Project and Realization of a Wide-Range High-Frequency RFID Gate Allowing Omnidirectional Detection of Transponders

The paper describes the study and development of a 2-meter-wide HF RFID gate providing omnidirectional detection of transponders.Common commercial HF RFID gate structures provide a maximum reading range around 150 cm.Moreover, this value is in most cases guaranteed only for the maximum coupling direction, with lower values for the other 2 orientations. The proposed structure raises the value of the reading range up to 200 cm for every orientation of the transponder, with even better results (220 cm) when the transponder is in the position of maximum coupling. This result has been achieved through numerical simulations, focused on the study of the geometry of the antenna system and on the realization of the matching circuit and then confirmed with the physical implementation of the system

RFID Applications for Sanitary Environments

Healthcare represents one of the most significant sectors where the diffusion of RFID technology is growing day by day. Many different applications have already been studied and developed, with both active and passive devices working at all the available operative frequencies. Sanitary environments are nowadays extremely complex structures employing several thousands of people with very strict safety requirements: in emergency situations for example 5 minutes can make the difference for a patient between survive and die. RFID is especially indicated to be employed in these scenarios for two main reasons: first of all because it’s a particularly reliable technology, with good performances, few errors and fast interaction, and secondly because, due to the presence of many different technological systems, ad-hoc solutions can be designed on the specific requirements of the application to be realized. At present the most common RFID applications in healthcare can be divided into two main categories: the items tracking and the tracking and identification of people, patients and sanitary operators. The items tracking is performed in order to avoid the loss of expensive devices and to reduce wasting of time during assistance operations: systems studied and realized for this purpose cover all the range of RFID systems and provide different services according to their different performances. RFID systems for the identification of people probably represent the most interesting sector, due to the variety of different applications that can be studied and realized. The most common systems foresee the use of RFID for the tracking of sanitary operators or patients during their assistance operations. Along with this many other applications have been implemented, including systems operating the unambiguous matching between the patient and his treatments (for example the medicine or the blood sack) or between the mother and the child in the paediatrics departments. Finally the availability of a memory on the transponders fostered to the use of RFID technology also as a mean to promptly store and retrieve patient related information: for example electronic case history or electronic medical prescription applications have been studied and developed

Discovery of the 3-Amino-1,2,4-triazine-Based Library as Selective PDK1 Inhibitors with Therapeutic Potential in Highly Aggressive Pancreatic Ductal Adenocarcinoma

Pyruvate dehydrogenase kinases (PDKs) are serine/threonine kinases, that are directly involved in altered cancer cell metabolism, resulting in cancer aggressiveness and resistance. Dichloroacetic acid (DCA) is the first PDK inhibitor that has entered phase II clinical; however, several side effects associated with weak anticancer activity and excessive drug dose (100 mg/kg) have led to its limitation in clinical application. Building upon a molecular hybridization approach, a small library of 3-amino-1,2,4-triazine derivatives has been designed, synthesized, and characterized for their PDK inhibitory activity using in silico, in vitro, and in vivo assays. Biochemical screenings showed that all synthesized compounds are potent and subtype-selective inhibitors of PDK. Accordingly, molecular modeling studies revealed that a lot of ligands can be properly placed inside the ATP-binding site of PDK1. Interestingly, 2D and 3D cell studies revealed their ability to induce cancer cell death at low micromolar doses, being extremely effective against human pancreatic KRAS mutated cancer cells. Cellular mechanistic studies confirm their ability to hamper the PDK/PDH axis, thus leading to metabolic/redox cellular impairment, and to ultimately trigger apoptotic cancer cell death. Remarkably, preliminary in vivo studies performed on a highly aggressive and metastatic Kras-mutant solid tumor model confirm the ability of the most representative compound 5i to target the PDH/PDK axis in vivo and highlighted its equal efficacy and better tolerability profile with respect to those elicited by the reference FDA approved drugs, cisplatin and gemcitabine. Collectively, the data highlights the promising anticancer potential of these novel PDK-targeting derivatives toward obtaining clinical candidates for combatting highly aggressive KRAS-mutant pancreatic ductal adenocarcinomas

Structural Manipulations of Marine Natural Products Inspire a New Library of 3-Amino-1,2,4-Triazine PDK Inhibitors Endowed with Antitumor Activity in Pancreatic Ductal Adenocarcinoma

