Lipid, detergent, and coomassie blue G-250 affect the migration of small membrane proteins in blue native gels:Mitochondrial carriers migrate as monomers not dimers

Background: Mitochondrial carriers were thought to be dimeric based on their migration in blue native gels.  Results: The high molecular mass species observed in blue native gels are composed of protein monomers, detergent, lipid, and Coomassie stain.  Conclusion: The mitochondrial carriers are monomeric not dimeric.  Significance: The apparent mass of small membrane proteins in blue native gels requires significant correction

Selecting hybrid pine clones for deployment - The pointy end of wood quality improvement

A clonal forestry research programme on Pinus elliottii Engelm. (slash pine) x P. caribaea Morelet var. hondurensis Barrett & Golfari (Caribbean pine) hybrids commenced in Queensland in 1986. Each cycle of clonal tests covered about 5 calendar years from field planting, and studies of wood quality variation have so far been used in selecting superior clones from the first three series of tests for commercial plantation deployment. Experience from the Series III clonal selection round is used to highlight the difficulties of ranking elite clones given a large number of growth, form, and wood property traits. Three to six ramets were felled from the best 32 clones in the Series III trials at age 6.8 years and a 3-m butt log from each was sawn into 70 × 35-mm structural boards. The clones sawn were ranked for routine deployment using data on growth, form, and wood traits. All recovered boards were assessed for distortion and tested for modulus of elasticity and modulus of rupture. Various non-destructive wood evaluation methods were used to estimate modulus of elasticity (wood stiffness) in these trees. Standing tree acoustic velocity assessed with an ST300 tool was slightly less strongly correlated phenotypically with the average modulus of elasticity of the recovered boards (r = 0.88**) than with predictions of modulus of elasticity from resonance vibration test samples and SilviScan estimates (both r = 0.89**). Moderate phenotypic relationships were found for individual tree means between average twist of the sawn boards and the average spiral grain angle of growth rings 2, 3, and 4 (r = 0.70**) assessed using a breast-height 12-mm increment core, and between average bow in the boards and average microfibril angle (r = 0.64**) from SilviScan assessments of core samples

Planetary Nebulae Kinematics in M31

We present kinematics of 135 planetary nebulae in M31 from a survey covering 3.9 square degrees and extending out to 15 kpc from the southwest major axis and more than 20 kpc along the minor axis. The majority of our sample, even well outside the disk, shows significant rotational support (mean line-of-sight velocity 116 km/s). We argue that these PN belong to the outer part of M31's large de Vaucouleurs bulge. Only five PN have velocities clearly inconsistent with this fast rotating bulge. All five may belong to tidal streams in M31's outer halo. One is projected on the Northern Spur, and is counter-rotating with respect to the disk there. Two are projected along the major axis at X=-10 kpc and have M32-like velocities; they could be debris from that galaxy. The remaining two halo PN are located near the center of the galaxy and their velocities follow the gradient found by Ibata et al. (2004), implying that these PN could belong to the Southern Stream. If M31 has a non-rotating, pressure-supported halo, we have yet to find it, and it must be a very minor component of the galaxy.Comment: accepted to ApJ; main body of paper is 36 pages, including 14 figure

Deep CCD Surface Photometry of Galaxy Clusters I: Methods and Initial Studies of Intracluster Starlight

We report the initial results of a deep imaging survey of galaxy clusters. The primary goals of this survey are to quantify the amount of intracluster light as a function of cluster properties, and to quantify the frequency of tidal debris. We outline the techniques needed to perform such a survey, and we report findings for the first two galaxy clusters in the survey: Abell 1413, and MKW 7 . These clusters vary greatly in richness and structure. We show that our surface photometry reliably reaches to a surface brightness of \mu_v = 26.5 mags per arcsec. We find that both clusters show clear excesses over a best-fitting r^{1/4} profile: this was expected for Abell 1413, but not for MKW 7. Both clusters also show evidence of tidal debris in the form of plumes and arc-like structures, but no long tidal arcs were detected. We also find that the central cD galaxy in Abell 1413 is flattened at large radii, with an ellipticity of $\approx 0.8$, the largest measured ellipticity of any cD galaxy to date.Comment: 58 pages, 24 figures, accepted for publication in the Astrophysical Journal. Version has extremely low resolution figures to comply with 650k limit. High resolution version is available at http://burro.astr.cwru.edu/johnf/icl1.ps.gz Obtaining high resolution version is strongly reccomende

