Caste Identities and Structures of Threats: Stigma, Prejudice and Social Representation in Indian universities

Caste is a complex ontological construction. Despite several anti-caste move-ments and constitutional provisions, caste exists in the Indian psyche as part of everyday life. Even in the advent of globalization, caste continues to foster social and economic inequalities and exclusion in newer forms and perpetuates violence. The available research on caste-based stigma and humiliation provides a limited understanding as it deals with Dalits only; and ignores caste-Hindus (upper-caste) agency. Based largely on qualitative data collected at an intense three-day workshop, including two Focus Group Discussions and a year-long ethnography, this article illustrates the micro processes of everyday life experiences of caste-based stigma and humiliation among university students, academic staff and administrative staff. It explores subtle and overt caste discrimination, prejudices and stereotypes existing in the spatial morphologies of Indian higher education, its perpetuation on campuses and its impact on students’ psyche. It highlights the dearth of scholarship in this area of caste identity and stigma; and proposes nuanced questions for future research to understand why universities in India are turning into places of social defeat for Dalit and OBC students. The article argues the basis of caste discrimination and humiliation in universities is not the same as it exists in other social institutions. Instead of asserting conclusions on this matter we set out justifiable lines of inquiry. There are two issues that this article examines first, how students in Indian higher education evolve strategies for coping with threatened identities. Second, what structural repair in higher education is required to heal the wounded (caste) psyche

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Caste Identities and Structures of Threats: Stigma, Prejudice and Social Representation in Indian universities

Caste is a complex ontological construction. Despite several anti-caste movements and constitutional provisions, caste exists in the Indian psyche as part of everyday life. Even in the advent of globalization, caste continues to foster social and economic inequalities and exclusion in newer forms and perpetuates violence. The available research on caste-based stigma and humiliation provides a limited understanding as it deals with Dalits only; and ignores caste-Hindus (upper-caste) agency. Based largely on qualitative data collected at an intense three-day workshop, including two Focus Group Discussions and a year-long ethnography, this article illustrates the micro processes of everyday life experiences of caste-based stigma and humiliation among university students, academic staff and administrative staff. It explores subtle and overt caste discrimination, prejudices and stereotypes existing in the spatial morphologies of Indian higher education, its perpetuation on campuses and its impact on students’ psyche. It highlights the dearth of scholarship in this area of caste identity and stigma; and proposes nuanced questions for future research to understand why universities in India are turning into places of social defeat for Dalit and OBC students. The article argues the basis of caste discrimination and humiliation in universities is not the same as it exists in other social institutions. Instead of asserting conclusions on this matter we set out justifiable lines of inquiry. There are two issues that this article examines: first, how students in Indian higher education evolve strategies for coping with threatened identities. Second, what structural repair in higher education is required to heal the wounded (caste) psyche?</jats:p

A synthetic communication system uncovers self-jamming of bacteriophage transmission

Intercellular communication enables coordinated action by cells of microbial communities and multicellular organisms, often mediated by molecular exchange of information. Inspired by their success, synthetic biologists have recently started implementing population-level controls in engineered organisms with the aim of expanding circuit size and complexity. Yet, realising the true potential of multicellular synthetic biology requires an expanded communication alphabet as well as quantitative models to predict complex behaviour. Towards that aim, here we repurpose the M13 bacteriophage machinery for cell-to-cell communication between Escherichia coli cells and characterise the signalling dynamics. The fitted quantitative model includes the growth burden of the communication machinery, the relationship between cellular growth phase and the secretion-infection kinetics, and concurrent antibiotic selection. Limitations of deterministic models are demonstrated, with stochastic effects playing a key role in reproducing the observed infection kinetics. Surprisingly, we discover that the M13 minor coat protein pIII is released into the medium to confer extracellular immunity to uninfected cells. In a simulated gut environment, this mechanism enables the phage to farm uninfected bacterial cells for the future, increasing the overall success of both M13 and E. coli. In addition to establishing a tool for intercellular communication, our work uncovers the mutualistic nature of a phage-bacterial relationship that has evolved over long-term coexistence

The Topology of the L-Arginine Exporter ArgO Conforms to an N-in-C-out Configuration in Escherichia coli: Requirement for the Cytoplasmic N-Terminal Domain, Functional Helical Interactions, and an Aspartate Pair for ArgO Function