Pancreatic ductal adenocarcinoma (PDAC) is one of the main aggressive types of cancer, characterized by late prognosis and drug resistance. Among the main factors sustaining PDAC progression, the alteration of cell metabolism has emerged to have a key role in PDAC cell proliferation, invasion, and resistance to standard chemotherapeutic agents. Taking into account all these factors and the urgency in evaluating novel options to treat PDAC, in the present work we reported the synthesis of a new series of indolyl-7-azaindolyl triazine compounds inspired by marine bis-indolyl alkaloids. We first assessed the ability of the new triazine compounds to inhibit the enzymatic activity of pyruvate dehydrogenase kinases (PDKs). The results showed that most of derivatives totally inhibit PDK1 and PDK4. Molecular docking analysis was executed to predict the possible binding mode of these derivatives using ligand-based homology modeling technique. Evaluation of the capability of new triazines to inhibit the cell growth in 2D and 3D KRAS-wild-type (BxPC-3) and KRAS-mutant (PSN-1) PDAC cell line, was carried out. The results showed the capacity of the new derivatives to reduce cell growth with a major selectivity against KRAS-mutant PDAC PSN-1 on both cell models. These data demonstrated that the new triazine derivatives target PDK1 enzymatic activity and exhibit cytotoxic effects on 2D and 3D PDAC cell models, thus encouraging further structure manipulation for analogs development against PDA

A low power IoT sensor node architecture for waste management within smart cities context

This paper focuses on the realization of an Internet of Things (IoT) architecture to optimize waste management in the context of Smart Cities. In particular, a novel typology of sensor node based on the use of low cost and low power components is described. This node is provided with a single-chip microcontroller, a sensor able to measure the filling level of trash bins using ultrasounds and a data transmission module based on the LoRa LPWAN (Low Power Wide Area Network) technology. Together with the node, a minimal network architecture was designed, based on a LoRa gateway, with the purpose of testing the IoT node performances. Especially, the paper analyzes in detail the node architecture, focusing on the energy saving technologies and policies, with the purpose of extending the batteries lifetime by reducing power consumption, through hardware and software optimization. Tests on sensor and radio module effectiveness are also presented

Outcomes of elective liver surgery worldwide: a global, prospective, multicenter, cross-sectional study

Background: The outcomes of liver surgery worldwide remain unknown. The true population-based outcomes are likely different to those vastly reported that reflect the activity of highly specialized academic centers. The aim of this study was to measure the true worldwide practice of liver surgery and associated outcomes by recruiting from centers across the globe. The geographic distribution of liver surgery activity and complexity was also evaluated to further understand variations in outcomes. Methods: LiverGroup.org was an international, prospective, multicenter, cross-sectional study following the Global Surgery Collaborative Snapshot Research approach with a 3-month prospective, consecutive patient enrollment within January–December 2019. Each patient was followed up for 90 days postoperatively. All patients undergoing liver surgery at their respective centers were eligible for study inclusion. Basic demographics, patient and operation characteristics were collected. Morbidity was recorded according to the Clavien–Dindo Classification of Surgical Complications. Country-based and hospital-based data were collected, including the Human Development Index (HDI). (NCT03768141). Results: A total of 2159 patients were included from six continents. Surgery was performed for cancer in 1785 (83%) patients. Of all patients, 912 (42%) experienced a postoperative complication of any severity, while the major complication rate was 16% (341/2159). The overall 90-day mortality rate after liver surgery was 3.8% (82/2,159). The overall failure to rescue rate was 11% (82/ 722) ranging from 5 to 35% among the higher and lower HDI groups, respectively. Conclusions: This is the first to our knowledge global surgery study specifically designed and conducted for specialized liver surgery. The authors identified failure to rescue as a significant potentially modifiable factor for mortality after liver surgery, mostly related to lower Human Development Index countries. Members of the LiverGroup.org network could now work together to develop quality improvement collaboratives

LoRaWAN for Vehicular Networking: Field Tests for Vehicle-to-Roadside Communication

Vehicular wireless networks are one of the most valuable tools for monitoring platforms in the automotive domain. At the same time, Internet of Things (IoT) solutions are playing a crucial role in the same framework, allowing users to connect to vehicles in order to gather data related to their working cycle. Such tasks can be accomplished by resorting to either cellular or non-cellular wireless technologies. While the former can ensure low latency but require high running costs, the latter can be employed in quasi-real-time applications but definitely reduce costs. To this end, this paper proposes the results of two measurement campaigns aimed at assessing the performance of the long-range wide-area network (LoRaWAN) protocol when it is exploited as an enabling technology to provide vehicles with connectivity. Performances are evaluated in terms of packet loss (PL) and received signal strength indicator (RSSI) in wireless links. The two testing scenarios consisted of a transmitter installed on a motorbike running on an elliptical track and a receiver placed in the centre of the track, and a transmitter installed on the roof of a car and a receiver placed next to a straight road. Several speeds were tested, and all the spreading factors (SFs) foreseen by the protocol were examined, showing that the Doppler effect has a marginal influence on the receiving performance of the technology, and that, on the whole, performance is not significantly affected by the speed. Such results prove the feasibility of LoRaWAN links for vehicular network purposes