Halo Star Streams in the Solar Neighborhood

We have assembled a sample of halo stars in the solar neighborhood to look for halo substructure in velocity and angular momentum space. Our sample includes red giants, RR Lyrae, and red horizontal branch stars within 2.5 kpc of the Sun with [Fe/H] less than -1.0. It was chosen to include stars with accurate distances, space velocities, and metallicities as well as well-quantified errors. We confirm the existence of the streams found by Helmi and coworkers, which we refer to as the H99 streams. These streams have a double-peaked velocity distribution in the z direction. We use the results of modeling of the H99 streams by Helmi and collaborators to test how one might use v_z velocity information and radial velocity information to detect kinematic substructure in the halo. We find that detecting the H99 streams with radial velocities alone would require a large sample. We use the velocity distribution of the H99 streams to estimate their age. From our model of the progenitor of the H99 streams, we determine that it was accreted between 6 and 9 Gyr ago. The H99 streams have [alpha/Fe] abundances similar to other halo stars in the solar neighborhood, suggesting that the gas that formed these stars were enriched mostly by Type II SNe. We have also discovered in angular momentum space two other possible substructures, which we refer to as the retrograde and prograde outliers. The retrograde outliers are likely to be halo substructure, but the prograde outliers are most likely part of the smooth halo. The retrograde outliers have significant structure in the v_phi direction and show a range of [alpha/Fe]. The methods presented in this paper can be used to exploit the kinematic information present in future large databases like RAVE, SDSSII/SEGUE, and Gaia.Comment: 46 pages, 13 figures, and 9 tables. Minor changes to text to match proofed version of the paper. Low resolution figures. High resolution version at http://www.astro.wisc.edu/~kepley/solar_streams.p

Mapping the Galactic Halo I. The `Spaghetti' Survey

We describe a major survey of the Milky Way halo designed to test for kinematic substructure caused by destruction of accreted satellites. We use the Washington photometric system to identify halo stars efficiently for spectroscopic followup. Tracers include halo giants (detectable out to more than 100 kpc), blue horizontal branch stars, halo stars near the main sequence turnoff, and the ``blue metal-poor stars'' of Preston et al (1994). We demonstrate the success of our survey by showing spectra of stars we have identified in all these categories, including giants as distant as 75 kpc. We discuss the problem of identifying the most distant halo giants. In particular, extremely metal-poor halo K dwarfs are present in approximately equal numbers to the distant giants for V fainter than 18, and we show that our method will distinguish reliably between these two groups of metal-poor stars. We plan to survey 100 square degrees at high galactic latitude, and expect to increase the numbers of known halo giants, BHB stars and turnoff stars by more than an order of magnitude. In addition to the strong test that this large sample will provide for the question `was the Milky Way halo accreted from satellite galaxies?', we will improve the accuracy of mass measurements of the Milky Way beyond 50 kpc via the kinematics of the many distant giants and BHB stars we will find. We show that one of our first datasets constrains the halo density law over galactocentric radii of 5-20 kpc and z heights of 2-15 kpc. The data support a flattened power-law halo with b/a of 0.6 and exponent -3.0. More complex models with a varying axial ratio may be needed with a larger dataset.Comment: 55 pages, 22 figures, to appear in the Astronomical Journa

