PERFORMANCE OF CHROM AGAR MEDIUM AND CONVENTIONAL METHODS FOR DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS.

Background:- Methicillin resistant Staphylococcus aureus (MRSA) are responsible for hospital and community acquired infections. There are many laboratory methods for detection of MRSA. Chromogenic media have been used for the last few years for the quick detection of MRSA. Objective:- Aim of this study was to compare the performance of   conventional methods and chromogenic media for the detection of MRSA in a tertiary care hospital. Material and method: - 200 consecutive isolates of S. aureus confirmed by conventional methods, collected in a tertiary care hospital were used for this study. Cefoxitin and oxacillin disc diffusion test used as conventional methods and Chromogenic media i.e. oxacillin resistant screen agar base (ORSAB) was used for detection of methicillin resistant Staphylococcus aureus. All confirmed MRSA were checked by gold standard mecA base PCR method. Result: - Out of 200 isolates of Staphylococcus aureus, 50,52 and 47 strains were MRSA by Cefoxitin disc diffusion method, oxacillin disc diffusion method and oxacillin resistant screen agar base (ORSAB)  method respectively. Specificity was 100%, 98.66%, 98.66% by Cefoxitin disc diffusion, oxacillin disc diffusion and ORSAB method respectively. Conclusion: - In conclusion, cefoxitin disc diffusion was the best for the phenotypic detection of MRSA because their sensitivity and specificity were better than oxacillin and ORSAB

ASSOCIATION OF VIRULENCE FACTOR (PANTON–VALENTINE LEUKOCIDIN) WITH MECA GENE IN STAPHYLOCOCCUS AUREUS ISOLATES IN TERTIARY CARE HOSPITAL

 Objectives: This study was aimed to determine the association between mecA gene and virulence genes such as pvl gene in strains of S. aureus and to determine the prevalence of the pvl gene in S. aureus isolates using the polymerase chain reaction (PCR) technique.Methods: A total of 200 non-repeated, confirmed clinical isolates of S. aureus were used from various departments. Cefoxitin (30 ug) disc diffusion method was used as phenotypic method for detection of methicillin-resistant S. aureus (MRSA). We used PCR amplification to test for the pvl and mecA gene in S. aureus isolates.Results: Of 200 strains of S. aureus isolated in our hospital, 60 (30%) were identified as MRSA based on cefoxitin disc diffusion method. These same 30 isolates were confirmed for mecA gene by PCR. All strains had mecA gene. All mecA positive strains of S. aureus were tested for pvl gene. Of 200 S. aureus, 123 (61.5%) strains were pvl positive. Among which 33 (55%) were pvl positive MRSA and 90 (64.28%) pvl positive methicillin-susceptible S. aureus (MSSA) strains.Conclusion: The prevalence of the pvl among the MRSA isolates was found relatively higher in number among pus samples which indicate a possible key role of pvl in pathogenesis of pyogenic infections, especially skin and soft tissue infections in community setting

Solving Hard AI Problem using CaRP as Online Network Security

Today, there is cut throat competition in Network Security and is major issue in Computer world also several security parameters are based on hard mathematical problems are available to tackle this problem. So many researchers trying to solve this problem from last decades. Using hard AI problems for security is up-and-coming as an exciting new concept so we have to show keen interest in this domain. Hence, in this paper, we are introducing better security parameters based on hard AI problems, explicitly, a novel family of graphical password systems built on top of Captcha technology, which we are proposing Captcha and Graphical Passwords (CaRP). CaRP is both a Captcha and a graphical password system. CaRP sort some security problems, such as online guessing attacks, relay attacks and shoulder-surfing attacks. Especially, a CaRP password can be establish only probabilistically by automatic online guessing attacks even if the password is in the search position. CaRP also offers a novel approach to address the well-known image hotspot problem in popular graphical password systems, such as PassPoints that often leads to less password choices. CaRP is not a universal solution but it offers reasonable security and usability and it may use few practical applications for getting better online security such as banking, railway reservation etc. DOI: 10.17762/ijritcc2321-8169.15033

Bacteriological study of surgical site infections in a tertiary care hospital at Miraj, Maharashtra state, India

