ADAPTIVE AND MALADAPTIVE PERSONALITY PROFILES OF ADOLESCENTS FROM DISADVANTAGED SOCIAL SETTINGS: ASSESSING GENDER AND AGE INFLUENCE

El objetivo de este estudio fue analizar los perfiles de adaptación de la personalidad en adolescentes de entornos desfavorecidos, evaluando el sexo y la edad, los efectos principales y la interacción. Se realizó un estudio transversal. Se utilizó una muestra no probabilística de 352 niños y niñas, de 13 a 18 años, de escuelas secundarias públicas. El MMPI-A se aplicó para evaluar la personalidad y también se utilizó un cronograma sociodemográfico para evaluar variables sociodemográficas y evaluar la adversidad económica. Se compararon dos grupos de perfiles de personalidad, grupos adaptativos y desadaptativos, sobre la base de los valores de T-scores de MMPI-A. Un MANOVA mostró diferencias significativas en la personalidad entre los perfiles de personalidad adaptativos y desadaptativos. Los efectos principales e interacciones por sexo y edad se encontraron en los perfiles clínicos, de contenido y complementarios. Inesperadamente, no hubo diferencias significativas por género. Se necesita más investigación para comparar los diferentes niveles de estatus socioeconómico y los entornos sociales, sin embargo, estos datos podrían proporcionar información para diseñar programas destinados a desarrollar y mejorar los rasgos de personalidad adaptativa en los adolescentes

Mechanisms by which the cystic fibrosis transmembrane conductance regulator may influence SARS-CoV-2 infection and COVID-19 disease severity

Patients with cystic fibrosis (CF) exhibit pronounced respiratory damage and were initially considered among those at highest risk for serious harm from SARS-CoV-2 infection. Numerous clinical studies have subsequently reported that individuals with CF in North America and Europe—while susceptible to severe COVID-19—are often spared from the highest levels of virus-associated mortality. To understand features that might influence COVID-19 among patients with cystic fibrosis, we studied relationships between SARS-CoV-2 and the gene responsible for CF (i.e., the cystic fibrosis transmembrane conductance regulator, CFTR). In contrast to previous reports, we found no association between CFTR carrier status (mutation heterozygosity) and more severe COVID-19 clinical outcomes. We did observe an unexpected trend toward higher mortality among control individuals compared with silent carriers of the common F508del CFTR variant—a finding that will require further study. We next performed experiments to test the influence of homozygous CFTR deficiency on viral propagation and showed that SARS-CoV-2 production in primary airway cells was not altered by the absence of functional CFTR using two independent protocols. On the contrary, experiments performed in vitro strongly indicated that virus proliferation depended on features of the mucosal fluid layer known to be disrupted by absent CFTR in patients with CF, including both low pH and increased viscosity. These results point to the acidic, viscous, and mucus-obstructed airways in patients with cystic fibrosis as unfavorable for the establishment of coronaviral infection. Our findings provide new and important information concerning relationships between the CF clinical phenotype and severity of COVID-19.This work was supported by the Italian Ministry of Health (Ministero della Salute) [Ricerca Finalizzata RF-2016-02364358]; Fondazione IRCCS Ca′ Granda Ospedale Maggiore Policlinico, Ricerca corrente; Fondazione IRCCS Ca′ Granda core COVID-19 Biobank [RC100017A]; “Liver BIBLE” [PR-0391]; and Innovative Medicines Initiative 2 joint undertaking of the European Union (EU) Horizon 2020 research and innovation programme and European Federation of Pharmaceutical Industries and Associations Programme Horizon 2020 [under grant agreement No. 777377] for the project LITMUS and the European Union, programme “Photonics” [under grant agreement 101016726] to LV. JCH was funded by the Research Council of Norway [grant no 312780] and a philanthropic donation from Vivaldi Invest A/S owned by Jon Stephenson von Tetzchner. The funders had no role in study design, data collection, data analysis, data interpretation, or writing of the report. AF was supported by a grant from the German Federal Ministry of Education and Research [01KI20197]. This work was also funded by a generous philanthropic donation from Banca Intesa San Paolo to RA. This study makes use of data generated by the GCATI Genomes for Life. Cohort study of the Genomes of Catalonia from Institut Germans Trias I Pujol (IGTP); IGTP is part of the CERCA Program/Generalitat de Catalunya. GCAT is supported by Acción de Dinamización del ISCIII-MINECO and the Ministry of Health of the Generalitat of Catalunya [ADE 10/00026], with additional support by the Agència de Gestió d'Ajuts Universitaris i de Recerca (AGAUR) [2017-SGR 529], National Grant [PI18/01512], and VEIS project [001-P-001647], co-funded by European Regional Development Fund (ERDF), “A way to build Europe.” EdW and BNW were supported by the Intramural Research Program, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH). PRT and SGS received funding from NIH NIAID [R01 AI167356].Peer reviewe