Deep CCD Surface Photometry of the Edge-On Spiral NGC 4244

We have obtained deep surface photometry of the edge-on spiral galaxy NGC 4244. Our data reliably reach 27.5 R magnitude arcsec^{-2}, a significant improvement on our earlier deep CCD surface photometry of other galaxies. NGC 4244 is a nearby Scd galaxy whose total luminosity is approximately one magnitude fainter than the peak of the Sc luminosity function. We find that it has a simple structure: a single exponential disk, with a scale height h_Z = 246 +/- 2 pc, a scale length h_R = 1.84 +/- 0.02 kpc and a disk cutoff at a radius R(max) = 10.0 kpc (5.4 scale lengths). We confirm a strong cutoff in the stellar disk at R(max), which happens over only 1 kpc. We do not see any statistically significant evidence for disk flaring with radius. Unlike the more luminous Sc galaxies NGC 5907 and M 33, NGC 4244 does not show any evidence for a second component, such as a thick disk or halo, at mu(R) < 27.5 magnitude arcsec^{-2}.Comment: 36 pages, including 12 figures; accepted for publication in Sept 99 A

The HST Key Project on the Extragalactic Distance Scale. XV. A Cepheid Distance to the Fornax Cluster and Its Implications

Using the Hubble Space Telescope (HST) 37 long-period Cepheid variables have been discovered in the Fornax Cluster spiral galaxy NGC 1365. The resulting V and I period-luminosity relations yield a true distance modulus of 31.35 +/- 0.07 mag, which corresponds to a distance of 18.6 +/- 0.6 Mpc. This measurement provides several routes for estimating the Hubble Constant. (1) Assuming this distance for the Fornax Cluster as a whole yields a local Hubble Constant of 70 +/-18_{random} [+/-7]_{systematic} km/s/Mpc. (2) Nine Cepheid-based distances to groups of galaxies out to and including the Fornax and Virgo clusters yield Ho = 73 (+/-16)_r [+/-7]_s km/s/Mpc. (3) Recalibrating the I-band Tully-Fisher relation using NGC 1365 and six nearby spiral galaxies, and applying it to 15 galaxy clusters out to 100 Mpc gives Ho = 76 (+/-3)_r [+/-8]_s km/s/Mpc. (4) Using a broad-based set of differential cluster distance moduli ranging from Fornax to Abell 2147 gives Ho = 72 (+/-)_r [+/-6]_s km/s/Mpc. And finally, (5) Assuming the NGC 1365 distance for the two additional Type Ia supernovae in Fornax and adding them to the SnIa calibration (correcting for light curve shape) gives Ho = 67 (+/-6)_r [+/-7]_s km/s/Mpc out to a distance in excess of 500 Mpc. All five of these Ho determinations agree to within their statistical errors. The resulting estimate of the Hubble Constant combining all these determinations is Ho = 72 (+/-5)_r [+/-12]_s km/s/Mpc.Comment: Accepted for publication in the Astrophysical Journal, Apr. 10 issue 28 pages, 3 tables, 12 figures (Correct figures and abstract

Testing the Asteroseismic Mass Scale Using Metal-Poor Stars Characterized with APOGEE and Kepler

Fundamental stellar properties, such as mass, radius, and age, can be inferred using asteroseismology. Cool stars with convective envelopes have turbulent motions that can stochastically drive and damp pulsations. The properties of the oscillation frequency power spectrum can be tied to mass and radius through solar-scaled asteroseismic relations. Stellar properties derived using these scaling relations need verification over a range of metallicities. Because the age and mass of halo stars are well-constrained by astrophysical priors, they provide an independent, empirical check on asteroseismic mass estimates in the low-metallicity regime. We identify nine metal-poor red giants (including six stars that are kinematically associated with the halo) from a sample observed by both the Kepler space telescope and the Sloan Digital Sky Survey-III APOGEE spectroscopic survey. We compare masses inferred using asteroseismology to those expected for halo and thick-disk stars. Although our sample is small, standard scaling relations, combined with asteroseismic parameters from the APOKASC Catalog, produce masses that are systematically higher (=0.17+/-0.05 Msun) than astrophysical expectations. The magnitude of the mass discrepancy is reduced by known theoretical corrections to the measured large frequency separation scaling relationship. Using alternative methods for measuring asteroseismic parameters induces systematic shifts at the 0.04 Msun level. We also compare published asteroseismic analyses with scaling relationship masses to examine the impact of using the frequency of maximum power as a constraint. Upcoming APOKASC observations will provide a larger sample of ~100 metal-poor stars, important for detailed asteroseismic characterization of Galactic stellar populations.Comment: 4 figures; 1 table. Accepted to ApJ