ArgO and LysE are members of the LysE family of exporter proteins and ordinarily mediate the export of L-arginine (Arg) in Escherichia coli and L-lysine (Lys) and Arg in Corynebacterium glutamicum, respectively. Under certain conditions, ArgO also mediates Lys export. To delineate the arrangement of ArgO in the cytoplasmic membrane of E. coli, we have employed a combination of cysteine accessibility in situ, alkaline phosphatase fusion reporters, and protein modeling to arrive at a topological model of ArgO. Our studies indicate that ArgO assumes an N-in-C-out configuration, potentially forming a five-transmembrane helix bundle flanked by a cytoplasmic N-terminal domain (NTD) comprising roughly its first 38 to 43 amino acyl residues and a short periplasmic C-terminal region (CTR). Mutagenesis studies indicate that the CTR, but not the NTD, is dispensable for ArgO function in vivo and that a pair of conserved aspartate residues, located near the opposing edges of the cytoplasmic membrane, may play a pivotal role in facilitating transmembrane Arg flux. Additional studies on amino acid substitutions that impair ArgO function in vivo and their derivatives bearing compensatory amino acid alterations indicate a role for intramolecular interactions in the Arg export mechanism, and some interactions are corroborated by normal-mode analyses. Lastly, our studies suggest that ArgO may exist as a monomer in vivo, thus highlighting the requirement for intramolecular interactions in ArgO, as opposed to interactions across multiple ArgO monomers, in the formation of an Arg-translocating conduit. IMPORTANCE The orthologous proteins LysE of C. glutamicum and ArgO of E. coli function as exporters of the basic amino acids L-arginine and L-lysine and the basic amino acid L-arginine, respectively, and LysE can functionally substitute for ArgO when expressed in E. coli. Notwithstanding this functional equivalence, studies reported here show that ArgO possesses a membrane topology that is distinct from that reported for LysE, with substantial variation in the topological arrangement of the proximal one-third portions of the two exporters. Additional genetic and in silico studies reveal the importance of (i) the cytoplasmic N-terminal domain, (ii) a pair of conserved aspartate residues, and (iii) potential intramolecular interactions in ArgO function and indicate that an Arg-translocating conduit is formed by a monomer of ArgO

Small Molecule IITR08367 Potentiates Antibacterial Efficacy of Fosfomycin against Acinetobacter baumannii by Efflux Pump Inhibition

Fosfomycin is a broad-spectrum single-dose therapy approved for treating lower urinary tract infections. Acinetobacter baumannii, one of the five major UTI-causing pathogens, is intrinsically resistant to fosfomycin. Reduced uptake and active efflux are major reasons for this intrinsic resistance. AbaF, a major facilitator superfamily class of transporter in A. baumannii, is responsible for fosfomycin efflux and biofilm formation. This study describes the identification and validation of a novel small-molecule efflux pump inhibitor that potentiates fosfomycin efficacy against A. baumannii. An AbaF inhibitor screening was performed against Escherichia coli KAM32/pUC18_abaF, using the noninhibitory concentration of 24 putative efflux pump inhibitors. The inhibitory activity of IITR08367 [bis(4-methylbenzyl) disufide] against fosfomycin/H+ antiport was validated using ethidium bromide efflux, quinacrine-based proton-sensitive fluorescence, and membrane depolarization assays. IITR08367 inhibits fosfomycin/H+ antiport activity by perturbing the transmembrane proton gradient. IITR08367 is a nontoxic molecule that potentiates fosfomycin activity against clinical strains of A. baumannii and prevents biofilm formation by inhibiting efflux pump (AbaF). The IITR08367-fosfomycin combination reduced bacterial burden by > 3 log10 in kidney and bladder tissue in the murine UTI model. Overall, fosfomycin, in combination with IITR08367, holds the potential to treat urinary tract infections caused by A. baumannii

Banded versus Single-sided bonded space maintainers: A Comparative Study

Background: The present study is conducted to evaluate and compare the clinical performance of conventional band and loop space maintainer and fiber reinforced composite resin (FRCR) space maintainers. Materials and Methods: A total of 45 extraction sites in the age group of 6–9 years having premature loss of primary molars or indicated for extraction were selected for the study. The patients were randomly divided into three groups as Group I, in which conventional band and loop space maintainer was given, Group II and Group III (FRCR), in which FRCR (everStick CandB) and impregnated glass fibers (Interlig) space maintainers were given, respectively. Patients were recalled at 3, 6, and 12-month interval for evaluation of all the three types of space maintainer. Results: Overall success rate of Group I was 86.7%, for Group II was 80%, and for Group III was 73.3% at the end of the study. Patient acceptability was significantly higher in Group II and Group III (FRCR) as compared to Group I (Conventional band and loop). In Group I, cement loss and fracture of loop, whereas in Group II and Group III, debonding at enamel composite was the most common failure followed by debonding at fiber composite and fiber fracture. FRCR space maintainers were found to be cost-effective as compared to Group I. More linear changes and angular changes were recorded in Group I as compared to Group II and Group III but difference was not significant (P > 0.05). Conclusion: Only single (buccal) surface application of FRCR space maintainers showed almost equal clinical efficacy compared to conventional band and loop space maintainer with significantly better patient acceptability, less cost, and time taken