Background: Surgical site infections (SSI) are one of the common post-operative complications. Apart from bacterial contamination of wound, various patient and environment related factors play role in development and outcome of SSI. The present study is undertaken to study the frequency of SSI with reference to factors contributing to it and the antimicrobial susceptibility pattern of the causative organisms.Methods: This single-observer, cross-sectional, complete-enumeration prospective study was carried out over a period of one year. 196 pus samples from cases of surgical site infections were processed for gram staining, culture, biochemical identification tests and antimicrobial susceptibility testing. Methicillin-Resistant Staphylococcus aureus (MRSA) strains were detected by using oxacillin and cefoxitin disk diffusion and minimum inhibitory concentration (MIC) of oxacillin was tested by broth dilution technique.Results: The overall frequency of SSI was 6.17%. Most common isolates were Staphylococcus aureus, coagulase negative Staphylococci (CONS), E. coli and Pseudomonas aeruginosa. The frequency of MRSA was 8.6%. The maximum frequency was among patients operated on emergency basis in surgical department.Conclusions: The most important determinants for SSI were emergency surgery and presence of co-morbid conditions. The frequency of occurrence was age-dependent, with maximum rate of SSI in males and females in the third and sixth decades of life, respectively

Load Balancing Model in Cloud Computing Environment

As organizations need to focus on maintaining their datacenter in order to store huge amount of data of their clients. So cloud computing is one of the greatest platform which provides storage of data in very lower cost to organizations and available for all time over the internet. But it has some critical issues like load management. Load Balancing approach is based on Cloud partitioning concept. Load balancing is the process of distributing load over the different nodes which provides good resource utilization when nodes are overloaded with job. In this approach, we are using model in which memory size of every partition will be checked linearly and for efficient retrieval of user’s file, we use Bloom filter algorithm. DOI: 10.17762/ijritcc2321-8169.15036

ASSOCIATION OF VIRULENCE FACTOR (PANTON–VALENTINE LEUKOCIDIN) WITH MECA GENE IN STAPHYLOCOCCUS AUREUS ISOLATES IN TERTIARY CARE HOSPITAL

 Objectives: This study was aimed to determine the association between mecA gene and virulence genes such as pvl gene in strains of S. aureus and to determine the prevalence of the pvl gene in S. aureus isolates using the polymerase chain reaction (PCR) technique.Methods: A total of 200 non-repeated, confirmed clinical isolates of S. aureus were used from various departments. Cefoxitin (30 ug) disc diffusion method was used as phenotypic method for detection of methicillin-resistant S. aureus (MRSA). We used PCR amplification to test for the pvl and mecA gene in S. aureus isolates.Results: Of 200 strains of S. aureus isolated in our hospital, 60 (30%) were identified as MRSA based on cefoxitin disc diffusion method. These same 30 isolates were confirmed for mecA gene by PCR. All strains had mecA gene. All mecA positive strains of S. aureus were tested for pvl gene. Of 200 S. aureus, 123 (61.5%) strains were pvl positive. Among which 33 (55%) were pvl positive MRSA and 90 (64.28%) pvl positive methicillin-susceptible S. aureus (MSSA) strains.Conclusion: The prevalence of the pvl among the MRSA isolates was found relatively higher in number among pus samples which indicate a possible key role of pvl in pathogenesis of pyogenic infections, especially skin and soft tissue infections in community setting

Hospital-based study of methicillin-resistant Staphylococcus aureus in surgical site infections with special reference to determination of environmental and human sources

Background: Surgical site infection (SSI) due to methicillin-resistant Staphylococcus aureus (MRSA) is associated with increased morbidity and mortality. Carriage of MRSA by healthcare personnel is a potential source for SSI. The present study was carried out to study the frequency of MRSA in SSI; to identify the most suitable identification test for routine use; and to determine the environmental and human source of MRSA.Methods: In this prospective study, 195 SSI-pus samples were processed for primary staining, culture and biochemical tests. Cefoxitin and oxacillin disc diffusion test and oxacillin broth dilution test were used to detect MRSA and to determine the minimum inhibitory concentration (MIC) among the identified S. aureus. Environment was sampled periodically using air settle plates and swabs from various sites.  Healthcare personnel were screened for nasal and hand carriage of MRSA.Results: Of the 205 isolates, 46 were S. aureus, and among these, 18 strains were MRSA. There was no discrepency in the result by any of the three methods used. MRSA carriage, found on the hands of three healthcare personnel, had same anti-biogram as those strains simultaneously obtained from the patients. All three personnel responded to Mupirocin treatment. No MRSA was obtained from the environment.Conclusions: MRSA is an important source of SSI. Cefoxitin disc diffusion method seems suitable technique for routine use. Periodic screening of healthcare workers for carriage of MRSA will prevent outbreaks of nosocomial infections