LoPATraN: Low Power Asset Tracking by Means of Narrow Band IoT (NB-IoT) Technology

The narrowband Internet-of-Things (NB-IoT) communication standard is gaining momentum within the big picture of the Internet-of-Things (IoT) owing to its capabilities of ensuring pervasive and wide coverage while limiting power consumption. Therefore, it turns out to be a valuable enabling technology within a considerable number of applications. Apart from traditional remote monitoring and data acquisition purposes where comparable Low Power Wide Area Network (LPWAN) facilities have ruled for years, NB-IoT can potentially carve out space within specific alcoves in which low latency, low power, high data-rates and ubiquitous coverage are fundamentals requirements. Long term asset tracking definitely falls within such niches, and in particular NB-IoT can become a valuable alternative to be exploited by both replacing the conventional Global Position System (GPS) system, or supporting it. To this end, this paper proposes an innovative tracking system prototype for asset shipping which relies on two enabling technologies: GPS and NB-IoT. While position transmission is always put into effect via NB-IoT, it can be fetched by resorting to both GPS (like a standard tracker) or NB-IoT (thus establishing a GPS-less method). As a result, two localization techniques are arranged: the former one is preciser but energy hungrier, while the latter one is coarser but more low power. Such working principles were successfully tested on the field by means of two road tests in as much itineraries. Tests results are in agreement with the expectations underlying the two working principles since the finer one provides a more accurate tracking. In addition, a consumption analysis was also performed aiming at assessing the prototype lifetime. Finally, tests pursuing the assessment of the tracking error were carried out underling the fact that it strongly depends on the geographic deployment of NB-IoT towers

Offshore LoRaWAN Networking: Transmission Performances Analysis Under Different Environmental Conditions

This paper presents the architecture and the performances of a Long Range Wide Area Network (LoRaWAN) infrastructure used for data transmission from a floating sensor node placed in the middle of the sea to a multi-Gateway structure positioned on the coast, whose purpose is the monitoring of offshore breeding cages within a fish farming plant. In particular, the sensor node is installed on a seamark buoy and it is interfaced with ad hoc sensors for the measurement of marine parameters. All sampled data are conveyed ashore to the Gateways by successfully covering the distance of 8.33 km. The paper presents the results concerning the performances of the data transmission for a 70 days operating period, analyzing the radio parameters (Signal-to-Noise Ratio - SNR and Received Signal Strength Indicator - RSSI) in relation with the variations of environmental parameters like temperature, relative humidity and atmospheric pressure as well as weather conditions. The proposed system demonstrates the usability of the LoRaWAN in all those cases where data collection from offshore monitoring structures is required: while this infrastructure focuses on the monitoring of fish farming plants, it may find application in several contexts, from navigational buoys to offshore oil plants

Providing Energy Self-Sufficiency to LoRaWAN Nodes by Means of Thermoelectric Generators (TEGs)-Based Energy Harvesting

The aim of this paper is to present the viability of an energy-harvesting system prototype, based on thermoelectric generators (TEGs), to be embedded in a Long-Range Wide Area Network (LoRaWAN)-based wireless sensor node, allowing continuous quasi-real-time data transmission even for low temperature gradients and for frequent transmissions. To this end, an RFM95x LoRa module is used in the system. The energy management of the entire node is achieved by exploiting a nano power boost charger buck converter integrated circuit, which allows power extraction from the energy-harvesting source and, at the same time, regulates the charging/discharging process of a Li-Po battery that supplies the wireless node. The first phase of the project was dedicated to understanding the electrical characteristics of the TEG. A series of tests were performed to study the open circuit voltage, the current and the power generated by the TEG at different temperature gradients. Following this first phase, tests were then set up to study the charging/discharging process of the battery by changing two crucial parameters: the temperature between the faces of the TEG and the frequency of the transmissions performed by the transceiver. Experimental results show a positive balance for the battery charging at different conditions, which suggests two important conclusions: first of all, with high temperature gradients, it is possible to set relatively high transmission frequencies for the LoRaWAN module without discharging the battery. The second important consideration concerns the operation of the system at extremely low temperature gradients, with a minimum of 5 °C reached during one of the measurements. This suggests the usability of thermoelectric energy-harvesting systems in a wide range of possible applications even in conditions of low temperature gradients