Multiplex-PCR protocol development for rapid screening of white spot syndrome virus (WSSV) in shrimp

This study was aimed to develop a faster single step multiplex PCR protocol for the simultaneous detection of white spot syndrome virus (WSSV) with its host (i.e. shrimp) as internal positive control. To do so, four combinations of primer were tested (I. 16S rRNA+Lo F1R1; II. 16S rRNA+Lo F2R2; III. 16S rRNA+Lo F1R2; IV. 16S rRNA+Lo F2R1) which were selected based on two pairs of WSSV specific primer (Lo F1R1 and Lo F2R2) and one pair of shrimp specific primer (16S rRNA). DNA extracted from WSSV infected shrimp were amplified by PCR in a single tube using each of the primer combinations and the thermal cycling conditions as well as reagent compositions were optimized. All the primer combinations yielded their expected band sizes with stronger band resolution intensity that indicated the development of four multiplex PCR protocols. The developed multiplex protocols reduced the chance of cross contamination and these were found to be faster, single step and unique with less effort and resource use. Considering sensitivity and specificity, among the protocols, we suggested the protocols based on 16S rRNA+Lo F1R1 and/or 16S rRNA+Lo F2R2 primer combinations for rapid and routine screening of WSSV in shrimp PL, juvenile and adult

Design of an Automated White Board Profiler

This paper presents the design for an automated and modernized white board profiler. The design objectives were to: (a) Introducing the concept for a new method of white board cleaning. (b) Getting rid of time consuming old methods. (c) Introducing the design with a CAD model with circuit analysis. Proving that it is time and labor efficient while being cost efficient to do it by using electricity and mechanical body Also, enhancing the accessibility of the user and increasing efficiency in terms of use. This work is expected to contribute to the overall knowledge of machine design and white board cleaning automation in specific

Design of an Automated White Board Profiler

This paper presents the design for an automated and modernized white board profiler. The design objectives were to: (a) Introducing the concept for a new method of white board cleaning. (b) Getting rid of time consuming old methods. (c) Introducing the design with a CAD model with circuit analysis. Proving that it is time and labor efficient while being cost efficient to do it by using electricity and mechanical body Also, enhancing the accessibility of the user and increasing efficiency in terms of use. This work is expected to contribute to the overall knowledge of machine design and white board cleaning automation in specific

COVID-19 in Bangladesh: A systematic review of the literature from march 2020 to march 2021

COVID-19 has undoubtedly absorbed the global public\u27s angst. It has quickly disturbed global life and will have long-term and short-term consequences on several sectors. This comprehensive review study\u27s goal is to discover what research has been done in a year since the beginning of coronavirus in Bangladesh. Consequently, the current study examined the pervasiveness and affiliation of social and economic issues, health and psychological issues and individual perceptions, key challenges, strategies and policy systems, public health, online education, agriculture and food security, criminal activities related to outbreaks, Rohingya refugee issues, and the quality of domestic violence behaviors. The review used electronic databases such as Web of Science, PubMed, PubMed Central, and Scopus to find published material. This study reviewed chosen papers, removing redundancies and including 43 pertinent articles. Among the 43 study items, fourteen were qualitative (32.56%), six mixed (13.95%), and the rest were quantitative (53.49 %). This study helps to identify the issues with current documentation by focusing on interconnected factors and studying COVID-19 events and scenarios. Thus, governments and other stakeholders should reassess these controversial issues to formulate a policy that takes into consideration the situation in Bangladesh and other COVID-19 affected countries

COVID-19 in Bangladesh: A systematic review of the literature from march 2020 to march 2021