Hospital-based study of methicillin-resistant Staphylococcus aureus in surgical site infections with special reference to determination of environmental and human sources

Background: Surgical site infection (SSI) due to methicillin-resistant Staphylococcus aureus (MRSA) is associated with increased morbidity and mortality. Carriage of MRSA by healthcare personnel is a potential source for SSI. The present study was carried out to study the frequency of MRSA in SSI; to identify the most suitable identification test for routine use; and to determine the environmental and human source of MRSA.Methods: In this prospective study, 195 SSI-pus samples were processed for primary staining, culture and biochemical tests. Cefoxitin and oxacillin disc diffusion test and oxacillin broth dilution test were used to detect MRSA and to determine the minimum inhibitory concentration (MIC) among the identified S. aureus. Environment was sampled periodically using air settle plates and swabs from various sites.  Healthcare personnel were screened for nasal and hand carriage of MRSA.Results: Of the 205 isolates, 46 were S. aureus, and among these, 18 strains were MRSA. There was no discrepency in the result by any of the three methods used. MRSA carriage, found on the hands of three healthcare personnel, had same anti-biogram as those strains simultaneously obtained from the patients. All three personnel responded to Mupirocin treatment. No MRSA was obtained from the environment.Conclusions: MRSA is an important source of SSI. Cefoxitin disc diffusion method seems suitable technique for routine use. Periodic screening of healthcare workers for carriage of MRSA will prevent outbreaks of nosocomial infections

Effect of Lead (Pb) Exposure on the Activity of Superoxide Dismutase and Catalase in Battery Manufacturing Workers (BMW) of Western Maharashtra (India) with Reference to Heme biosynthesis

The aim of this study was to estimate the activity of superoxide dismutase (SOD) and catalase in erythrocytes and malondialdehyde (MDA) in plasma of battery manufacturing workers (BMW) of Western Maharashtra (India) who were occupationally exposed to lead (Pb) over a long period of time (about 15 years). This study was also aimed to determine the Pb intoxication resulted in a disturbance of heme biosynthesis in BMW group. The blood Pb level of BMW group (n = 28) was found to be in the range of 25.8 – 78.0 μg/dL (mean + SD, 53.63 + 16.98) whereas in Pb unexposed control group (n = 35) the range was 2.8 – 22.0 μg/dL (mean + SD, 12.52 + 4.08). The blood level (Pb-B) and urinary lead level (Pb-U) were significantly increased in BMW group as compared to unexposed control. Though activated d- aminolevulinic acid dehydratase (ALAD) activities in BMW group did not show any significant change when compared to control group but activated / non activated erythrocyte – ALAD activities in BMW group showed a significant increase. Erythrocyte- zinc protoporphyrin (ZPP), urinary daminolevulinic acid (ALA-U) and porphobilinogen (PBG-U) of BMW groups elevated significantly as compared to control. A positive correlation (r = 0.66, p 1.0) were observed in control group. Hematological study revealed a significant decrease of hemoglobin concentration, packed cell volume (%) and other blood indices and a significant increase of total leucocytes count in BMW group in comparison to control group. The serum MDA content was significantly increased (p< 0.001) and the activities of antioxidant enzymes such as erythrocyte- SOD (p< 0.001) and erythrocytecatalase (p< 0.001) were significantly reduced in BMW group as compared to control group. A positive correlation (r = 0.45, p<0.02) between Pb-B and serum MDA level was observed in BMW group (Pb-B range 25.8 – 78.0 μg / dL) but such significant correlation did not notice in control group (Pb-B range 2.8 – 22.0 μg / dL).The study clearly showed an adverse effect of heme biosynthesis and imbalance of pro-oxidant / antioxidant status in lead exposed battery manufacturing workers resulting in increase in lipid peroxidation associated with decrease in erythrocyte-SOD and erythrocyte-catalase activities