EDUCACIÓN AMBIENTAL Y SOCIEDAD. SABERES LOCALES PARA EL DESARROLLO Y LA SUSTENTABILIDAD

Este texto contribuye al análisis científico de varias áreas del conocimiento como la filosofía social, la patología, la educación para el cuidado del medio ambiente y la sustentabilidad que inciden en diversas unidades de aprendizaje de la Licenciatura en Educación para la Salud y de la Maestría en Sociología de la SaludLas comunidades indígenas de la sierra norte de Oaxaca México, habitan un territorio extenso de biodiversidad. Sin que sea una área protegida y sustentable, la propia naturaleza de la región ofrece a sus visitantes la riqueza de la vegetación caracterizada por sus especies endémicas que componen un paisaje de suma belleza

Technological development of intracellular polysilicon–chromium–gold chips for orthogonal chemical functionalization

Increasingly, advances in microtechnologies are focused on obtaining new chips intended for applications in fields such as nanomedicine and cell biology, taking advantage of the ability of microelectronics to manufacture devices with cell dimensions and a large variety of features. Here, we report a technology for the fabrication of multi-material chips, using polysilicon and gold as device layers, to be used as bi-functional cell-internalizable devices. In our case, one of the main technological challenges is to overcome the low adherence between these two materials, especially because of their small contact-area, only 9 μm2. Thus, in order to circumvent this difficulty a chromium adherent-layer was deposited in between. After fabrication, the devices following this design can be successfully internalized inside living macrophages without affecting their viability. The advantage of having multiple material layers in one device is the potential to render multi-tasking chips, as once they are appropriately functionalized, we can provide the chip the ability of being multi-functional. Hence, and as a proof of concept, two different proteins, Wheat Germ Agglutinin (WGA) and Concanavalin (ConA), were immobilized on the chip surface through self-assembled monolayers using orthogonal chemistry. The results of this work show a well-controlled fabrication, the bi-functional capabilities and no cell-toxicity of intracellular polysilicon–chromium–gold chips. Eventually, two different dyes (Oregon Green® 488 and BODIPY® 581/591) were used to bi-functionalize each surface of the multi-material chip in order to demonstrate that functional chips can also be internalized in living cells. These devices have a promising future as intracellular functional platforms for biosensing, drug delivery and diagnosis

Technological development of intracellular polysilicon-chromium-gold chips for orthogonal chemical functionalization

© 2014 Elsevier B.V. Increasingly, advances in microtechnologies are focused on obtaining new chips intended for applications in fields such as nanomedicine and cell biology, taking advantage of the ability of microelectronics to manufacture devices with cell dimensions and a large variety of features. Here, we report a technology for the fabrication of multi-material chips, using polysilicon and gold as device layers, to be used as bi-functional cell-internalizable devices. In our case, one of the main technological challenges is to overcome the low adherence between these two materials, especially because of their small contact-area, only 9 μm2. Thus, in order to circumvent this difficulty a chromium adherent-layer was deposited in between. After fabrication, the devices following this design can be successfully internalized inside living macrophages without affecting their viability. The advantage of having multiple material layers in one device is the potential to render multi-tasking chips, as once they are appropriately functionalized, we can provide the chip the ability of being multi-functional. Hence, and as a proof of concept, two different proteins, Wheat Germ Agglutinin (WGA) and Concanavalin (ConA), were immobilized on the chip surface through self-assembled monolayers using orthogonal chemistry. The results of this work show a well-controlled fabrication, the bi-functional capabilities and no cell-toxicity of intracellular polysilicon-chromium-gold chips. Eventually, two different dyes (Oregon Green® 488 and BODIPY® 581/591) were used to bi-functionalize each surface of the multi-material chip in order to demonstrate that functional chips can also be internalized in living cells. These devices have a promising future as intracellular functional platforms for biosensing, drug delivery and diagnosis.Peer Reviewe