Ruptured ectopic pregnancy in the rudimentary horn of bicornuate uterus: a case report

Rupture uterus in first and second trimester in a non-scarred uterus is generally seen with associated mullerian anomalies. A 22 year old primigravida with 16 weeks gestational age presented with pain in abdomen since 6 hours prior to admission and hypovolemic shock. Though she had taken regular antenatal visits and a transabdominal scan at 14-16wks with the report opining a normal study, the diagnosis of the fetus in the rudimentary horn was missed. A provisional diagnosis of rupture of right rudimentary horn of a bicornuate uterus was made afet clinical examination and the TVS showing the cervix continuing into a normal uterus with no fetus within it and a vague right adnexal mass. Transabdominal scan also showed vague hypoechoic mass in the right adnexa with a fetus of 16-17 weeks above the mass with absent cardiac activity. Emergency laparotomy with resuscitation and excision of the ruptured horn and blood transfusion saved the life of the patient.

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Bullous variant of familial biphasic lichen amyloidosis: A unique combination of three rare presentations

A 55-year-old man presented with multiple, itchy papules and macules on the trunk and extremities. Histopathologic examination of biopsy specimens taken from three different lesions showed a subepidermal blister with amyloid deposits in the dermal papillae. No systemic disease or involvement of other organs was detected. The clinical and histological findings were compatible with a bullous variant of lichen amyloidosis (LA). Primary cutaneous localized amyloidosis usually presents with papular, macular or nodular lesions. Bullous lesions associated with LA are very rare. Furthermore, patient had seven other members in the family with similar lesions, which is also a rare occurrence. We report a case with a rare combination of biphasic, bullous variant of familial LA

Primary endobronchial Hodgkin's disease

We report a case of primary pulmonary Hodgkin's disease presenting as an endobronchial mass. Tissue diagnosis was made by microscopic examination following open thoracotomy and excision biopsy of the mass. The patient responded well to the chemotherapy regimen