher energies of the spectrum does not appear to be caused by an increase of the ENA production at these energies, but rather from a consistent decrease at lower energies. (4) The decrease in polar ENA fluxes does not correlate significantly with the averaged SW dynamic pressure, back-traced in time to 1 AU along the flow streamlines (originating between 10° and 30° for slow SW, and 60° and 80°for fast SW), assuming these are the respective conditions of ENA progenitors back in time. These results provide insights into the complexity of relating the slow and fast SW contributions to polar ENAs and shed light on how the solar output and the resulting change in the global heliospheric structure possibly affect the heliohealth (HS) populations

An ammonia spectral map of the L1495-B218 filaments in the Taurus molecular cloud. I. Physical properties of filaments and dense cores

We present deep NH3 observations of the L1495-B218 filaments in the Taurus molecular cloud covering over a 3° angular range using the K-band focal plane array on the 100 m Green Bank Telescope. The L1495-B218 filaments form an interconnected, nearby, large complex extending over 8 pc. We observed NH3 (1, 1) and (2, 2) with a spectral resolution of 0.038 km s−1 and a spatial resolution of 31''. Most of the ammonia peaks coincide with intensity peaks in dust continuum maps at 350 and 500 μm. We deduced physical properties by fitting a model to the observed spectra. We find gas kinetic temperatures of 8–15 K, velocity dispersions of 0.05–0.25 km s−1, and NH3 column densities of 5 × 1012 to 1 × 1014 cm−2. The CSAR algorithm, which is a hybrid of seeded-watershed and binary dendrogram algorithms, identifies a total of 55 NH3 structures, including 39 leaves and 16 branches. The masses of the NH3 sources range from 0.05 to 9.5 ${{M}_{\odot }}$. The masses of NH3 leaves are mostly smaller than their corresponding virial mass estimated from their internal and gravitational energies, which suggests that these leaves are gravitationally unbound structures. Nine out of 39 NH3 leaves are gravitationally bound, and seven out of nine gravitationally bound NH3 leaves are associated with star formation. We also found that 12 out of 30 gravitationally unbound leaves are pressure confined. Our data suggest that a dense core may form as a pressure-confined structure, evolve to a gravitationally bound core, and undergo collapse to form a protostar

Comparative Toxicity of Fumigants and a Phosphine Synergist Using a Novel Containment Chamber for the Safe Generation of Concentrated Phosphine Gas

BACKGROUND: With the phasing out of ozone-depleting substances in accordance with the United Nations Montreal Protocol, phosphine remains as the only economically viable fumigant for widespread use. However the development of high-level resistance in several pest insects threatens the future usage of phosphine; yet research into phosphine resistance mechanisms has been limited due to the potential for human poisoning in enclosed laboratory environments. PRINCIPAL FINDINGS: Here we describe a custom-designed chamber for safely containing phosphine gas generated from aluminium phosphide tablets. In an improvement on previous generation systems, this chamber can be completely sealed to control the escape of phosphine. The device has been utilised in a screening program with C. elegans that has identified a phosphine synergist, and quantified the efficacy of a new fumigant against that of phosphine. The phosphine-induced mortality at 20°C has been determined with an LC(50) of 732 ppm. This result was contrasted with the efficacy of a potential new botanical pesticide dimethyl disulphide, which for a 24 hour exposure at 20°C is 600 times more potent than phosphine (LC(50) 1.24 ppm). We also found that co-administration of the glutathione depletor diethyl maleate (DEM) with a sublethal dose of phosphine (70 ppm, <LC(5)), results in a doubling of mortality in C. elegans relative to DEM alone. CONCLUSIONS: The prohibitive danger associated with the generation, containment, and use of phosphine in a laboratory environment has now been substantially reduced by the implementation of our novel gas generation chamber. We have also identified a novel phosphine synergist, the glutathione depletor DEM, suggesting an effective pathway to be targeted in future synergist research; as well as quantifying the efficacy of a potential alternative to phosphine, dimethyl disulphide