38,611 research outputs found
Neuronal Expression of Neural Nitric Oxide Synthase (nNOS) Protein is Suppressed by an Antisense RNA Transcribed from an NOS Pseudogene
Here, we show that a nitric oxide synthase (NOS) pseudogene is expressed in the CNS of the snail Lymnaea stagnalis. The pseudo-NOS transcript includes a region of significant antisense homology to a previously reported neuronal NOS (nNOS)-encoding mRNA. This suggested that the pseudo-NOS transcript acts as a natural antisense regulator of nNOS protein synthesis. In support of this, we show that both the nNOS-encoding and the pseudo-NOS transcripts are coexpressed in giant identified neurons (the cerebral giant cells) in the cerebral ganglion. Moreover, reverse transcription-PCR experiments on RNA isolated from the CNS establish that stable RNA-RNA duplex molecules do form between the two transcripts in vivo. Using an in vitro translation assay, we further demonstrate that the antisense region of the pseudogene transcript prevents the translation of nNOS protein from the nNOS-encoding mRNA. By analyzing NOS RNA and nNOS protein expression in two different identified neurons, we find that when both the nNOS-encoding and the pseudo-NOS transcripts are present in the same neuron, nNOS enzyme activity is substantially suppressed. Importantly, these results show that a natural antisense mechanism can mediate the translational control of nNOS expression in the Lymnaea CNS. Our findings also suggest that transcribed pseudogenes are not entirely without purpose and are a potential source of a new class of regulatory gene in the nervous system
Anterograde Signalling by Nitric Oxide: Characterisation and In Vitro Reconstitution of an Identified Nitrergic Synapse
Nitric oxide (NO) is recognized as a signaling molecule in the CNS where it is a candidate retrograde neurotransmitter. Here we provide direct evidence that NO mediates slow excitatory anterograde transmission between the NO synthase (NOS)-expressing B2 neuron and an NO-responsive follower neuron named B7nor. Both are motoneurons located in the buccal ganglia of the snail Lymnaea stagnalis where they participate in feeding behavior. Transmission between B2 and B7nor is blocked by inhibiting NOS and is suppressed by extracellular scavenging of NO. Furthermore, focal application of NO to the cell body of the B7nor neuron causes a depolarization that mimics the effect of B2 activity. The slow interaction between the B2 and B7nor neurons can be re-established when the two neurons are cocultured, and it shows the same susceptibility to NOS inhibition and NO scavenging. In cell culture we have also examined spatial aspects of NO signaling. We show that before the formation of an anatomical connection, the presynaptic neuron can cause depolarizing potentials in the follower neuron at distances up to 50 micro(m). The strength of the interaction increases when the distance between the cells is reduced. Our results suggest that NO can function as both a synaptic and a nonsynaptic signaling molecul
Gaussian benchmark for optical communication aiming towards ultimate capacity
We establish the fundamental limit of communication capacity within Gaussian
schemes under phase-insensitive Gaussian channels, which employ multimode
Gaussian states for encoding and collective Gaussian operations and
measurements for decoding. We prove that this Gaussian capacity is additive,
i.e., its upper bound occurs with separable encoding and separable receivers so
that a single-mode communication suffices to achieve the largest capacity under
Gaussian schemes. This rigorously characterizes the gap between the ultimate
Holevo capacity and the capacity within Gaussian communication, showing that
Gaussian regime is not sufficient to achieve the Holevo bound particularly in
the low-photon regime. Furthermore the Gaussian benchmark established here can
be used to critically assess the performance of non-Gaussian protocols for
optical communication. We move on to identify non-Gaussian schemes to beat the
Gaussian capacity and show that a non-Gaussian receiver recently implemented by
Becerra et al. [Nat. Photon. 7, 147 (2013)] can achieve this aim with an
appropriately chosen encoding strategy.Comment: 9 pages, 6 figures, with supplemental materia
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