Isolation of Mesenchymal Stem Cells from Murine Bone Marrow

Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the potential to differentiate into various cells lineages, including adipocytes, chondrocytes, osteocytes, and fibroblasts. Due to their ability to regenerate damaged tissues, MSCs have the potential to be the future of new cell-based therapies. However, identification of MSCs has not been standardized. The purpose of this project was to identify parameters for isolating MSCs from murine bone marrow. This was accomplished by combining a staining protocol that utilizes the unique surface characteristics of MSCs with the negative selection for CD45 and Ter-119, and then the negative selection for CD44 in the remaining cell population

Recruitment and Energy Audits of SMEs in Reigate

This study investigated different methods for engaging small and medium enterprises (SMEs) in the Borough of Reigate and Banstead to participate in energy audits. Energy audits were conducted of interested businesses and the team developed individualized reports for each audited SME. The team analyzed the audit and solicitation results in order to provide insights for the Council to continue effectively reducing the energy usage of small businesses and increase future participation