COVID-19 has undoubtedly absorbed the global public\u27s angst. It has quickly disturbed global life and will have long-term and short-term consequences on several sectors. This comprehensive review study\u27s goal is to discover what research has been done in a year since the beginning of coronavirus in Bangladesh. Consequently, the current study examined the pervasiveness and affiliation of social and economic issues, health and psychological issues and individual perceptions, key challenges, strategies and policy systems, public health, online education, agriculture and food security, criminal activities related to outbreaks, Rohingya refugee issues, and the quality of domestic violence behaviors. The review used electronic databases such as Web of Science, PubMed, PubMed Central, and Scopus to find published material. This study reviewed chosen papers, removing redundancies and including 43 pertinent articles. Among the 43 study items, fourteen were qualitative (32.56%), six mixed (13.95%), and the rest were quantitative (53.49 %). This study helps to identify the issues with current documentation by focusing on interconnected factors and studying COVID-19 events and scenarios. Thus, governments and other stakeholders should reassess these controversial issues to formulate a policy that takes into consideration the situation in Bangladesh and other COVID-19 affected countries

Seed Priming Influence on High Temperature Tolerance and Weed Suppressive Ability of Late Sown Dry Direct Seeded Winter Rice

Seed priming is a pre-sowing hydration technique which leads to a physiological condition triggering germination and enhancing uniform seedling emergence. It is a promising tool for enhancing drought tolerance in plants which is essential for promoting economic development by coping up with the adverse effects of climate change on crop productivity. So, a better understanding of seed priming efficacy is required. Therefore an experiment was conducted with a view to investigating the effect of seed priming agent on the weed growth and yield performance of high yielding rice variety BRRI dhan29 sown on different dates following dry direct seeded condition in boro season. Two sowing dates viz., 20th January (early or optimum sowing as control) and 20th February (late sowing as high temperature stress during reproductive stage) and seed priming agents included NaCl (20000 and 30000 ppm), KCl (20000 and 30000 ppm), CaCl2 (20000 and 30000 ppm), CuSO4 (50 and 75 ppm), ZnSO4 (10000 and 15000 ppm), Na2MoO4 (2 and 3 ppm) and PEG (100 and 150 ppm) were used. Plant height and tillers of BRRI dhan29 were significantly enhanced when seeds were sown early (20th January) and due to seed priming at early stage and at harvest. Among the yield parameters grains panicle-1, grain yield, and straw yield were produced more by early sowing (20th January). Grains panicle-1, 1000-grain weight, and grain yield were positively influenced due to seed priming. Considering the growth and yield parameters and yield of rice, early sowing and seed priming with KCl or CaCl2 were found as the best. Seed priming produced no significant effect on the weed growth. There was no significant effect of interaction between sowing date and priming agent on crop characters and yield parameters. Therefore, seed priming with 20000 ppm KCl or 20000 ppm CaCl2 and early sowing is recommended for increasing the yield of dry direct seeded boro rice (BRRI dhan29). If somehow early sowing is not possible, seed priming with 20000 ppm KCl or 20000 ppm CaCl2 is highly recommended or mitigating temperature stress during reproductive stage and enhancing yield of BRRI dhan29 under dry direct seeded, late sowncondition. [J Bangladesh Agril Univ 2022; 20(4.000): 383-392

Gender-based vulnerability and adaptive capacity in the disaster-prone coastal areas from an intersectionality perspective

Households in the coastal areas are more vulnerable to various environmental, social, and economic disruptions in terms of an intersectionality point of view. As a first step in mitigating potential effects on families, knowing how susceptible they are and, ideally, fortifying themselves against existing and potential disruptions is essential. Vulnerability and adaptive capacity could not be uniformly distributed between households owing to gender-based socio-economic disparities and inequities. This research, thereby, examined the vulnerability and adaptive capacity variation between households headed by males and females in the two coastal areas of an emerging economy like Bangladesh. This study utilized the Evaluation-based on the Distance from Average Solution (EDAS) method and Boosted Regression Trees (BRT) technique to conduct the analysis. The EDAS method has been used to analyze the adaptive capacity index. Using BRT, an innovative approach in the area, we showed that male and female-headed households are different in terms of their capability to adapt. The findings from this study suggest that the households led by females are more vulnerable than those headed by males in the study region across a variety of dimensions (social, health, economic, housing, and land ownership) from an intersectionality perspective. The study findings can provide a new outlook for the decision-makers in the coastal region on the vulnerability and adaptive capacity differences among the residents and thus lead to more efficient disaster management practices